PMID- 26979838
OWN - NLM
STAT- MEDLINE
DCOM- 20170810
LR  - 20181113
IS  - 1432-184X (Electronic)
IS  - 0095-3628 (Linking)
VI  - 72
IP  - 1
DP  - 2016 Jul
TI  - Effects of Hypoxia on the Phylogenetic Composition and Species Distribution of 
      Protists in a Subtropical Harbor.
PG  - 96-105
LID - 10.1007/s00248-016-0751-7 [doi]
AB  - Tolo Harbor, a subtropical semi-enclosed coastal water body, is surrounded by an 
      expanding urban community, which contributes to large concentrations of nutrient 
      runoff, leading to algal blooms and localized hypoxic episodes. Present knowledge 
      of protist distributions in subtropical waters during hypoxic conditions is very 
      limited. In this study, therefore, we combined parallel 454 pyrosequencing 
      technology and denaturing gradient gel electrophoresis (DGGE) fingerprint 
      analyses to reveal the protist community shifts before, during, and after a 
      2-week hypoxic episode during the summer of 2011. Hierarchical clustering for 
      DGGE demonstrated similar grouping of hypoxic samples separately from oxic 
      samples. Dissolved oxygen (DO) concentration and dissolved inorganic 
      nitrogen:phosphate (DIN:PO4) concentrations significantly affected OTU 
      distribution in 454 sequenced samples, and a shift toward a ciliate and marine 
      alveolate clade II (MALV II) species composition occurred as waters shifted from 
      oxic to hypoxic. These results suggest that protist community shifts toward 
      heterotrophic and parasitic tendencies as well as decreased diversity and 
      richness in response to hypoxic outbreaks.
FAU - Rocke, Emma
AU  - Rocke E
AD  - Division of Life Science, Hong Kong University of Science and Technology, Clear 
      Water Bay, Kowloon, Hong Kong.
FAU - Jing, Hongmei
AU  - Jing H
AD  - Division of Life Science, Hong Kong University of Science and Technology, Clear 
      Water Bay, Kowloon, Hong Kong.
AD  - Sanya Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, 
      Sanya, 572000, China.
FAU - Xia, Xiaomin
AU  - Xia X
AD  - Division of Life Science, Hong Kong University of Science and Technology, Clear 
      Water Bay, Kowloon, Hong Kong.
FAU - Liu, Hongbin
AU  - Liu H
AD  - Division of Life Science, Hong Kong University of Science and Technology, Clear 
      Water Bay, Kowloon, Hong Kong. liuhb@ust.hk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160315
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (Phosphates)
RN  - N762921K75 (Nitrogen)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - *Biodiversity
MH  - Biofilms
MH  - Ciliophora/*classification/isolation & purification
MH  - Cluster Analysis
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Eukaryota/*classification/isolation & purification
MH  - Hong Kong
MH  - Hypoxia/*metabolism
MH  - Nitrogen/analysis
MH  - Oxygen/analysis
MH  - Phosphates/analysis
MH  - *Phylogeny
MH  - Seawater/chemistry/microbiology
MH  - Sequence Analysis, DNA
MH  - Species Specificity
OTO - NOTNLM
OT  - DGGE
OT  - Hypoxia
OT  - Protists
OT  - Pyrosequencing
EDAT- 2016/03/17 06:00
MHDA- 2017/08/11 06:00
CRDT- 2016/03/17 06:00
PHST- 2015/03/06 00:00 [received]
PHST- 2016/03/02 00:00 [accepted]
PHST- 2016/03/17 06:00 [entrez]
PHST- 2016/03/17 06:00 [pubmed]
PHST- 2017/08/11 06:00 [medline]
AID - 10.1007/s00248-016-0751-7 [pii]
AID - 10.1007/s00248-016-0751-7 [doi]
PST - ppublish
SO  - Microb Ecol. 2016 Jul;72(1):96-105. doi: 10.1007/s00248-016-0751-7. Epub 2016 Mar 
      15.