PMID- 26968213
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20231111
IS  - 1559-4106 (Electronic)
IS  - 1934-8630 (Print)
IS  - 1559-4106 (Linking)
VI  - 11
IP  - 2
DP  - 2016 Jun 11
TI  - Differential surface activation of the A1 domain of von Willebrand factor.
PG  - 029803
LID - 10.1116/1.4943618 [doi]
LID - 029803
AB  - The clotting protein von Willebrand factor (VWF) binds to platelet receptor 
      glycoprotein Ibalpha (GPIbalpha) when VWF is activated by chemicals, high shear stress, 
      or immobilization onto surfaces. Activation of VWF by surface immobilization is 
      an important problem in the failure of cardiovascular implants, but is poorly 
      understood. Here, the authors investigate whether some or all surfaces can 
      activate VWF at least in part by affecting the orientation or conformation of the 
      immobilized GPIbalpha-binding A1 domain of VWF. Platelets binding to A1 adsorbed onto 
      polystyrene surfaces translocated rapidly at moderate and high flow, but detached 
      at low flow, while platelets binding to A1 adsorbed onto glass or tissue-culture 
      treated polystyrene surfaces translocated slowly, and detached only at high flow. 
      Both x-ray photoelectron spectroscopy and conformation independent antibodies 
      reported comparable A1 amounts on all surfaces. Time-of-flight secondary ion mass 
      spectrometry (ToF-SIMS) and near-edge x-ray absorption fine structure spectra 
      suggested differences in orientation on the three surfaces, but none that could 
      explain the biological data. Instead, ToF-SIMS data and binding of 
      conformation-dependent antibodies were consistent with the stabilization of an 
      alternative more activated conformation of A1 by tissue culture polystyrene and 
      especially glass. These studies demonstrate that different material surfaces 
      differentially affect the conformation of adsorbed A1 domain and its biological 
      activity. This is important when interpreting or designing in vitro experiments 
      with surface-adsorbed A1 domain, and is also of likely relevance for 
      blood-contacting biomaterials.
FAU - Tronic, Elaine H
AU  - Tronic EH
AD  - Department of Bioengineering, University of Washington, Seattle, Washington 
      98195.
FAU - Yakovenko, Olga
AU  - Yakovenko O
AD  - Department of Bioengineering, University of Washington, Seattle, Washington 
      98195.
FAU - Weidner, Tobias
AU  - Weidner T
AD  - Department of Bioengineering, University of Washington, Seattle, Washington 
      98195.
FAU - Baio, Joe E
AU  - Baio JE
AD  - Department of Chemical Engineering, University of Washington, Seattle, Washington 
      98195.
FAU - Penkala, Rebecca
AU  - Penkala R
AD  - Department of Bioengineering, University of Washington, Seattle, Washington 
      98195.
FAU - Castner, David G
AU  - Castner DG
AD  - Departments of Bioengineering and Chemical Engineering, University of Washington, 
      Seattle, Washington 98195.
FAU - Thomas, Wendy E
AU  - Thomas WE
AD  - Department of Bioengineering, University of Washington, Seattle, Washington 
      98195.
LA  - eng
GR  - P41 EB002027/EB/NIBIB NIH HHS/United States
GR  - R01 HL106074/HL/NHLBI NIH HHS/United States
GR  - 1R01 HL106074/HL/NHLBI NIH HHS/United States
GR  - EB-002027/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160611
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
RN  - 0 (Platelet Glycoprotein GPIb-IX Complex)
RN  - 0 (Polystyrenes)
RN  - 0 (von Willebrand Factor)
SB  - IM
MH  - Blood Platelets/*physiology
MH  - *Cell Adhesion
MH  - Glass
MH  - Humans
MH  - Platelet Glycoprotein GPIb-IX Complex/*metabolism
MH  - Polystyrenes
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - *Surface Properties
MH  - von Willebrand Factor/*metabolism
PMC - PMC4788635
EDAT- 2016/03/13 06:00
MHDA- 2016/10/14 06:00
PMCR- 2016/03/11
CRDT- 2016/03/13 06:00
PHST- 2016/03/13 06:00 [entrez]
PHST- 2016/03/13 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
PHST- 2016/03/11 00:00 [pmc-release]
AID - 1.4943618 [pii]
AID - 329602BIP [pii]
AID - 10.1116/1.4943618 [doi]
PST - epublish
SO  - Biointerphases. 2016 Jun 11;11(2):029803. doi: 10.1116/1.4943618.