PMID- 26987686
OWN - NLM
STAT- MEDLINE
DCOM- 20171201
LR  - 20181113
IS  - 1440-1711 (Electronic)
IS  - 0818-9641 (Linking)
VI  - 94
IP  - 7
DP  - 2016 Aug
TI  - Increased expression with differential subcellular location of cytidine deaminase 
      APOBEC3G in human CD4(+) T-cell activation and dendritic cell maturation.
PG  - 689-700
LID - 10.1038/icb.2016.28 [doi]
AB  - APOBEC3G (apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G; 
      A3G) is an innate defense protein showing activity against retroviruses and 
      retrotransposons. Activated CD4(+) T cells are highly permissive for HIV-1 
      replication, whereas resting CD4(+) T cells are refractory. Dendritic cells 
      (DCs), especially mature DCs, are also refractory. We investigated whether these 
      differences could be related to a differential A3G expression and/or subcellular 
      distribution. We found that A3G mRNA and protein expression is very low in 
      resting CD4(+) T cells and immature DCs, but increases strongly following T-cell 
      activation and DC maturation. The Apo-7 anti-A3G monoclonal antibody (mAb), which 
      was specifically developed, confirmed these differences at the protein level and 
      disclosed that A3G is mainly cytoplasmic in resting CD4(+) T cells and immature 
      DCs. Nevertheless, A3G translocates to the nucleus in activated-proliferating 
      CD4(+) T cells, yet remaining cytoplasmic in matured DCs, a finding confirmed by 
      immunoblotting analysis of cytoplasmic and nuclear fractions. Apo-7 mAb was able 
      to immunoprecipitate endogenous A3G allowing to detect complexes with numerous 
      proteins in activated-proliferating but not in resting CD4(+) T cells. The 
      results show for the first time the nuclear translocation of A3G in 
      activated-proliferating CD4(+) T cells.
FAU - Oliva, Harold
AU  - Oliva H
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS)-AIDS Research 
      Group and HIV Vaccine Development in Catalonia (HIVACAT), Hospital Clinic de 
      Barcelona, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
FAU - Pacheco, Rodrigo
AU  - Pacheco R
AD  - Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad 
      Andres Bello, Santiago, Chile.
AD  - Laboratorio de Neuroinmunologia, Fundacion Ciencia and Vida, Santiago, Chile.
FAU - Martinez-Navio, Jose M
AU  - Martinez-Navio JM
AD  - Department of Biochemistry and Molecular Biology, Faculty of Biology, University 
      of Barcelona, Barcelona, Spain.
FAU - Rodriguez-Garcia, Marta
AU  - Rodriguez-Garcia M
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS)-AIDS Research 
      Group and HIV Vaccine Development in Catalonia (HIVACAT), Hospital Clinic de 
      Barcelona, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
AD  - Service of Immunology, Hospital Clinic Universitari de Barcelona, Barcelona, 
      Spain.
FAU - Naranjo-Gomez, Mar
AU  - Naranjo-Gomez M
AD  - LIRAD (Laboratory of Immunobiology for Research and Diagnostic Applications), 
      Institut d'Investigacio Germans Trias-Pujol, Autonomous University of Barcelona, 
      Badalona (Barcelona), Spain.
FAU - Climent, Nuria
AU  - Climent N
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS)-AIDS Research 
      Group and HIV Vaccine Development in Catalonia (HIVACAT), Hospital Clinic de 
      Barcelona, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
FAU - Prado, Carolina
AU  - Prado C
AD  - Laboratorio de Neuroinmunologia, Fundacion Ciencia and Vida, Santiago, Chile.
