PMID- 26984058
OWN - NLM
STAT- MEDLINE
DCOM- 20170223
LR  - 20181202
IS  - 1943-2631 (Electronic)
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 203
IP  - 1
DP  - 2016 May
TI  - Heterochromatin-Associated Proteins HP1a and Piwi Collaborate to Maintain the 
      Association of Achiasmate Homologs in Drosophila Oocytes.
PG  - 173-89
LID - 10.1534/genetics.115.186460 [doi]
AB  - Accurate segregation of homologous chromosomes during meiosis depends on their 
      ability to remain physically connected throughout prophase I. For homologs that 
      achieve a crossover, sister chromatid cohesion distal to the chiasma keeps them 
      attached until anaphase I. However, in Drosophila melanogaster wild-type oocytes, 
      chromosome 4 never recombines, and the X chromosome fails to cross over in 6-10% 
      of oocytes. Proper segregation of these achiasmate homologs relies on their 
      pericentric heterochromatin-mediated association, but the mechanism(s) underlying 
      this attachment remains poorly understood. Using an inducible RNA interference 
      (RNAi) strategy combined with fluorescence in situ hybridization (FISH) to 
      monitor centromere proximal association of the achiasmate FM7a/X homolog pair, we 
      asked whether specific heterochromatin-associated proteins are required for the 
      association and proper segregation of achiasmate homologs in Drosophila oocytes. 
      When we knock down HP1a, H3K9 methytransferases, or the HP1a binding partner Piwi 
      during mid-prophase, we observe significant disruption of pericentric 
      heterochromatin-mediated association of FM7a/X homologs. Furthermore, for both 
      HP1a and Piwi knockdown oocytes, transgenic coexpression of the corresponding 
      wild-type protein is able to rescue RNAi-induced defects, but expression of a 
      mutant protein with a single amino acid change that disrupts the HP1a-Piwi 
      interaction is unable to do so. We show that Piwi is stably bound to numerous 
      sites along the meiotic chromosomes, including centromere proximal regions. In 
      addition, reduction of HP1a or Piwi during meiotic prophase induces a significant 
      increase in FM7a/X segregation errors. We present a speculative model outlining 
      how HP1a and Piwi could collaborate to keep achiasmate chromosomes associated in 
      a homology-dependent manner.
CI  - Copyright (c) 2016 by the Genetics Society of America.
FAU - Giauque, Christopher C
AU  - Giauque CC
AD  - Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 
      03755.
FAU - Bickel, Sharon E
AU  - Bickel SE
AD  - Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 
      03755 sharon.e.bickel@dartmouth.edu.
LA  - eng
GR  - P40 OD010949/OD/NIH HHS/United States
GR  - P40 OD018537/OD/NIH HHS/United States
GR  - R01 GM059354/GM/NIGMS NIH HHS/United States
GR  - R01 GM084947/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160316
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Argonaute Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Histones)
RN  - 0 (heterochromatin protein 1, Drosophila)
RN  - 0 (piwi protein, Drosophila)
RN  - EC 2.1.1.- (Histone Methyltransferases)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Animals
MH  - Argonaute Proteins/*metabolism
MH  - Chromosomal Proteins, Non-Histone/genetics/*metabolism
MH  - Chromosome Segregation
MH  - DNA-Binding Proteins
MH  - Drosophila/*genetics/*metabolism
MH  - Drosophila Proteins/genetics/*metabolism
MH  - Female
MH  - Gene Knockdown Techniques
MH  - Histone Methyltransferases
MH  - Histone-Lysine N-Methyltransferase/metabolism
MH  - Histones/metabolism
MH  - Meiosis
MH  - Mutation
MH  - Nondisjunction, Genetic
MH  - Oocytes/*metabolism
MH  - Protein Binding
PMC - PMC4858772
OTO - NOTNLM
OT  - *H3K9 methyltransferases
OT  - *chromosome segregation
OT  - *meiosis
OT  - *nondisjunction
OT  - *pericentric heterochromatin
EDAT- 2016/03/18 06:00
MHDA- 2017/02/24 06:00
PMCR- 2017/05/01
CRDT- 2016/03/18 06:00
PHST- 2015/12/22 00:00 [received]
PHST- 2016/03/11 00:00 [accepted]
PHST- 2016/03/18 06:00 [entrez]
PHST- 2016/03/18 06:00 [pubmed]
PHST- 2017/02/24 06:00 [medline]
PHST- 2017/05/01 00:00 [pmc-release]
AID - genetics.115.186460 [pii]
AID - 186460 [pii]
AID - 10.1534/genetics.115.186460 [doi]
PST - ppublish
SO  - Genetics. 2016 May;203(1):173-89. doi: 10.1534/genetics.115.186460. Epub 2016 Mar 
      16.