PMID- 26956786
OWN - NLM
STAT- MEDLINE
DCOM- 20170109
LR  - 20170110
IS  - 1708-8267 (Electronic)
IS  - 1081-5589 (Linking)
VI  - 64
IP  - 4
DP  - 2016 Apr
TI  - Detection of chromosomal changes in chronic lymphocytic leukemia using classical 
      cytogenetic methods and FISH: application of rich mitogen mixtures for lymphocyte 
      cultures.
PG  - 894-8
LID - 10.1136/jim-2015-000050 [doi]
AB  - One of the research methods of prognostic value in chronic lymphocytic leukemia 
      (CLL) is cytogenetic analysis. This method requires the presence of appropriate 
      B-cell mitogens in cultures in order to obtain a high mitotic index. The aim of 
      our research was to determine the most effective methods of in vitro B-cell 
      stimulation to maximize the number of metaphases from peripheral blood cells of 
      patients with CLL for classical cytogenetic examination, and then to correlate 
      the results with those obtained using fluorescence in situ hybridization (FISH). 
      The study group involved 50 consecutive patients with CLL. Cell cultures were 
      maintained with the basic composition of culture medium and addition of 
      respective stimulators. We used the following stimulators: Pokeweed Mitogen 
      (PWM), 12-O-tetradecanoylphorbol 13-acetate (TPA), ionophore, lipopolysaccharide 
      (LPS), and CpG-oligonucleotide DSP30. We received the highest mitotic index when 
      using the mixture of PWM+TPA+I+DSP30. With classical cytogenetic tests using 
      banding techniques, numerical and structural aberrations of chromosomes were 
      detected in 46 patients, and no change was found in only four patients. Test 
      results clearly confirmed the legitimacy of using cell cultures enriched with the 
      mixture of cell stimulators and combining classical cytogenetic techniques with 
      the FISH technique in later patient diagnosing.
CI  - Copyright (c) 2016 American Federation for Medical Research.
FAU - Koczkodaj, Dorota
AU  - Koczkodaj D
AD  - Department of Cancer Genetics, Medical University of Lublin, Lublin, Poland.
FAU - Popek, Sylwia
AU  - Popek S
AD  - Department of Cancer Genetics, Medical University of Lublin, Lublin, Poland.
FAU - Zmorzynski, Szymon
AU  - Zmorzynski S
AD  - Department of Cancer Genetics, Medical University of Lublin, Lublin, Poland.
FAU - Wasik-Szczepanek, Ewa
AU  - Wasik-Szczepanek E
AD  - Department of Hematooncology and Bone Marrow Transplantation, Medical University 
      of Lublin, Lublin, Poland.
FAU - Filip, Agata A
AU  - Filip AA
AD  - Department of Cancer Genetics, Medical University of Lublin, Lublin, Poland.
LA  - eng
PT  - Journal Article
DEP - 20160308
PL  - England
TA  - J Investig Med
JT  - Journal of investigative medicine : the official publication of the American 
      Federation for Clinical Research
JID - 9501229
RN  - 0 (Mitogens)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Cytogenetic Analysis/*methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Lymphocytes/drug effects/*metabolism
MH  - Male
MH  - Metaphase/drug effects
MH  - Middle Aged
MH  - Mitogens/*pharmacology
MH  - Mitotic Index
OTO - NOTNLM
OT  - Cell Proliferation
OT  - Hematologic Diseases
OT  - Leukemia
EDAT- 2016/03/10 06:00
MHDA- 2017/01/10 06:00
CRDT- 2016/03/10 06:00
PHST- 2016/02/13 00:00 [accepted]
PHST- 2016/03/10 06:00 [entrez]
PHST- 2016/03/10 06:00 [pubmed]
PHST- 2017/01/10 06:00 [medline]
AID - jim-2015-000050 [pii]
AID - 10.1136/jim-2015-000050 [doi]
PST - ppublish
SO  - J Investig Med. 2016 Apr;64(4):894-8. doi: 10.1136/jim-2015-000050. Epub 2016 Mar 
      8.