PMID- 26954158
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160629
LR  - 20200929
IS  - 1011-2367 (Print)
IS  - 1976-5517 (Electronic)
IS  - 1011-2367 (Linking)
VI  - 29
IP  - 8
DP  - 2016 Aug
TI  - Rapamycin Influences the Efficiency of In vitro Fertilization and Development in 
      the Mouse: A Role for Autophagic Activation.
PG  - 1102-10
LID - 10.5713/ajas.15.0762 [doi]
AB  - The mammalian target of rapamycin (mTOR) regulates cellular processes such as 
      cell growth, metabolism, transcription, translation, and autophagy. Rapamycin is 
      a selective inhibitor of mTOR, and induces autophagy in various systems. 
      Autophagy contributes to clearance and recycling of macromolecules and organelles 
      in response to stress. We previously reported that vitrified-warmed mouse oocytes 
      show acute increases in autophagy during warming, and suggested that it is a 
      natural response to cold stress. In this follow-up study, we examined whether the 
      modulation of autophagy influences survival, fertilization, and developmental 
      rates of vitrified-warmed mouse oocytes. We used rapamycin to enhance autophagy 
      in metaphase II (MII) oocytes before and after vitrification. The oocytes were 
      then subjected to in vitro fertilization (IVF). The fertilization and 
      developmental rates of vitrified-warmed oocytes after rapamycin treatment were 
      significantly lower than those for control groups. Modulation of autophagy with 
      rapamycin treatment shows that rapamycin-induced autophagy exerts a negative 
      influence on fertilization and development of vitrified-warmed oocytes.
FAU - Lee, Geun-Kyung
AU  - Lee GK
AD  - Department of Biomedical Science and Technology, Institute of Biomedical Science 
      and Technology, Konkuk University, Seoul 143-701, Korea.
FAU - Shin, Hyejin
AU  - Shin H
AD  - Department of Biomedical Science and Technology, Institute of Biomedical Science 
      and Technology, Konkuk University, Seoul 143-701, Korea.
FAU - Lim, Hyunjung Jade
AU  - Lim HJ
AD  - Department of Biomedical Science and Technology, Institute of Biomedical Science 
      and Technology, Konkuk University, Seoul 143-701, Korea.
AD  - Department of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.
LA  - eng
PT  - Journal Article
DEP - 20151008
PL  - Korea (South)
TA  - Asian-Australas J Anim Sci
JT  - Asian-Australasian journal of animal sciences
JID - 9884245
PMC - PMC4932563
OTO - NOTNLM
OT  - Development
OT  - Mouse
OT  - Oocyte
OT  - Rapamycin
OT  - Vitrification
EDAT- 2016/03/10 06:00
MHDA- 2016/03/10 06:01
PMCR- 2016/08/01
CRDT- 2016/03/09 06:00
PHST- 2015/09/11 00:00 [received]
PHST- 2015/10/01 00:00 [revised]
PHST- 2015/10/07 00:00 [accepted]
PHST- 2016/03/09 06:00 [entrez]
PHST- 2016/03/10 06:00 [pubmed]
PHST- 2016/03/10 06:01 [medline]
PHST- 2016/08/01 00:00 [pmc-release]
AID - ajas.15.0762 [pii]
AID - ajas-29-8-1102 [pii]
AID - 10.5713/ajas.15.0762 [doi]
PST - ppublish
SO  - Asian-Australas J Anim Sci. 2016 Aug;29(8):1102-10. doi: 10.5713/ajas.15.0762. 
      Epub 2015 Oct 8.