PMID- 26973292
OWN - NLM
STAT- MEDLINE
DCOM- 20170106
LR  - 20211203
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 427
DP  - 2016 May 15
TI  - Circulating and visceral adipose miR-100 is down-regulated in patients with 
      obesity and Type 2 diabetes.
PG  - 112-23
LID - S0303-7207(16)30058-2 [pii]
LID - 10.1016/j.mce.2016.03.010 [doi]
AB  - Obesity is a major public health problem conferring substantial excess risk for 
      Type 2 diabetes (T2D). The role of microRNAs (miRNAs) in obesity and adipose 
      tissue is not clearly defined. We hypothesize that circulating miRNA expression 
      profiles vary according to differences in body mass index (BMI) and T2D and 
      circulating miRNAs may reflect adipose tissue expression. Compared to healthy, 
      lean individuals, circulating miR-100 was significantly lower in obese 
      normoglycemic subjects and subjects with T2D. In visceral adipose tissue, 
      expression of miR-100 was lower from obese subjects with T2D compared to obese 
      subjects without T2D. miR-100 expression was significantly lower after adipogenic 
      induction in human visceral, subcutaneous adipocytes and 3T3-L1 adipocytes. 
      miR-100 reduced expression of mammalian target of rapamycin (mTOR) and Insulin 
      Growth Factor Receptor (IGFR) directly. Differentiation of 3T3-L1 was accelerated 
      by inhibition of miR-100 and reduced by miR-100 mimic transfection. Our data 
      provide the first evidence of an association of circulating miR-100 with obesity 
      and diabetes. Additionally, our in-vitro findings, and the miR-100 expression 
      patterns in site-specific adipose tissue suggest miR-100 to modulate IGFR, mTOR 
      and mediate adipogenesis.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Pek, Sharon Li Ting
AU  - Pek SL
AD  - Clinical Research Unit, Khoo Teck Puat Hospital, 768828, Singapore. Electronic 
      address: pek.sharon.lt@alexandrahealth.com.sg.
FAU - Sum, Chee Fang
AU  - Sum CF
AD  - Diabetes Centre, Khoo Teck Puat Hospital, 768828, Singapore; Division of 
      Endocrinology, Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: 
      sum.chee.fang@alexandrahealth.com.sg.
FAU - Lin, Michelle Xueqin
AU  - Lin MX
AD  - Clinical Research Unit, Khoo Teck Puat Hospital, 768828, Singapore. Electronic 
      address: lin.michelle.x@alexandrahealth.com.sg.
FAU - Cheng, Anton Kui Sing
AU  - Cheng AK
AD  - Department of General Surgery, Khoo Teck Puat Hospital, 768828, Singapore. 
      Electronic address: cheng.anton.ks@alexandrahealth.com.sg.
FAU - Wong, Michael Tack Keong
AU  - Wong MT
AD  - Health for Life, Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: 
      wong.michael.tk@alexandrahealth.com.sg.
FAU - Lim, Su Chi
AU  - Lim SC
AD  - Clinical Research Unit, Khoo Teck Puat Hospital, 768828, Singapore; Diabetes 
      Centre, Khoo Teck Puat Hospital, 768828, Singapore; Division of Endocrinology, 
      Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: 
      lim.su.chi@alexandrahealth.com.sg.
FAU - Tavintharan, Subramaniam
AU  - Tavintharan S
AD  - Clinical Research Unit, Khoo Teck Puat Hospital, 768828, Singapore; Diabetes 
      Centre, Khoo Teck Puat Hospital, 768828, Singapore; Division of Endocrinology, 
      Khoo Teck Puat Hospital, 768828, Singapore. Electronic address: 
      subramaniam.tavintharan@alexandrahealth.com.sg.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160310
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (MIRN100 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptors, Somatomedin)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - 3T3 Cells
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Cells, Cultured
MH  - Cohort Studies
MH  - Diabetes Mellitus, Type 2/complications/*metabolism
MH  - *Down-Regulation
MH  - Female
MH  - Humans
MH  - Intra-Abdominal Fat/*metabolism
MH  - Male
MH  - Mice
MH  - MicroRNAs/blood/*metabolism
MH  - Middle Aged
MH  - Obesity/complications/*metabolism
MH  - Receptors, Somatomedin/metabolism
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - Adipogenesis
OT  - IGFR
OT  - Obesity
OT  - Type 2 diabetes
OT  - mTOR
OT  - microRNAs
EDAT- 2016/03/15 06:00
MHDA- 2017/01/07 06:00
CRDT- 2016/03/15 06:00
PHST- 2015/12/02 00:00 [received]
PHST- 2016/03/04 00:00 [revised]
PHST- 2016/03/08 00:00 [accepted]
PHST- 2016/03/15 06:00 [entrez]
PHST- 2016/03/15 06:00 [pubmed]
PHST- 2017/01/07 06:00 [medline]
AID - S0303-7207(16)30058-2 [pii]
AID - 10.1016/j.mce.2016.03.010 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2016 May 15;427:112-23. doi: 10.1016/j.mce.2016.03.010. Epub 
      2016 Mar 10.