PMID- 26976672
OWN - NLM
STAT- MEDLINE
DCOM- 20170119
LR  - 20220410
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Mar 15
TI  - Assessment of red blood cell deformability in type 2 diabetes mellitus and 
      diabetic retinopathy by dual optical tweezers stretching technique.
PG  - 15873
LID - 10.1038/srep15873 [doi]
LID - 15873
AB  - A pilot cross sectional study was conducted to investigate the role of red blood 
      cells (RBC) deformability in type 2 diabetes mellitus (T2DM) without and with 
      diabetic retinopathy (DR) using a dual optical tweezers stretching technique. A 
      dual optical tweezers was made by splitting and recombining a single Nd:YAG laser 
      beam. RBCs were trapped directly (i.e., without microbead handles) in the dual 
      optical tweezers where they were observed to adopt a "side-on" orientation. RBC 
      initial and final lengths after stretching were measured by digital video 
      microscopy, and a Deformability index (DI) calculated. Blood from 8 healthy 
      controls, 5 T2DM and 7 DR patients with respective mean age of 52.4 yrs, 51.6 yrs 
      and 52 yrs was analysed. Initial average length of RBCs for control group was 
      8.45 +/- 0.25 mum, 8.68 +/- 0.49 mum for DM RBCs and 8.82 +/- 0.32 mum for DR RBCs (p < 
      0.001). The DI for control group was 0.0698 +/- 0.0224, and that for DM RBCs was 
      0.0645 +/- 0.03 and 0.0635 +/- 0.028 (p < 0.001) for DR group. DI was inversely 
      related to basal length of RBCs (p =  .02). DI of RBC from DM and DR patients was 
      significantly lower in comparison with normal healthy controls. A dual optical 
      tweezers method can hence be reliably used to assess RBC deformability.
FAU - Agrawal, Rupesh
AU  - Agrawal R
AD  - Moorfields Eye Hospital NHS Foundation Trust, London, UK.
AD  - Department of Mechanical Engineering, University College London, London, UK.
AD  - Institute of Ophthalmology, University College London, London, UK.
AD  - National Healthcare Group Eye Institute, Tan Tock Seng Hospital, Singapore.
FAU - Smart, Thomas
AU  - Smart T
AD  - Department of Physics and Astronomy, University College London, London, UK.
FAU - Nobre-Cardoso, Joao
AU  - Nobre-Cardoso J
AD  - Moorfields Eye Hospital NHS Foundation Trust, London, UK.
FAU - Richards, Christopher
AU  - Richards C
AD  - Department of Physics and Astronomy, University College London, London, UK.
FAU - Bhatnagar, Rhythm
AU  - Bhatnagar R
AD  - Department of Mechanical Engineering, University College London, London, UK.
FAU - Tufail, Adnan
AU  - Tufail A
AD  - Moorfields Eye Hospital NHS Foundation Trust, London, UK.
FAU - Shima, David
AU  - Shima D
AD  - Institute of Ophthalmology, University College London, London, UK.
FAU - H Jones, Phil
AU  - H Jones P
AD  - Department of Physics and Astronomy, University College London, London, UK.
FAU - Pavesio, Carlos
AU  - Pavesio C
AD  - Moorfields Eye Hospital NHS Foundation Trust, London, UK.
AD  - Institute of Ophthalmology, University College London, London, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160315
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Cell Shape/physiology
MH  - Cross-Sectional Studies
MH  - Diabetes Mellitus, Type 2/*physiopathology
MH  - Diabetic Retinopathy/*physiopathology
MH  - Erythrocyte Deformability/*physiology
MH  - Erythrocytes/cytology
MH  - Female
MH  - Hematology/instrumentation/methods
MH  - Humans
MH  - Male
MH  - Microscopy, Video
MH  - Middle Aged
MH  - *Optical Tweezers
MH  - Pilot Projects
MH  - Regression Analysis
PMC - PMC4792142
COIS- The authors declare no competing financial interests.
EDAT- 2016/03/16 06:00
MHDA- 2017/01/20 06:00
PMCR- 2016/03/15
CRDT- 2016/03/16 06:00
PHST- 2015/05/28 00:00 [received]
PHST- 2015/09/21 00:00 [accepted]
PHST- 2016/03/16 06:00 [entrez]
PHST- 2016/03/16 06:00 [pubmed]
PHST- 2017/01/20 06:00 [medline]
PHST- 2016/03/15 00:00 [pmc-release]
AID - srep15873 [pii]
AID - 10.1038/srep15873 [doi]
PST - epublish
SO  - Sci Rep. 2016 Mar 15;6:15873. doi: 10.1038/srep15873.