FAU - Gil, Cristina
AU  - Gil C
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS)-AIDS Research 
      Group and HIV Vaccine Development in Catalonia (HIVACAT), Hospital Clinic de 
      Barcelona, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
FAU - Plana, Montserrat
AU  - Plana M
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS)-AIDS Research 
      Group and HIV Vaccine Development in Catalonia (HIVACAT), Hospital Clinic de 
      Barcelona, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
FAU - Garcia, Felipe
AU  - Garcia F
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS)-AIDS Research 
      Group and HIV Vaccine Development in Catalonia (HIVACAT), Hospital Clinic de 
      Barcelona, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
AD  - Service of Infectious Diseases and AIDS Unit, Hospital Clinic de Barcelona, 
      Barcelona, Spain.
FAU - Miro, Jose M
AU  - Miro JM
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS)-AIDS Research 
      Group and HIV Vaccine Development in Catalonia (HIVACAT), Hospital Clinic de 
      Barcelona, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
AD  - Service of Infectious Diseases and AIDS Unit, Hospital Clinic de Barcelona, 
      Barcelona, Spain.
FAU - Franco, Rafael
AU  - Franco R
AD  - Department of Biochemistry and Molecular Biology, Faculty of Biology, University 
      of Barcelona, Barcelona, Spain.
AD  - CIBERNED Centro de Investigacion en Red, Enfermedades Neurodegenerativas, 
      Instituto de Salud Carlos III, Madrid, Spain.
FAU - Borras, Francesc E
AU  - Borras FE
AD  - IVECAT-Group, Institut d'Investigacio Germans Trias i Pujol (IGTP), Badalona, 
      Spain.
AD  - Nephrology Service, Germans Trias i Pujol University Hospital, Badalona, Spain.
FAU - Navaratnam, Naveenan
AU  - Navaratnam N
AD  - MRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital 
      Campus, London, UK.
FAU - Gatell, Jose M
AU  - Gatell JM
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS)-AIDS Research 
      Group and HIV Vaccine Development in Catalonia (HIVACAT), Hospital Clinic de 
      Barcelona, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
AD  - Service of Infectious Diseases and AIDS Unit, Hospital Clinic de Barcelona, 
      Barcelona, Spain.
FAU - Gallart, Teresa
AU  - Gallart T
AD  - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS)-AIDS Research 
      Group and HIV Vaccine Development in Catalonia (HIVACAT), Hospital Clinic de 
      Barcelona, Faculty of Medicine, University of Barcelona, Barcelona, Spain.
AD  - Service of Immunology, Hospital Clinic Universitari de Barcelona, Barcelona, 
      Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160318
PL  - United States
TA  - Immunol Cell Biol
JT  - Immunology and cell biology
JID - 8706300
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (RNA, Messenger)
RN  - EC 3.5.4.5 (APOBEC-3G Deaminase)
RN  - EC 3.5.4.5 (APOBEC3G protein, human)
SB  - IM
MH  - APOBEC-3G Deaminase/genetics/immunology/*metabolism
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - *Cell Differentiation
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Dendritic Cells/*cytology
MH  - Humans
MH  - Immunoprecipitation
MH  - Lymphocyte Activation/*immunology
MH  - Mice, Inbred BALB C
MH  - Molecular Weight
MH  - Monocytes/cytology
MH  - Protein Binding
MH  - RNA, Messenger/genetics/metabolism
MH  - Subcellular Fractions/enzymology
MH  - Up-Regulation/genetics
EDAT- 2016/03/19 06:00
MHDA- 2017/12/02 06:00
CRDT- 2016/03/19 06:00
PHST- 2015/07/12 00:00 [received]
PHST- 2016/03/09 00:00 [revised]
PHST- 2016/03/13 00:00 [accepted]
PHST- 2016/03/19 06:00 [entrez]
PHST- 2016/03/19 06:00 [pubmed]
PHST- 2017/12/02 06:00 [medline]
AID - icb201628 [pii]
AID - 10.1038/icb.2016.28 [doi]
PST - ppublish
SO  - Immunol Cell Biol. 2016 Aug;94(7):689-700. doi: 10.1038/icb.2016.28. Epub 2016 
      Mar 18.