PMID- 26987844
OWN - NLM
STAT- MEDLINE
DCOM- 20171016
LR  - 20171113
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 173
DP  - 2016 May
TI  - Pro-lymphangiogenic properties of IFN-gamma-activated human dendritic cells.
PG  - 26-35
LID - S0165-2478(16)30033-5 [pii]
LID - 10.1016/j.imlet.2016.03.008 [doi]
AB  - Dendritic cells (DCs) play a crucial role in the initiation of adaptive immune 
      responses. In addition, through the release of pro- and anti-angiogenic 
      mediators, DCs are key regulators of blood vessel remodeling, a process that 
      characterizes inflammation. Less information is available on the role of DCs in 
      lymphangiogenesis. This study reports that human DCs produce VEGF-C, a cytokine 
      with potent pro-lymphangiogenic activity when stimulated with IFN-gamma. DC-derived 
      VEGF-C was biologically active, being able to promote tube-like structure 
      formation in cultures of human lymphatic endothelial cells (LECs). DCs 
      co-cultured with IL-15-activated NK cells produced high levels of VEGF-C, 
      suggesting a role for NK-DC cross-talk in peripheral lymphoid and non-lymphoid 
      tissues in inflammation-associated lymphangiogenesis. Induction of VEGF-C by 
      IFN-gamma was detected also in other myeloid cells, such as blood-purified CD1c(+) 
      DCs, CD14(+) monocytes and in monocyte-derived macrophages. In all these cell 
      types, VEGF-C was found associated with the cell membrane by low affinity, 
      heparan sulphate-mediated, interactions. Therefore, human DCs should be 
      considered as new players in inflammation-associated lymphangiogenesis.
CI  - Copyright (c) 2016 European Federation of Immunological Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Gagliostro, Vincenzo
AU  - Gagliostro V
AD  - Department of Molecular and Translational Medicine, University of Brescia, 
      Brescia, Italy.
FAU - Seeger, Pascal
AU  - Seeger P
AD  - Department of Molecular and Translational Medicine, University of Brescia, 
      Brescia, Italy.
FAU - Garrafa, Emirena
AU  - Garrafa E
AD  - Department of Molecular and Translational Medicine, University of Brescia, 
      Brescia, Italy.
FAU - Salvi, Valentina
AU  - Salvi V
AD  - Department of Molecular and Translational Medicine, University of Brescia, 
      Brescia, Italy.
FAU - Bresciani, Roberto
AU  - Bresciani R
AD  - Department of Molecular and Translational Medicine, University of Brescia, 
      Brescia, Italy.
FAU - Bosisio, Daniela
AU  - Bosisio D
AD  - Department of Molecular and Translational Medicine, University of Brescia, 
      Brescia, Italy.
FAU - Sozzani, Silvano
AU  - Sozzani S
AD  - Department of Molecular and Translational Medicine, University of Brescia, 
      Brescia, Italy; Humanitas Clinical Research Center, Rozzano, Italy. Electronic 
      address: silvano.sozzani@unibs.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160315
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Interleukin-15)
RN  - 0 (Vascular Endothelial Growth Factor C)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cell Communication
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*physiology
MH  - Endothelium, Lymphatic/*physiology
MH  - Humans
MH  - Inflammation/*immunology
MH  - Interferon-gamma/immunology
MH  - Interleukin-15/metabolism
MH  - Killer Cells, Natural/*immunology
MH  - *Lymphangiogenesis
MH  - Lymphatic Vessels/*physiology
MH  - Macrophages/physiology
MH  - Monocytes/physiology
MH  - Vascular Endothelial Growth Factor C/*metabolism
OTO - NOTNLM
OT  - Angiogenesis
OT  - Cytokines
OT  - LEC
OT  - NK cells
OT  - VEGF-C
EDAT- 2016/03/19 06:00
MHDA- 2017/10/17 06:00
CRDT- 2016/03/19 06:00
PHST- 2016/03/07 00:00 [received]
PHST- 2016/03/11 00:00 [accepted]
PHST- 2016/03/19 06:00 [entrez]
PHST- 2016/03/19 06:00 [pubmed]
PHST- 2017/10/17 06:00 [medline]
AID - S0165-2478(16)30033-5 [pii]
AID - 10.1016/j.imlet.2016.03.008 [doi]
PST - ppublish
SO  - Immunol Lett. 2016 May;173:26-35. doi: 10.1016/j.imlet.2016.03.008. Epub 2016 Mar 
      15.