PMID- 26954083
OWN - NLM
STAT- MEDLINE
DCOM- 20161227
LR  - 20180417
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 142
DP  - 2016 Jun 1
TI  - Orientating lipase molecules through surface chemical control for enhanced 
      activity: A QCM-D and ToF-SIMS investigation.
PG  - 173-181
LID - S0927-7765(16)30138-2 [pii]
LID - 10.1016/j.colsurfb.2016.02.059 [doi]
AB  - Bio-active materials consisting of lipase encapsulated within porous silica 
      particles were engineered to control the adsorption kinetics and molecular 
      orientation of lipase, which play critical roles in the digestion kinetics of 
      triglycerides. The adsorption kinetics of Candida antartica lipase A (CalA) was 
      monitored using quartz crystal microbalance with dissipation (QCM-D) and 
      controlled by altering the hydrophobicity of a silica binding support. The extent 
      of adsorption was 2-fold greater when CalA was adsorbed onto hydrophobic silica 
      compared to hydrophilic silica. Time-of-flight secondary ion mass spectrometry 
      (ToF-SIMS) fragmentation patterns, in conjunction with multivariate statistics, 
      demonstrated enhanced exposure of the lipase's catalytic domain, specifically the 
      histidine group responsible for activity, when CalA was adsorbed on hydrophilic 
      silica. Consequently, lipid digestion kinetics were enhanced when CalA was loaded 
      in hydrophilic porous silica particles, i.e., a 2-fold increase in the 
      pseudo-first-order rate constant for digestion when compared to free lipase. In 
      contrast, digestion kinetics were inhibited when CalA was hosted in hydrophobic 
      porous silica, i.e., a 5-fold decrease in pseudo-first-order rate constant for 
      digestion when compared to free lipase. These findings provide valuable insights 
      into the mechanism of lipase action which can be exploited to develop smarter 
      food and drug delivery systems consisting of porous lipid-based materials.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Joyce, Paul
AU  - Joyce P
AD  - School of Pharmacy and Medical Sciences, University of South Australia, City East 
      Campus, Adelaide, South Australia 5001, Australia.
FAU - Kempson, Ivan
AU  - Kempson I
AD  - Future Industries Institute, University of South Australia, Mawson Lakes Campus, 
      Mawson Lakes, South Australia 5095, Australia.
FAU - Prestidge, Clive A
AU  - Prestidge CA
AD  - School of Pharmacy and Medical Sciences, University of South Australia, City East 
      Campus, Adelaide, South Australia 5001, Australia. Electronic address: 
      clive.prestidge@unisa.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160227
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Fungal Proteins)
RN  - 0 (Triglycerides)
RN  - 4QD397987E (Histidine)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Adsorption
MH  - Candida/chemistry/enzymology
MH  - Catalytic Domain
MH  - Drug Compounding
MH  - Drug Delivery Systems
MH  - Fungal Proteins/*chemistry/isolation & purification
MH  - Histidine/chemistry
MH  - Hydrolysis
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Kinetics
MH  - Lipase/*chemistry/isolation & purification
MH  - Porosity
MH  - Quartz Crystal Microbalance Techniques
MH  - Silicon Dioxide/*chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Surface Properties
MH  - Triglycerides/*chemistry
OTO - NOTNLM
OT  - Lipase
OT  - Lipid digestion
OT  - Porous silica
OT  - QCM-D
OT  - Surface chemistry
OT  - ToF-SIMS
EDAT- 2016/03/10 06:00
MHDA- 2016/12/28 06:00
CRDT- 2016/03/09 06:00
PHST- 2015/11/06 00:00 [received]
PHST- 2016/02/23 00:00 [revised]
PHST- 2016/02/25 00:00 [accepted]
PHST- 2016/03/09 06:00 [entrez]
PHST- 2016/03/10 06:00 [pubmed]
PHST- 2016/12/28 06:00 [medline]
AID - S0927-7765(16)30138-2 [pii]
AID - 10.1016/j.colsurfb.2016.02.059 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2016 Jun 1;142:173-181. doi: 
      10.1016/j.colsurfb.2016.02.059. Epub 2016 Feb 27.