PMID- 26984998
OWN - NLM
STAT- MEDLINE
DCOM- 20170207
LR  - 20170207
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 94
IP  - 4
DP  - 2016 Apr
TI  - Energy Utilization for Survival and Fertilization-Parsimonious Quiescent Sperm 
      Turn Extravagant on Motility Activation in Rat.
PG  - 96
LID - 10.1095/biolreprod.115.137752 [doi]
AB  - Quiescent sperm survive in cauda epididymis for long periods of time under 
      extreme crowding conditions and with a very limited energy substrate, while after 
      ejaculation, motile sperm live for a much shorter period with an unlimited energy 
      resource and without crowding. Thus, the energy metabolism in relation to the 
      energy requirement of the two may be quite different. A simple physiological 
      technique was evolved to collect viable quiescent sperm from rat cauda epididymis 
      to compare its energy metabolism with motile sperm. Quiescent sperm exhibited 
      40%-60% higher activities of mitochondrial electron transport chain complexes 
      I-IV and ATP synthase in comparison to motile sperm and accumulated Ca(2+) in the 
      midpiece mitochondria to enhance oxidative phosphorylation (OxPhos). In contrast, 
      motile sperm displayed up to 75% higher activities of key glycolytic enzymes and 
      secreted more than two times the lactate than quiescent sperm. Quiescent sperm 
      phosphorylated AMPK and MAPK-p38, while motile sperm phosphorylated AKT and 
      MAPK/ERK. Glycolytic inhibitor iodoacetamide prevented motility activation of 
      quiescent rat sperm and inhibited conception in rabbits more effectively than 
      OxPhos uncoupler 2,4-dinitrophenol. Apparently, quiescent sperm employ the most 
      energy efficient OxPhos to survive for extended periods of time under extreme 
      conditions of nutrition and crowding. However, on motility initiation, sperm 
      switch predominantly to glycolysis to cater to their high- and quick-energy 
      requirement of much shorter periods. This study also presents a proof of concept 
      for targeting sperm energy metabolism for contraception.
CI  - (c) 2016 by the Society for the Study of Reproduction, Inc.
FAU - Kumar, Lokesh
AU  - Kumar L
AD  - Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India.
FAU - Yadav, Santosh K
AU  - Yadav SK
AD  - Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India.
FAU - Kushwaha, Bhavana
AU  - Kushwaha B
AD  - Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India.
FAU - Pandey, Aastha
AU  - Pandey A
AD  - Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India.
FAU - Sharma, Vikas
AU  - Sharma V
AD  - Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India.
FAU - Verma, Vikas
AU  - Verma V
AD  - Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India.
FAU - Maikhuri, Jagdamba P
AU  - Maikhuri JP
AD  - Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India.
FAU - Rajender, Singh
AU  - Rajender S
AD  - Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India.
FAU - Sharma, Vishnu L
AU  - Sharma VL
AD  - Division of Medicinal & Process Chemistry, CSIR-Central Drug Research Institute, 
      Lucknow, India.
FAU - Gupta, Gopal
AU  - Gupta G
AD  - Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India 
      g_gupta@cdri.res.in.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160316
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 33X04XA5AT (Lactic Acid)
RN  - EC 3.6.3.- (Mitochondrial Proton-Translocating ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - *Energy Metabolism
MH  - Female
MH  - Fertility
MH  - Glycolysis
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Lactic Acid/metabolism
MH  - MAP Kinase Signaling System
MH  - Male
MH  - Mitochondria/metabolism
MH  - Mitochondrial Proton-Translocating ATPases/metabolism
MH  - Rabbits
MH  - Rats, Sprague-Dawley
MH  - *Sperm Motility
OTO - NOTNLM
OT  - epididymis
OT  - gamete biology
OT  - metabolism
OT  - sperm
OT  - sperm motility and transport
EDAT- 2016/03/18 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/03/18 06:00
PHST- 2015/12/12 00:00 [received]
PHST- 2016/03/08 00:00 [accepted]
PHST- 2016/03/18 06:00 [entrez]
PHST- 2016/03/18 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
AID - biolreprod.115.137752 [pii]
AID - 10.1095/biolreprod.115.137752 [doi]
PST - ppublish
SO  - Biol Reprod. 2016 Apr;94(4):96. doi: 10.1095/biolreprod.115.137752. Epub 2016 Mar 
      16.