PMID- 26985867
OWN - NLM
STAT- MEDLINE
DCOM- 20161226
LR  - 20171116
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 37
IP  - 5
DP  - 2016 May
TI  - Upregulation of miRNA-155 expression by OxLDL in dendritic cells involves JAK1/2 
      kinase and transcription factors YY1 and MYB.
PG  - 1371-8
LID - 10.3892/ijmm.2016.2526 [doi]
AB  - Dendritic cells (DCs) have been implicated in the pathogenesis of atherosclerosis 
      (AS). Whereas the pathogenic role of oxidized low-density lipoprotein (oxLDL) in 
      the development and progression of AS has been recognized previously, the 
      contribution of microRNA-155 (miR-155) to AS was previously not fully understood. 
      It had also been noted that miR-155 levels were upregulated by oxLDL in various 
      cell types under different (patho)physiological conditions, but its underlying 
      mechanisms had not been examiend in detail. Thus, in the present study, we 
      observed that oxLDL treatment increased miR-155 expression in DCs, and 
      transfecting DCs with siRNA against scavenger receptor A (SRA) revealed that 
      repression of SRA attenuated this upregulation. We also noted that miR-155 
      negatively regulated SRA expression by suppressing the JNK pathway. Furthermore, 
      we noted that Yin Yang 1 (YY1) and V-Myb avian myeloblastosis viral oncogene 
      homolog (MYB), which were also upregulated by oxLDL, directly bound to the 
      cognate sequences of the promoter region of miR-155 to activate its 
      transcription. In addition, using SP600125, a specific inhibitor for c-Jun 
      N-terminal kinase (JNK) signaling, we demonstrated that JNK signaling was 
      involved in the miR‑155-mediated suppression of SRA expression. Thus, in the 
      present study we uncovered the molecular mechanism through which miR-155 
      expression is regulated by oxLDL, and we also identified a negative feedback 
      loop, miR‑155-JNK-SRA-miR-155. Our findings thus provide novel insights into the 
      regulatory network underlying the expression and activity of miR-155 in DCs.
FAU - Yan, Hui
AU  - Yan H
AD  - Department of Cardiology, The First Af fi liated Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Wang, Shuai
AU  - Wang S
AD  - Department of Cardiology, The First Af fi liated Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Li, Zhenwei
AU  - Li Z
AD  - Department of Cardiology, The First Af fi liated Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Zhao, Wenting
AU  - Zhao W
AD  - Department of Cardiology, The First Af fi liated Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Wang, Zhen
AU  - Wang Z
AD  - Department of Cardiology, The First Af fi liated Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Sun, Zewei
AU  - Sun Z
AD  - Department of Cardiology, The First Af fi liated Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Pan, Yanyun
AU  - Pan Y
AD  - Department of Cardiology, The First Af fi liated Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
FAU - Zhu, Jianhua
AU  - Zhu J
AD  - Department of Cardiology, The First Af fi liated Hospital, School of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160311
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (CD36 Antigens)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (MIRN155 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins c-myb)
RN  - 0 (YY1 Transcription Factor)
RN  - 0 (oxidized low density lipoprotein)
RN  - EC 2.7.10.2 (Janus Kinase 1)
RN  - EC 2.7.10.2 (Janus Kinase 2)
SB  - IM
MH  - CD36 Antigens/metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/*metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Janus Kinase 1/*metabolism
MH  - Janus Kinase 2/*metabolism
MH  - Lipoproteins, LDL/*metabolism
MH  - MicroRNAs/*genetics
MH  - Models, Biological
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Proto-Oncogene Proteins c-myb/*metabolism
MH  - *Signal Transduction
MH  - Transcription, Genetic
MH  - Up-Regulation
MH  - YY1 Transcription Factor/*metabolism
EDAT- 2016/03/18 06:00
MHDA- 2016/12/27 06:00
CRDT- 2016/03/18 06:00
PHST- 2015/07/28 00:00 [received]
PHST- 2016/03/03 00:00 [accepted]
PHST- 2016/03/18 06:00 [entrez]
PHST- 2016/03/18 06:00 [pubmed]
PHST- 2016/12/27 06:00 [medline]
AID - 10.3892/ijmm.2016.2526 [doi]
PST - ppublish
SO  - Int J Mol Med. 2016 May;37(5):1371-8. doi: 10.3892/ijmm.2016.2526. Epub 2016 Mar 
      11.