PMID- 26969921
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160713
LR  - 20160313
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 30
IP  - 7
DP  - 2016 Apr 15
TI  - Single analyzer precursor scans using an ion trap.
PG  - 800-4
LID - 10.1002/rcm.7500 [doi]
AB  - RATIONALE: Precursor ion and neutral loss scans are general survey methods of 
      tandem mass spectrometry (MS/MS) used for detecting structurally related 
      compounds. Until now they have been performed in multiple analyzer instruments, 
      e.g. triple quadrupoles and hybrid MS/MS instruments. Implementation of precursor 
      ion scans in single mass analyzers would be advantageous in reducing instrument 
      complexity. METHODS: Adoption of secular frequency scanning as a method of 
      mass-selective excitation is shown to enable precursor scans in a single ion trap 
      in a miniature mass spectrometer. A small supplementary alternating current (ac) 
      signal is swept in frequency so as to cause mass-selective excitation of trapped 
      ions. Simultaneously, a higher fixed amplitude ac signal is applied at the fixed 
      secular frequency of a product ion, ejecting the mass-selected product ion and 
      providing temporal data corresponding to a precursor ion spectrum. RESULTS: 
      Precursor scanning in a single ion trap is demonstrated using a mixture of three 
      illicit drugs: cocaine, 3,4-methylenedioxyamphetamine (MDA), and 
      3,4-methylenedioxymethamphetamine (MDMA). Acquisition of the spectra as a 
      function of the frequency of the product ejection waveform demonstrates that the 
      signals acquired represent precursor ion scans. CONCLUSIONS: Secular frequency 
      scanning is a nonconventional method of mass scanning that in combination with 
      product ion ejection enables precursor scans in single ion traps. This phenomenon 
      is demonstrated here for a miniature linear ion trap, but the concepts described 
      also apply to quadrupole mass filters. Copyright (c) 2016 John Wiley & Sons, Ltd.
CI  - Copyright (c) 2016 John Wiley & Sons, Ltd.
FAU - Snyder, Dalton T
AU  - Snyder DT
AD  - Department of Chemistry and Center for Analytical Instrumentation Development, 
      Purdue University, West Lafayette, IN, 47907, USA.
FAU - Pulliam, Christopher J
AU  - Pulliam CJ
AD  - Department of Chemistry and Center for Analytical Instrumentation Development, 
      Purdue University, West Lafayette, IN, 47907, USA.
FAU - Cooks, R Graham
AU  - Cooks RG
AD  - Department of Chemistry and Center for Analytical Instrumentation Development, 
      Purdue University, West Lafayette, IN, 47907, USA.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
EDAT- 2016/03/13 06:00
MHDA- 2016/03/13 06:01
CRDT- 2016/03/13 06:00
PHST- 2015/11/20 00:00 [received]
PHST- 2015/12/22 00:00 [revised]
PHST- 2015/12/23 00:00 [accepted]
PHST- 2016/03/13 06:00 [entrez]
PHST- 2016/03/13 06:00 [pubmed]
PHST- 2016/03/13 06:01 [medline]
AID - 10.1002/rcm.7500 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2016 Apr 15;30(7):800-4. doi: 10.1002/rcm.7500.