PMID- 26954088
OWN - NLM
STAT- MEDLINE
DCOM- 20161227
LR  - 20180417
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 142
DP  - 2016 Jun 1
TI  - Delivery of fluorophores by calcium phosphate-coated nanoliposomes and 
      interaction with Staphylococcus aureus biofilms.
PG  - 214-222
LID - S0927-7765(16)30147-3 [pii]
LID - 10.1016/j.colsurfb.2016.03.003 [doi]
AB  - The delivery capacity and mechanical stability of calcium phosphate (CaP) coated 
      1,2-dioleoyl-sn-glycero-3-phosphate (DOPA) liposomes free and adsorbed on 
      bacterial surface was investigated introducing either acridine orange (AO) or 
      5,10,15,20-Tetrakis(1-methyl-4-pyridinio)porphyrin (TMP) in the aqueous core of 
      the liposomes. The obtained nanomaterials were thoroughly characterized by 
      electron and optical microscopy and by fluorescence techniques. Distribution of 
      the AO and TMP molecules between the aqueous liposomes core and the outer 
      solution was demonstrated by the band shifts and broadening of the 
      excitation-emission matrices and the modified Stern-Volmer model for fluorescence 
      quenching. In aqueous suspensions, c.a. 40% of AO was released to the outer 
      solution while only a small percentage of TMP was observed to reach the outer 
      liposome surface. The nanoliposomes adhesion capacity and the leaking of 
      fluorophore molecules to Staphylococcus aureus (S. aureus) biofilms were further 
      evaluated. A close interaction between liposomes and S. aureus biofilm was 
      evidenced by TEM and SEM imaging. Epifluorescence experiments demonstrated that 
      CaP-coated liposomes have good biofilm staining capability after two hours 
      incubation of the biofilms with the liposomes, thus supporting an important 
      release of the fluorophores when in contact with the biofilm. Altogether, the 
      obtained results strongly suggest that CaP-coated liposomes are capable of 
      activating drug release when in presence of S. aureus biofilms and smears. The 
      studies herein presented, indicate that CaP-coated liposomes are potential 
      vehicles for the selective delivery of drugs to S. aureus biofilms, as is the 
      case of the singlet oxygen photosensitizer TMP, a well known photodynamic 
      antibacterial agent.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Rivero Berti, Ignacio
AU  - Rivero Berti I
AD  - Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas, Facultad de 
      Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 16, 
      Sucursal 4, 1900 La Plata Argentina.
FAU - Dell' Arciprete, Maria Laura
AU  - Dell' Arciprete ML
AD  - Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas, Facultad de 
      Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 16, 
      Sucursal 4, 1900 La Plata Argentina. Electronic address: 
      mlaura@inifta.unlp.edu.ar.
FAU - Dittler, Maria Laura
AU  - Dittler ML
AD  - Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas, Facultad de 
      Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 16, 
      Sucursal 4, 1900 La Plata Argentina.
FAU - Minan, Alejandro
AU  - Minan A
AD  - Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas, Facultad de 
      Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 16, 
      Sucursal 4, 1900 La Plata Argentina.
FAU - Fernandez Lorenzo de Mele, Monica
AU  - Fernandez Lorenzo de Mele M
AD  - Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas, Facultad de 
      Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 16, 
      Sucursal 4, 1900 La Plata Argentina.
FAU - Gonzalez, Monica
AU  - Gonzalez M
AD  - Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas, Facultad de 
      Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 16, 
      Sucursal 4, 1900 La Plata Argentina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160302
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Calcium Phosphates)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Liposomes)
RN  - 0 (Phosphatidic Acids)
RN  - 0 (Porphyrins)
RN  - 0 (Suspensions)
RN  - 0 (meso-tetrakis(1-methyl-4-pyridiniumyl)porphyrin)
RN  - 059QF0KO0R (Water)
RN  - 14268-17-8 (dioleoylphosphatidic acid)
RN  - 97Z1WI3NDX (calcium phosphate)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/chemistry
MH  - Adsorption
MH  - Biofilms/*drug effects/growth & development
MH  - Calcium Phosphates/*chemistry
MH  - Drug Compounding
MH  - Drug Liberation
MH  - Fluorescent Dyes/chemistry
MH  - Kinetics
MH  - Liposomes/*chemistry/pharmacology
MH  - Microscopy, Electron, Transmission
MH  - Nanostructures/*chemistry
MH  - Phosphatidic Acids/chemistry
MH  - Porphyrins/chemistry
MH  - Spectrometry, Fluorescence
MH  - Staphylococcus aureus/*drug effects/physiology
MH  - Suspensions
MH  - Water/chemistry
OTO - NOTNLM
OT  - 5, 10, 15, 20-Tetrakis(1-methyl-4-pyridinio)porphyrin
OT  - Acridine orange
OT  - Calcium phosphate
OT  - Nanoliposomes
OT  - Staphylococcus aureus
EDAT- 2016/03/10 06:00
MHDA- 2016/12/28 06:00
CRDT- 2016/03/09 06:00
PHST- 2015/11/25 00:00 [received]
PHST- 2016/02/22 00:00 [revised]
PHST- 2016/03/01 00:00 [accepted]
PHST- 2016/03/09 06:00 [entrez]
PHST- 2016/03/10 06:00 [pubmed]
PHST- 2016/12/28 06:00 [medline]
AID - S0927-7765(16)30147-3 [pii]
AID - 10.1016/j.colsurfb.2016.03.003 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2016 Jun 1;142:214-222. doi: 
      10.1016/j.colsurfb.2016.03.003. Epub 2016 Mar 2.