PMID- 26974138
OWN - NLM
STAT- MEDLINE
DCOM- 20170418
LR  - 20211204
IS  - 2191-0286 (Electronic)
IS  - 0792-6855 (Linking)
VI  - 27
IP  - 4
DP  - 2016 Jun 1
TI  - Role of monocyte chemoattractant protein-1, stromal derived factor-1 and retinoic 
      acid in pathophysiology of neuropathic pain in rats.
PG  - 411-24
LID - 10.1515/jbcpp-2015-0105 [doi]
AB  - BACKGROUND: Chemokines have been recently recognized to play a role in chronic 
      pain syndromes' pathophysiology. This study investigated the role of monocyte 
      chemoattractant protein-1 (MCP-1), stromal cell derived factor-1 (SDF-1), and 
      retinoic acid (RA) as targets for the therapeutic approach of neuropathic pain. 
      METHODS: A chronic constriction injury (CCI) model of neuropathic pain by 
      unilateral ligation of left sciatic nerve was performed in adult female Wistar 
      rats. The effects of doxycycline (Dox, 50 mg/kg/day i.p. for 7 days), single dose 
      of bicyclam (5 mg/kg i.p.), RA (15 mg/kg/day i.p. for 7 days), and their 
      combination(s) on behavioral tests of nociception (Von Frey filaments; paw 
      pressure test) on days 0, 1, 3, 5, and 7 of operation were studied. Serum 
      concentrations of MCP-1 and SDF-1 were measured by ELISA. Histological 
      examination of the sciatic nerve was investigated. RESULTS: CCI of sciatic nerve 
      significantly induced mechanical allodynia and hyperalgesia and an increase of 
      MCP-1 and SDF-1 serum levels. Dox-treated groups (Dox, Dox+bicyclam, Dox+RA, 
      Dox+bicyclam+RA) and bicyclam-treated groups (bicyclam, Dox+bicyclam, 
      bicyclam+RA, Dox+bicyclam+RA) attenuated CCI-induced behavioral and biochemical 
      changes. RA inhibited CCI-induced mechanical hyperalgesia but produced a 
      time-dependent reversal of allodynia. Histological findings showed degenerative 
      changes of sciatic nerve after CCI that were partially recovered in Dox-treated 
      groups. CONCLUSIONS: These findings demonstrate an association between serum 
      MCP-1 and SDF-1 concentrations and behavioral manifestations of neuropathic pain. 
      RA administration decreased neuropathic pain (antihyperalgesic effect) but did 
      not cause any improvement in sciatic nerve tissues, either alone or in 
      combination with chemokine antagonists. Thus, chemokines may serve as potential 
      targets for drug development in neuropathic pain treatment.
FAU - Hamed, Enas Ahmed
AU  - Hamed EA
FAU - Mohamed Farghaly, Hanan Sayed
AU  - Mohamed Farghaly HS
FAU - Abdel Mola, Asmaa Fathey
AU  - Abdel Mola AF
FAU - Fahmi, Minerva Kamal
AU  - Fahmi MK
FAU - Makhlouf, Madiha Mohammed
AU  - Makhlouf MM
FAU - Balfas, Mohamed Abdullah
AU  - Balfas MA
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Basic Clin Physiol Pharmacol
JT  - Journal of basic and clinical physiology and pharmacology
JID - 9101750
RN  - 0 (Benzylamines)
RN  - 0 (CXCL12 protein, rat)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cyclams)
RN  - 0 (Heterocyclic Compounds)
RN  - 5688UTC01R (Tretinoin)
RN  - N12000U13O (Doxycycline)
RN  - S915P5499N (plerixafor)
SB  - IM
MH  - Animals
MH  - Benzylamines
MH  - Chemokine CCL2/*metabolism
MH  - Chemokine CXCL12/*metabolism
MH  - Cyclams
MH  - Disease Models, Animal
MH  - Doxycycline/pharmacology
MH  - Female
MH  - Heterocyclic Compounds/pharmacology
MH  - Hyperalgesia/drug therapy/metabolism
MH  - Neuralgia/*metabolism/*pathology
MH  - Nociception/physiology
MH  - Pain Measurement/methods
MH  - Pain Threshold/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Sciatic Nerve/metabolism
MH  - Tretinoin/*metabolism
EDAT- 2016/03/15 06:00
MHDA- 2017/04/19 06:00
CRDT- 2016/03/15 06:00
PHST- 2015/08/29 00:00 [received]
PHST- 2015/12/30 00:00 [accepted]
PHST- 2016/03/15 06:00 [entrez]
PHST- 2016/03/15 06:00 [pubmed]
PHST- 2017/04/19 06:00 [medline]
AID - /j/jbcpp.ahead-of-print/jbcpp-2015-0105/jbcpp-2015-0105.xml [pii]
AID - 10.1515/jbcpp-2015-0105 [doi]
PST - ppublish
SO  - J Basic Clin Physiol Pharmacol. 2016 Jun 1;27(4):411-24. doi: 
      10.1515/jbcpp-2015-0105.