PMID- 26972254
OWN - NLM
STAT- MEDLINE
DCOM- 20160906
LR  - 20160418
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 473
IP  - 2
DP  - 2016 Apr 29
TI  - Cornuside inhibits mast cell-mediated allergic response by down-regulating MAPK 
      and NF-kappaB signaling pathways.
PG  - 408-14
LID - S0006-291X(16)30320-5 [pii]
LID - 10.1016/j.bbrc.2016.03.007 [doi]
AB  - AIMS: The present study is to investigate the effect of cornuside on mast 
      cell-mediated allergic response, as well as its possible mechanisms of action. 
      METHODS: To test the anti-allergic effects of cornuside in vivo, local 
      extravasation was induced by local injection of anti-dinitrophenyl immunoglobulin 
      E (IgE) followed by intravenous antigenic challenge in passive cutaneous 
      anaphylaxis model rats. Mast cell viability was determined using MTT assay. 
      Histamine content from rat peritoneal mast cells was measured by the 
      radioenzymatic method. To investigate the mechanisms by which cornuside affects 
      the reduction of histamine release, the levels of calcium uptake were measured. 
      To examine whether cornuside affects the expression of pro-inflammatory 
      cytokines, Western blotting and ELISA were carried out. RESULTS: Oral 
      administration of cornuside inhibited passive cutaneous anaphylaxis in rats. 
      Presence of cornuside attenuated IgE-induced histamine release from rat 
      peritoneal mast cells. The inhibitory effect of cornuside on histamine release 
      was mediated by the modulation of intracellular calcium. In addition, cornuside 
      decreased phorbol 12-myristate 13-acetate (PMA) and calcium ionophore 
      A23187-stimulated production and secretion of pro-inflammatory cytokines such as 
      TNF-alpha and IL-6 in human mast cells. The inhibitory effect of cornuside on 
      pro-inflammatory cytokines was dependent on nuclear factor-kappaB and p38 
      mitogen-activated protein kinase. CONCLUSIONS: The present study provides 
      evidence that cornuside inhibits mast cell-derived inflammatory allergic 
      reactions by blocking histamine release and pro-inflammatory cytokine expression. 
      Furthermore, in vivo and in vitro anti-allergic effects of cornuside suggest a 
      possible therapeutic application of this agent in inflammatory allergic diseases.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Li, Liangchang
AU  - Li L
AD  - Department of Anatomy, Histology and Embryology, School of Basic Medical 
      Sciences, Yanbian University, Yanji, 133002, PR China.
FAU - Jin, Guangyu
AU  - Jin G
AD  - Yanbian University Hospital, Medicine College, Yanbian University, Yanji, 133000, 
      PR China.
FAU - Jiang, Jingzhi
AU  - Jiang J
AD  - Department of Anatomy, Histology and Embryology, School of Basic Medical 
      Sciences, Yanbian University, Yanji, 133002, PR China.
FAU - Zheng, Mingyu
AU  - Zheng M
AD  - College of Pharmacy, Yanbian University, Yanji, 133002, PR China.
FAU - Jin, Yan
AU  - Jin Y
AD  - College of Pharmacy, Yanbian University, Yanji, 133002, PR China.
FAU - Lin, Zhenhua
AU  - Lin Z
AD  - Department of Pathology & Cancer Research Center, Yanbian University Medical 
      College, Yanji, 133002, PR China.
FAU - Li, Guangzhao
AU  - Li G
AD  - Department of Anatomy, Histology and Embryology, School of Basic Medical 
      Sciences, Yanbian University, Yanji, 133002, PR China.
FAU - Choi, Yunho
AU  - Choi Y
AD  - Department of Anatomy, Medical School, Institute for Medical Sciences, Chonbuk 
      National University, Jeonju, 561-756, Republic of Korea. Electronic address: 
      why76@jbnu.ac.kr.
FAU - Yan, Guanghai
AU  - Yan G
AD  - Department of Anatomy, Histology and Embryology, School of Basic Medical 
      Sciences, Yanbian University, Yanji, 133002, PR China. Electronic address: 
      ghyan2015@sina.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160310
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Glucosides)
RN  - 0 (Inflammation Mediators)
RN  - 0 (NF-kappa B)
RN  - 0 (Pyrans)
RN  - 131189-57-6 (cornuside)
SB  - IM
MH  - Anaphylaxis/*drug therapy/immunology/pathology
MH  - Animals
MH  - Anti-Allergic Agents/pharmacology/*therapeutic use
MH  - Cells, Cultured
MH  - Cytokines/immunology
MH  - Drugs, Chinese Herbal/pharmacology/*therapeutic use
MH  - Glucosides/pharmacology/*therapeutic use
MH  - Histamine Release/drug effects
MH  - Inflammation Mediators/immunology
MH  - MAP Kinase Signaling System/*drug effects
MH  - Male
MH  - Mast Cells/*drug effects/immunology/pathology
MH  - Mice
MH  - NF-kappa B/*immunology
MH  - Pyrans/pharmacology/*therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Allergic response
OT  - Calcium
OT  - Histamine
OT  - Mast cell
OT  - NF-kappaB
OT  - Sesamin
EDAT- 2016/03/15 06:00
MHDA- 2016/09/07 06:00
CRDT- 2016/03/15 06:00
PHST- 2016/02/25 00:00 [received]
PHST- 2016/03/04 00:00 [accepted]
PHST- 2016/03/15 06:00 [entrez]
PHST- 2016/03/15 06:00 [pubmed]
PHST- 2016/09/07 06:00 [medline]
AID - S0006-291X(16)30320-5 [pii]
AID - 10.1016/j.bbrc.2016.03.007 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Apr 29;473(2):408-14. doi: 
      10.1016/j.bbrc.2016.03.007. Epub 2016 Mar 10.