PMID- 26962520
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160310
LR  - 20200929
IS  - 2277-9175 (Print)
IS  - 2277-9175 (Electronic)
IS  - 2277-9175 (Linking)
VI  - 5
DP  - 2016
TI  - Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter 
      region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan 
      population.
PG  - 18
LID - 10.4103/2277-9175.175904 [doi]
LID - 18
AB  - BACKGROUND: Type 2 diabetes mellitus (T2DM) is an inflammatory autoimmune disease 
      that mostly affects older adults. The etiology of T2DM includes both genetic and 
      environmental factors. rs1800795 (-174 G/C) single nucleotide polymorphism (SNP) 
      linked with autoimmune disorders predispositions, identified by Genome-Wide 
      Association Study among genes, which immunologically related is considerably over 
      signified. The goal of this study was to evaluate the association between 
      rs1800795 (-174 G/C) polymorphisms in the promoter of interleukin-6 (IL-6) gene 
      with susceptibility to T2DM in a subset of the Iranian population. MATERIALS AND 
      METHODS: In this case-control study, 120 healthy subjects and 120 patients with 
      T2DM were included. Genomic DNA obtained from whole blood samples and the 
      polymerase chain reaction was used to amplify the fragment of interest contain 
      rs1800795 SNP, restriction fragment length polymorphism method was applied for 
      genotyping of the DNA samples with NlaIII as a restriction enzyme. SPSS for 
      Windows software (version 18.0, SPSS, Chicago, IL, USA) was performed for 
      statistical analysis. RESULTS: No significant differences were found between 
      healthy controls and T2DM patients with respect to the frequency distribution of 
      the cytokine gene polymorphism investigated. Odds ratio, adjusted for sex, age, 
      and smoking status has displayed similar outcomes. CONCLUSION: These results 
      indicated that the rs1800795 SNP is not a susceptibility gene variant for the 
      development of T2DM in the Isfahan population. Further studies using new data on 
      complex transcriptional interactions between IL-6 polymorphic sites are necessary 
      to determine IL-6 haplotype influence on susceptibility to T2DM.
FAU - Ghavimi, Reza
AU  - Ghavimi R
AD  - Department of Pharmaceutical Biotechnology, Isfahan University of Medical 
      Sciences, Isfahan, Iran.
FAU - Sharifi, Mohammadreza
AU  - Sharifi M
AD  - Department of Genetics and Molecular Biology, Isfahan University of Medical 
      Sciences, Isfahan, Iran.
FAU - Mohaghegh, Mohammad Ali
AU  - Mohaghegh MA
AD  - Department of Parasitology and Mycology, Isfahan University of Medical Sciences, 
      Isfahan, Iran.
FAU - Mohammadian, Hossein
AU  - Mohammadian H
AD  - Department of Pharmaceutical Biotechnology, Isfahan University of Medical 
      Sciences, Isfahan, Iran.
FAU - Khadempar, Saedeh
AU  - Khadempar S
AD  - Department of Biology, Kurdistan Science and Research Branch, Islamic Azad 
      University, Sanandaj, Iran.
FAU - Rezaei, Hamzeh
AU  - Rezaei H
AD  - Department of Clinical Biochemistry, School of Medicine, Zahedan University of 
      Medical Sciences, Zahedan, Iran.
LA  - eng
PT  - Journal Article
DEP - 20160208
PL  - India
TA  - Adv Biomed Res
JT  - Advanced biomedical research
JID - 101586897
PMC - PMC4770613
OTO - NOTNLM
OT  - Genome-wide association study
OT  - interleukin-6 gene
OT  - polymorphism
OT  - type 2 diabetes mellitus
EDAT- 2016/03/11 06:00
MHDA- 2016/03/11 06:01
PMCR- 2016/02/08
CRDT- 2016/03/11 06:00
PHST- 2015/06/03 00:00 [received]
PHST- 2015/07/07 00:00 [accepted]
PHST- 2016/03/11 06:00 [entrez]
PHST- 2016/03/11 06:00 [pubmed]
PHST- 2016/03/11 06:01 [medline]
PHST- 2016/02/08 00:00 [pmc-release]
AID - ABR-5-18 [pii]
AID - 10.4103/2277-9175.175904 [doi]
PST - epublish
SO  - Adv Biomed Res. 2016 Feb 8;5:18. doi: 10.4103/2277-9175.175904. eCollection 2016.