PMID- 26963101
OWN - NLM
STAT- MEDLINE
DCOM- 20160801
LR  - 20190219
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 3
DP  - 2016
TI  - Pathologic Evaluation of Type 2 Porcine Reproductive and Respiratory Syndrome 
      Virus Infection at the Maternal-Fetal Interface of Late Gestation Pregnant Gilts.
PG  - e0151198
LID - 10.1371/journal.pone.0151198 [doi]
LID - e0151198
AB  - The pathogenesis of fetal death caused by porcine reproductive and respiratory 
      syndrome virus (PRRSV) remains unclear. The objective of this study was to 
      improve our understanding of the pathogenesis by assessing potential 
      relationships between specific histopathological lesions and PRRSV RNA 
      concentration in the fetuses and the maternal-fetal interface. Pregnant gilts 
      were inoculated with PRRSV (n = 114) or sham inoculated (n = 19) at 85+/-1 days of 
      gestation. Dams and their litters were humanely euthanized and necropsied 21 days 
      later. PRRSV RNA concentration was measured by qRT-PCR in the maternal-fetal 
      interface and fetal thymus (n = 1391). Presence of fetal lesions was positively 
      related to PRRSV RNA concentration in the maternal-fetal interface and fetal 
      thymus (P<0.05 for both), but not to the distribution or severity of vasculitis, 
      or the severity of endometrial inflammation. The presence of fetal and umbilical 
      lesions was associated with greater odds of meconium staining (P<0.05 for both). 
      The distribution and severity of vasculitis in endometrium were not significantly 
      related to PRRSV RNA concentration in maternal-fetal interface or fetal thymus. 
      Endometrial inflammation severity was positively related to distribution and 
      severity of vasculitis in endometrium (P<0.001 for both). Conclusions from this 
      study suggest that type 2 PRRSV infection in pregnant gilts induces significant 
      histopathological lesions at maternal-fetal interface, but they are not 
      associated with presence of PRRSV in the maternal-fetal interface at 21 days post 
      infection. Conversely, fetal pathological lesions are associated with presence of 
      PRRSV in the maternal-fetal interface and fetal thymus, and meconium staining is 
      significantly associated with the presence of both fetal and umbilical lesions 
      observed 21 days post infection.
FAU - Novakovic, Predrag
AU  - Novakovic P
AD  - Department of Veterinary Pathology, Western College of Veterinary Medicine, 
      University of Saskatchewan, Saskatoon, SK, Canada.
FAU - Harding, John C S
AU  - Harding JC
AD  - Department of Large Animal Clinical Sciences, Western College of Veterinary 
      Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
FAU - Al-Dissi, Ahmad N
AU  - Al-Dissi AN
AD  - Department of Veterinary Pathology, Western College of Veterinary Medicine, 
      University of Saskatchewan, Saskatoon, SK, Canada.
FAU - Ladinig, Andrea
AU  - Ladinig A
AD  - Department of Large Animal Clinical Sciences, Western College of Veterinary 
      Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
AD  - University Clinic for Swine, Department for Farm Animals and Veterinary Public 
      Health, University of Veterinary Medicine Vienna, Vienna, Austria.
FAU - Detmer, Susan E
AU  - Detmer SE
AD  - Department of Veterinary Pathology, Western College of Veterinary Medicine, 
      University of Saskatchewan, Saskatoon, SK, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160310
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Endometriosis/metabolism/pathology/virology
MH  - Female
MH  - Fetus/embryology/pathology/virology
MH  - Placenta/*metabolism/pathology/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*metabolism/pathology
MH  - Porcine respiratory and reproductive syndrome virus/*metabolism
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/*metabolism/pathology/*virology
MH  - RNA, Viral/*metabolism
MH  - Swine/metabolism/virology
MH  - Thymus Gland/embryology/pathology/virology
MH  - Vasculitis/metabolism/pathology/virology
PMC - PMC4786155
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/03/11 06:00
MHDA- 2016/08/02 06:00
PMCR- 2016/03/10
CRDT- 2016/03/11 06:00
PHST- 2015/11/27 00:00 [received]
PHST- 2016/02/24 00:00 [accepted]
PHST- 2016/03/11 06:00 [entrez]
PHST- 2016/03/11 06:00 [pubmed]
PHST- 2016/08/02 06:00 [medline]
PHST- 2016/03/10 00:00 [pmc-release]
AID - PONE-D-15-51777 [pii]
AID - 10.1371/journal.pone.0151198 [doi]
PST - epublish
SO  - PLoS One. 2016 Mar 10;11(3):e0151198. doi: 10.1371/journal.pone.0151198. 
      eCollection 2016.