PMID- 26972719
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 2038-8306 (Electronic)
IS  - 1121-760X (Print)
IS  - 1121-760X (Linking)
VI  - 60
IP  - 1
DP  - 2016 Feb 16
TI  - Changes in muscularis propria of anterior vaginal wall in women with pelvic organ 
      prolapse.
PG  - 2604
LID - 10.4081/ejh.2016.2604 [doi]
LID - 2604
AB  - The objective of this study was to evaluate the morphological and 
      immunohistochemical alterations of tissue removed from the upper third of 
      anterior vaginal wall in a sample group of the female population presenting 
      homogenous risk factors associated with Pelvic Organ Prolapse (POP). The case 
      study consisted of 14 patients with POP and there were 10 patients in the control 
      group. Patient selection was carried on the basis of specific criteria and all of 
      the patients involved in the study presented one or more of the recognized POP 
      risk factors. Samples were taken from POP patients during vaginal plastic surgery 
      following  colpohysterectomy, and from control patients during closure of the 
      posterior fornix following hysterectomy. Samples were processed for histological 
      and  immunohistochemical analyses for Collagen I and Collagen III, alpha-Smooth 
      Muscle Actin (alpha-SMA), Platelet-Derived-Growth-Factor (PDGF), matrix 
      metalloproteinase 3 (MMP3), Caspase3. Immunofluorescence analyses for Collagen I 
      and III and PDGF were also carried out. In prolapsed specimens our results show a 
      disorganization of smooth muscle cells that appeared to have been displaced by an 
      increased collagen III deposition resulting in rearrangement of the muscularis 
      propria architecture. These findings suggest that the increase in the expression 
      of collagen fibers in muscularis could probably due to a phenotypic switch 
      resulting in the dedifferentiation of smooth muscle cells into myofibroblasts. 
      These alterations could be responsible for the compromising of the dynamic 
      functionality of the pelvic floor.
FAU - Vetuschi, A
AU  - Vetuschi A
AD  - University of L'Aquila. antonella.vetuschi@univaq.it.
FAU - D'Alfonso, A
AU  - D'Alfonso A
FAU - Sferra, R
AU  - Sferra R
FAU - Zanelli, D
AU  - Zanelli D
FAU - Pompili, S
AU  - Pompili S
FAU - Patacchiola, F
AU  - Patacchiola F
FAU - Gaudio, E
AU  - Gaudio E
FAU - Carta, G
AU  - Carta G
LA  - eng
PT  - Journal Article
DEP - 20160216
PL  - Italy
TA  - Eur J Histochem
JT  - European journal of histochemistry : EJH
JID - 9207930
RN  - 0 (Muscle Proteins)
SB  - IM
MH  - Female
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Muscle Proteins/*biosynthesis
MH  - *Pelvic Organ Prolapse/metabolism/pathology
MH  - *Vagina/metabolism/pathology
PMC - PMC4800255
COIS- Conflict of interest: the authors declare no conflict of interest.
EDAT- 2016/03/15 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/02/16
CRDT- 2016/03/15 06:00
PHST- 2015/12/11 00:00 [received]
PHST- 2016/02/03 00:00 [accepted]
PHST- 2016/02/03 00:00 [revised]
PHST- 2016/03/15 06:00 [entrez]
PHST- 2016/03/15 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/02/16 00:00 [pmc-release]
AID - 10.4081/ejh.2016.2604 [doi]
PST - epublish
SO  - Eur J Histochem. 2016 Feb 16;60(1):2604. doi: 10.4081/ejh.2016.2604.