PMID- 26976361
OWN - NLM
STAT- MEDLINE
DCOM- 20161215
LR  - 20161230
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 88
IP  - 7
DP  - 2016 Apr 5
TI  - Broad-Specificity Chemiluminescence Enzyme Immunoassay for (Fluoro)quinolones: 
      Hapten Design and Molecular Modeling Study of Antibody Recognition.
PG  - 3909-16
LID - 10.1021/acs.analchem.6b00082 [doi]
AB  - On the basis of the structural features of (fluoro)quinolones (FQs), pazufloxacin 
      was first used as a generic immunizing hapten to raise a broad-specificity 
      antibody. The obtained polyclonal antibody exhibited broad cross-reactivity 
      ranging from 5.19% to 478.77% with 21 FQs. Furthermore, the antibody was able to 
      recognize these FQs below their maximum residue limits (MRLs) in an indirect 
      competitive chemiluminescence enzyme immunoassay (ic-CLEIA), with the limit of 
      detection (LOD) ranging from 0.10 to 33.83 ng/mL. For simply pretreated milk 
      samples with spiked FQs, the ic-CLEIA exhibited an excellent recovery with a 
      range of 84.6-106.9% and an acceptable coefficient of variation below 15%, 
      suggesting its suitability and reliability for the use of a promising tool to 
      detect FQs. Meanwhile, comparative molecular field analysis (CoMFA) and 
      comparative molecular similarity indices analysis (CoMSIA) models, with 
      statistically significant correlation coefficients (q(2)CoMFA = 0.559, r(2)CoMFA 
      = 0.999; q(2)CoMSIA = 0.559, r(2)CoMSIA = 0.994), were established to investigate 
      the antibody recognition mechanism. These two models revealed that in the 
      antibody, the active cavity binding FQs' 7-position substituents worked together 
      with another cavity (binding FQs' 1-position groups) to crucially endow the high 
      cross-reactivity. This investigation will be significant for better exploring the 
      recognition mechanism and for designing new haptens.
FAU - Zeng, Haopeng
AU  - Zeng H
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety/College of Food 
      Science, South China Agricultural University , Guangzhou, 510642, P. R. China.
FAU - Chen, Jiahong
AU  - Chen J
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety/College of Food 
      Science, South China Agricultural University , Guangzhou, 510642, P. R. China.
FAU - Zhang, Chijian
AU  - Zhang C
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety/College of Food 
      Science, South China Agricultural University , Guangzhou, 510642, P. R. China.
FAU - Huang, Xin-An
AU  - Huang XA
AD  - Tropical Medicine Institute & South China Chinese Medicine Collaborative 
      Innovation Center, Guangzhou University of Chinese Medicine , Guangzhou, 510405, 
      P. R. China.
FAU - Sun, Yuanming
AU  - Sun Y
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety/College of Food 
      Science, South China Agricultural University , Guangzhou, 510642, P. R. China.
FAU - Xu, Zhenlin
AU  - Xu Z
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety/College of Food 
      Science, South China Agricultural University , Guangzhou, 510642, P. R. China.
FAU - Lei, Hongtao
AU  - Lei H
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety/College of Food 
      Science, South China Agricultural University , Guangzhou, 510642, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160325
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Antibodies)
RN  - 0 (Fluoroquinolones)
RN  - 0 (Haptens)
RN  - 0 (Oxazines)
RN  - 4CZ1R38NDI (pazufloxacin)
SB  - IM
MH  - Animals
MH  - Antibodies/*immunology
MH  - Fluoroquinolones/*analysis/chemical synthesis/*immunology
MH  - Haptens/*chemistry/*immunology
MH  - Immunoenzyme Techniques/*methods
MH  - *Luminescence
MH  - Milk/chemistry
MH  - *Models, Molecular
MH  - Molecular Structure
MH  - Oxazines/*analysis/chemical synthesis/*immunology
EDAT- 2016/03/16 06:00
MHDA- 2016/12/16 06:00
CRDT- 2016/03/16 06:00
PHST- 2016/03/16 06:00 [entrez]
PHST- 2016/03/16 06:00 [pubmed]
PHST- 2016/12/16 06:00 [medline]
AID - 10.1021/acs.analchem.6b00082 [doi]
PST - ppublish
SO  - Anal Chem. 2016 Apr 5;88(7):3909-16. doi: 10.1021/acs.analchem.6b00082. Epub 2016 
      Mar 25.