PMID- 26988085
OWN - NLM
STAT- MEDLINE
DCOM- 20161012
LR  - 20240325
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 47
DP  - 2016 Mar 17
TI  - Comparative analysis of routes of immunization of a live porcine reproductive and 
      respiratory syndrome virus (PRRSV) vaccine in a heterologous virus challenge 
      study.
PG  - 45
LID - 10.1186/s13567-016-0331-3 [doi]
LID - 45
AB  - Porcine reproductive and respiratory syndrome (PRRS) is caused by PRRS virus 
      (PRRSV), which infects primarily the respiratory tract of pigs. Thus intranasal 
      (IN) delivery of a potent vaccine-adjuvant formulation is promising. In this 
      study, PRRS-MLV (VR2332) was coadministered +/- an adjuvant Mycobacterium vaccae 
      whole cell lysate or CpG ODN through intramuscular (IM) or IN route as a mist, 
      and challenged with a heterologous PRRSV 1-4-4 IN at 42 days post-vaccination 
      (dpv). At 14 and 26 dpv, vaccine viral RNA copies were one log greater in the 
      plasma of PRRS-MLV IM compared to IN vaccinated pigs, and the infectious 
      replicating vaccine virus was detected only in the IM group. In PRRS-MLV +/- 
      adjuvant IM vaccinated pigs, reduced viral RNA load and absence of the 
      replicating challenged virus was observed at 7, 10 and 14 days post-challenge 
      (dpc). At 14 dpc, in BAL fluid >/= 5 log viral RNA copies were detected in all the 
      pig groups, but the replicating challenged virus was undetectable only in IM 
      groups. Immunologically, virus neutralizing antibody titers in the plasma of IM 
      (but not IN) vaccine groups was >/= 8 against the vaccine and challenged viruses. 
      At 26 dpv, PRRS-MLV IM (without adjuvant) received pigs had significantly 
      increased population of CD4 and CD8 T cells in PBMC. At 14 dpc, relatively 
      increased population of IFN-gamma(+) total lymphocytes, NK, CD4, CD8 and gammadelta T cells 
      were observed in the MLV-IM group. In conclusion, PRRS-MLV IM vaccination induced 
      the virus specific T cell response in pigs, but still it is required to improve 
      its cross-protective efficacy.
FAU - Ouyang, Kang
AU  - Ouyang K
AD  - Food Animal Health Research Program (FAHRP), OARDC, Department of Veterinary 
      Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.
AD  - College of Animal Science and Technology, Guangxi University, Nanning, China.
FAU - Hiremath, Jagadish
AU  - Hiremath J
AD  - Food Animal Health Research Program (FAHRP), OARDC, Department of Veterinary 
      Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.
FAU - Binjawadagi, Basavaraj
AU  - Binjawadagi B
AD  - Food Animal Health Research Program (FAHRP), OARDC, Department of Veterinary 
      Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.
FAU - Shyu, Duan-Liang
AU  - Shyu DL
AD  - Food Animal Health Research Program (FAHRP), OARDC, Department of Veterinary 
      Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.
FAU - Dhakal, Santosh
AU  - Dhakal S
AD  - Food Animal Health Research Program (FAHRP), OARDC, Department of Veterinary 
      Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.
FAU - Arcos, Jesus
AU  - Arcos J
AD  - Department of Microbial Infection and Immunity, The Ohio State University, 
      Columbus, OH, USA.
FAU - Schleappi, Rose
AU  - Schleappi R
AD  - Food Animal Health Research Program (FAHRP), OARDC, Department of Veterinary 
      Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA.
FAU - Holman, Lynette
AU  - Holman L
AD  - Kalmbach Swine Management, L.L.C., Upper Sandusky, OH, 43351, USA.
FAU - Roof, Michael
AU  - Roof M
AD  - Boehringer Ingelheim Vetmedica, Inc., Ames, IA, USA.
FAU - Torrelles, Jordi B
AU  - Torrelles JB
AD  - Department of Microbial Infection and Immunity, The Ohio State University, 
      Columbus, OH, USA.
FAU - Renukaradhya, Gourapura J
AU  - Renukaradhya GJ
AD  - Food Animal Health Research Program (FAHRP), OARDC, Department of Veterinary 
      Preventive Medicine, The Ohio State University, Wooster, OH, 44691, USA. 
      gourapura.1@osu.edu.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160317
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Adjuvants, Immunologic/administration & dosage/*therapeutic use
MH  - Administration, Intranasal/*veterinary
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Cross Protection
MH  - Immunity, Heterologous
MH  - Injections, Intramuscular/*veterinary
MH  - Mycobacterium/*immunology
MH  - Oligodeoxyribonucleotides/administration & dosage/therapeutic use
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Vaccines, Attenuated/administration & dosage/immunology/therapeutic use
MH  - Viral Vaccines/administration & dosage/*immunology/therapeutic use
PMC - PMC4797253
EDAT- 2016/03/19 06:00
MHDA- 2016/10/13 06:00
PMCR- 2016/03/17
CRDT- 2016/03/19 06:00
PHST- 2015/10/22 00:00 [received]
PHST- 2016/01/29 00:00 [accepted]
PHST- 2016/03/19 06:00 [entrez]
PHST- 2016/03/19 06:00 [pubmed]
PHST- 2016/10/13 06:00 [medline]
PHST- 2016/03/17 00:00 [pmc-release]
AID - 10.1186/s13567-016-0331-3 [pii]
AID - 331 [pii]
AID - 10.1186/s13567-016-0331-3 [doi]
PST - epublish
SO  - Vet Res. 2016 Mar 17;47:45. doi: 10.1186/s13567-016-0331-3.