PMID- 26950121
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20190110
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 17
IP  - 3
DP  - 2016 Mar 3
TI  - Functionalized Multi-Wall Carbon Nanotubes Enhance Transfection and Expression 
      Efficiency of Plasmid DNA in Fish Cells.
PG  - 335
LID - 10.3390/ijms17030335 [doi]
LID - 335
AB  - DNA vaccines are considered to be the most promising method against infectious 
      diseases in the aquaculture industry. In the present study, we investigated the 
      potency of ammonium group-functionalized multi-walled carbon nanotubes (MWCNTs) 
      in enhancing the transfection and expression efficiency of plasmid DNA 
      (pEGFP-vp5) in Ctenopharyngodon idellus kidney (CIK) cells. Agarose gel shift 
      assay results show that ammonium group-functionalized carbon nanotubes are able 
      to condense DNA in varying degrees. Scanning electron microscope (SEM) images 
      shows that CIK cells show a great affinity for MWCNTs-NH(3)(+) and the CNTs covering 
      the cell surface tend to orient their tips perpendicularly to the cell surface, 
      and appear to be "needle-pricking the cells". Transmission electron microscope 
      (TEM) images confirmed that MWCNTs-NH(3)(+) penetrate the cell membranes and are 
      widely dispersed in the CIK cell. Real-time PCR was used to detect the 
      transfection efficiency through the expression of the outer capsid protein (VP5). 
      The results showed that the MWCNTs-NH(3)(+):DNA complexes are able to transfect CIK 
      cells effectively at different charge ratio than naked DNA. Subsequent studies 
      confirmed that both functional groups and charge ratio are important factors that 
      determine the transfection efficiency of plasmid DNA. All these results indicated 
      that MWCNTs-NH(3)(+):DNA complexes could be suitable for developing DNA vaccine for 
      the control of virus infection in the aquaculture industry.
FAU - Liu, Guanglu
AU  - Liu G
AD  - College of Science, Northwest A & F University, Xinong Road 22nd, Yangling 
      712100, China. 563310592@163.com.
FAU - Wang, Yuan
AU  - Wang Y
AD  - College of Animal Science and Technology, Northwest A & F University, Xinong 
      Road 22nd, Yangling 712100, China. 18792426961@163.com.
FAU - Hu, Yang
AU  - Hu Y
AD  - College of Science, Northwest A & F University, Xinong Road 22nd, Yangling 
      712100, China. 18729316235@163.com.
FAU - Yu, Xiaobo
AU  - Yu X
AD  - College of Animal Science and Technology, Northwest A & F University, Xinong 
      Road 22nd, Yangling 712100, China. yuxiaobo6@126.com.
FAU - Zhu, Bin
AU  - Zhu B
AD  - College of Animal Science and Technology, Northwest A & F University, Xinong 
      Road 22nd, Yangling 712100, China. zhubin1227@126.com.
FAU - Wang, Gaoxue
AU  - Wang G
AD  - College of Animal Science and Technology, Northwest A & F University, Xinong 
      Road 22nd, Yangling 712100, China. wanggaoxue@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160303
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Ammonium Compounds)
RN  - 0 (Capsid Proteins)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Vaccines, DNA)
SB  - IM
MH  - Ammonium Compounds/*chemistry
MH  - Animals
MH  - Aquaculture
MH  - Capsid Proteins/genetics
MH  - Carps/*genetics/virology
MH  - Cells, Cultured
MH  - Fish Diseases/prevention & control/virology
MH  - *Gene Expression
MH  - Kidney/ultrastructure
MH  - Microscopy, Electron, Scanning
MH  - Nanotubes, Carbon/*chemistry
MH  - Organisms, Genetically Modified
MH  - Plasmids/*genetics/ultrastructure
MH  - *Transfection
MH  - Vaccines, DNA/genetics
PMC - PMC4813197
OTO - NOTNLM
OT  - DNA vaccine
OT  - grass carp reovirus (GCRV)
OT  - multi-wall carbon nanotubes (MWCNTs)
OT  - outer capsid protein VP5
EDAT- 2016/03/08 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/03/01
CRDT- 2016/03/08 06:00
PHST- 2015/12/31 00:00 [received]
PHST- 2016/01/29 00:00 [revised]
PHST- 2016/02/24 00:00 [accepted]
PHST- 2016/03/08 06:00 [entrez]
PHST- 2016/03/08 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - ijms17030335 [pii]
AID - ijms-17-00335 [pii]
AID - 10.3390/ijms17030335 [doi]
PST - epublish
SO  - Int J Mol Sci. 2016 Mar 3;17(3):335. doi: 10.3390/ijms17030335.