PMID- 26955366
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160309
LR  - 20200929
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 7
DP  - 2016
TI  - SiaA/D Interconnects c-di-GMP and RsmA Signaling to Coordinate Cellular 
      Aggregation of Pseudomonas aeruginosa in Response to Environmental Conditions.
PG  - 179
LID - 10.3389/fmicb.2016.00179 [doi]
LID - 179
AB  - Pseudomonas aeruginosa has emerged as an important opportunistic human pathogen 
      that is often highly resistant to eradication strategies, mediated in part by the 
      formation of multicellular aggregates. Cellular aggregates may occur attached to 
      a surface (biofilm), at the air-liquid interface (pellicle), or as suspended 
      aggregates. Compared to surface attached communities, knowledge about the 
      regulatory processes involved in the formation of suspended cell aggregates is 
      still limited. We have recently described the SiaA/D signal transduction module 
      that regulates macroscopic cell aggregation during growth with, or in the 
      presence of the surfactant SDS. Targets for SiaA/D mediated regulation include 
      the Psl polysaccharide, the CdrAB two-partner secretion system and the CupA 
      fimbriae. While the global regulators c-di-GMP and RsmA are known to inversely 
      coordinate cell aggregation and regulate the expression of several adhesins, 
      their potential impact on the expression of the cupA operon remains unknown. 
      Here, we investigated the function of SiaA (a putative ser/thr phosphatase) and 
      SiaD (a di-guanylate cyclase) in cupA1 expression using transcriptional reporter 
      fusions and qRT-PCR. These studies revealed a novel interaction between the RsmA 
      posttranscriptional regulatory system and SiaA/D mediated macroscopic 
      aggregation. The RsmA/rsmY/Z system was found to affect macroscopic aggregate 
      formation in the presence of surfactant by impacting the stability of the cupA1 
      mRNA transcript and we reveal that RsmA directly binds to the cupA1 leader 
      sequence in vitro. We further identified that transcription of the RsmA 
      antagonist rsmZ is controlled in a SiaA/D dependent manner during growth with 
      SDS. Finally, we found that the siaD transcript is also under regulatory control 
      of RsmA and that overproduction of RsmA or the deletion of siaD results in 
      decreased cellular cyclic di-guanosine monophosphate (c-di-GMP) levels quantified 
      by a transcriptional reporter, demonstrating that SiaA/D connects c-di-GMP and 
      RsmA/rsmY/Z signaling to reciprocally regulate cell aggregation in response to 
      environmental conditions.
FAU - Colley, Brendan
AU  - Colley B
AD  - Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular 
      Sciences, University of New South Wales Sydney, NSW, Australia.
FAU - Dederer, Verena
AU  - Dederer V
AD  - Institute of Technical Biochemistry, University of Stuttgart Stuttgart, Germany.
FAU - Carnell, Michael
AU  - Carnell M
AD  - Biomedical Image Facility, Mark Wainwright Analytical Centre, University of New 
      South Wales Sydney, NSW, Australia.
FAU - Kjelleberg, Staffan
AU  - Kjelleberg S
AD  - Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular 
      Sciences, University of New South WalesSydney, NSW, Australia; Singapore Centre 
      for Environmental Life Sciences Engineering and School of Biological Sciences, 
      Nanyang Technological University, SingaporeSingapore.
FAU - Rice, Scott A
AU  - Rice SA
AD  - Centre for Marine Bio-Innovation, School of Biotechnology and Biomolecular 
      Sciences, University of New South WalesSydney, NSW, Australia; Singapore Centre 
      for Environmental Life Sciences Engineering and School of Biological Sciences, 
      Nanyang Technological University, SingaporeSingapore.
FAU - Klebensberger, Janosch
AU  - Klebensberger J
AD  - Institute of Technical Biochemistry, University of Stuttgart Stuttgart, Germany.
LA  - eng
PT  - Journal Article
DEP - 20160226
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC4768041
OTO - NOTNLM
OT  - CupA fimbriae
OT  - Pseudomonas aeruginosa
OT  - RsmA
OT  - SiaA
OT  - SiaD
OT  - biofilm
OT  - c-di-GMP signaling
OT  - cellular aggregation
EDAT- 2016/03/10 06:00
MHDA- 2016/03/10 06:01
PMCR- 2016/02/26
CRDT- 2016/03/09 06:00
PHST- 2015/11/26 00:00 [received]
PHST- 2016/02/02 00:00 [accepted]
PHST- 2016/03/09 06:00 [entrez]
PHST- 2016/03/10 06:00 [pubmed]
PHST- 2016/03/10 06:01 [medline]
PHST- 2016/02/26 00:00 [pmc-release]
AID - 10.3389/fmicb.2016.00179 [doi]
PST - epublish
SO  - Front Microbiol. 2016 Feb 26;7:179. doi: 10.3389/fmicb.2016.00179. eCollection 
      2016.