PMID- 26983247
OWN - NLM
STAT- MEDLINE
DCOM- 20160525
LR  - 20181202
IS  - 1001-4454 (Print)
IS  - 1001-4454 (Linking)
VI  - 38
IP  - 8
DP  - 2015 Aug
TI  - [Protective Effects of Ginkgolide N Against Glutamate-Induced Injury in PC12 
      Cells].
PG  - 1694-8
AB  - OBJECTIVE: To study the protective effects of ginkgolide N against 
      glutamte-induced injury in PC12 cells and its mechanisms. METHODS: The injury 
      model was established by treating PC12 cells with glutamate, and PC12 cells were 
      treated with different concentrations of ginkgolide N with ginkgolide B as 
      control. The cells activity was analyzed by MTT assay. The apoptosis of PC12 
      cells were examined by acridine orange( AO) staining, the reactive oxygen species 
      and mitochondrial membrane potential of PC12 cells were examined by flow 
      cytometry. Western blot method was used to examine the expression of Cleaved 
      Caspase-3 protein. RESULTS: Ginkgolides N of 2-8 microgmol/L inhibited PC12 cells 
      apoptosis and ROS accumulation induced by glutamate,stabilized membrane potential 
      of damaged PC12, and reduced the expression of Cleaved Caspase-3 protein. 
      CONCLUSION: Ginkgolide N has a protective effect on PC12 cells injury induced by 
      glutamate, and the mechanism may be associated with reducing ROS generation, 
      stabilizing membrane potential and inhibiting the expression of Cleaved Caspase-3 
      protein.
FAU - Zhao, Zhen-dong
AU  - Zhao ZD
FAU - Zhang, Lei-hong
AU  - Zhang LH
FAU - Xia, Li
AU  - Xia L
FAU - Zhang, Xian-tao
AU  - Zhang XT
FAU - Fang, Chun-sheng
AU  - Fang CS
FAU - Ma, Jian-rongi
AU  - Ma JR
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhong Yao Cai
JT  - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
JID - 9426370
RN  - 0 (Ginkgolides)
RN  - 0 (Protective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Caspase 3/metabolism
MH  - Ginkgolides/*pharmacology
MH  - Glutamic Acid/*adverse effects
MH  - *Membrane Potential, Mitochondrial
MH  - PC12 Cells/drug effects
MH  - Protective Agents/*pharmacology
MH  - Rats
MH  - Reactive Oxygen Species/*metabolism
EDAT- 2016/03/18 06:00
MHDA- 2016/05/26 06:00
CRDT- 2016/03/18 06:00
PHST- 2016/03/18 06:00 [entrez]
PHST- 2016/03/18 06:00 [pubmed]
PHST- 2016/05/26 06:00 [medline]
PST - ppublish
SO  - Zhong Yao Cai. 2015 Aug;38(8):1694-8.