PMID- 26976768 OWN - NLM STAT- MEDLINE DCOM- 20170222 LR - 20170802 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 186 DP - 2016 Jun 20 TI - The protective effect of total phenolics from Oenanthe Javanica on acute liver failure induced by D-galactosamine. PG - 53-60 LID - S0378-8741(16)30130-1 [pii] LID - 10.1016/j.jep.2016.03.024 [doi] AB - ETHNOPHARMACOLOGY RELEVANCE: Water dropwort [Oenanthe javanica (O. javanica)] is an aquatic perennial herb cultivated in East Asian countries. It has been popularly used in traditional Chinese medicine which is beneficial for the treatment of many diseases, including jaundice and various types of chronic and acute hepatitis. In the present study, we investigated the hepatoprotective effect of total phenolics from O. javanica (TPOJ) against D-galactosamine (D-GalN) induced liver injury in mice. MATERIAL AND METHODS: The hepatoprotective activity of TPOJ (125, 250 and 500mg/kg) was investigated on D-GalN (800mg/kg)-induced liver damages in mice. Blood and liver were collected for biochemical and microscopic analysis. RT-PCR was used to determine the changes in hepatic nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Protein levels of iNOS, COX-2, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were determined by western blotting. RESULTS: In the animal studies, TPOJ could improve the survival of acute liver failure model significantly and prevente the D-GalN-induced elevation of the serum enzymatic markers and nonenzymatic markers levels significantly. Meanwhile, TPOJ-treatment decreased the malondialdehyde (MDA) level and elevated the content of glutathione (GSH) in the liver as compared to those in the D-GalN group. Hepatic activities and protein expressions of antioxidative enzymes, including SOD, GPx, and CAT were enhanced dose dependently with TPOJ. At the same time, application of TPOJ effectively suppressed the D-GalN-induced proinflammatory mRNA and protein expression of iNOS and COX-2. Subsequently, the serum levels of proinflammatory mediators, nitric oxide (NO) and prostaglandin E2 (PGE2) were reduced. Additionally, histological analyses also showed that TPOJ reduced the extent of liver lesions induced by D-GalN. CONCLUSION: Our investigation demonstrated the hepatoprotective activity of TPOJ and revealed that TPOJ attributed its significance in the traditional use for treating liver diseases. CI - Copyright (c) 2016. Published by Elsevier Ireland Ltd. FAU - Ai, Guo AU - Ai G AD - Hospital of Institute of Aviation Medicine of Air Force, Beijing 100142, China; Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. Electronic address: guoair@163.com. FAU - Huang, Zheng-Ming AU - Huang ZM AD - Department of Pharmacy, 302 Hospital of PLA, Beijing 100039, China. Electronic address: huangzhengming@cmea.org.cn. FAU - Liu, Qing-Chuan AU - Liu QC AD - Department of Pharmacy, 302 Hospital of PLA, Beijing 100039, China. Electronic address: frog207@163.com. FAU - Han, Yan-Quan AU - Han YQ AD - Department of Pharmacy, 302 Hospital of PLA, Beijing 100039, China. Electronic address: YHS786@139.com. FAU - Chen, Xi AU - Chen X AD - 458 Hospital of PLA, Guangzhou 510062, China. Electronic address: ywc458@163.com. LA - eng PT - Journal Article DEP - 20160311 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Phenols) RN - 0 (Plant Extracts) RN - 0 (Silymarin) RN - 7535-00-4 (Galactosamine) SB - IM MH - Animals MH - Dose-Response Relationship, Drug MH - Galactosamine/*toxicity MH - Liver Failure, Acute/*chemically induced/*drug therapy MH - Male MH - Mice MH - Oenanthe/*chemistry MH - Oxidative Stress/drug effects MH - Phenols/administration & dosage/chemistry/*pharmacology MH - Phytotherapy MH - Plant Extracts/administration & dosage/chemistry/*pharmacology MH - Silymarin/administration & dosage/pharmacology OTO - NOTNLM OT - D-Galactosamine OT - Hepatoprotective effect OT - Oenanthe Javanica OT - Total phenolics EDAT- 2016/03/16 06:00 MHDA- 2017/02/23 06:00 CRDT- 2016/03/16 06:00 PHST- 2015/07/02 00:00 [received] PHST- 2016/02/17 00:00 [revised] PHST- 2016/03/11 00:00 [accepted] PHST- 2016/03/16 06:00 [entrez] PHST- 2016/03/16 06:00 [pubmed] PHST- 2017/02/23 06:00 [medline] AID - S0378-8741(16)30130-1 [pii] AID - 10.1016/j.jep.2016.03.024 [doi] PST - ppublish SO - J Ethnopharmacol. 2016 Jun 20;186:53-60. doi: 10.1016/j.jep.2016.03.024. Epub 2016 Mar 11.