PMID- 26990065
OWN - NLM
STAT- MEDLINE
DCOM- 20170605
LR  - 20181113
IS  - 1945-7170 (Electronic)
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 157
IP  - 5
DP  - 2016 May
TI  - Planar Cell Polarity (PCP) Protein Vangl2 Regulates Ectoplasmic Specialization 
      Dynamics via Its Effects on Actin Microfilaments in the Testes of Male Rats.
PG  - 2140-59
LID - 10.1210/en.2015-1987 [doi]
AB  - Planar cell polarity (PCP) proteins confer polarization of a field of cells (eg, 
      elongating/elongated spermatids) within the plane of an epithelium such as the 
      seminiferous epithelium of the tubule during spermatogenesis. In adult rat 
      testes, Sertoli and germ cells were found to express PCP core proteins (eg, Van 
      Gogh-like 2 [Vangl2]), effectors, ligands, and signaling proteins. Vangl2 
      expressed predominantly by Sertoli cells was localized at the testis-specific, 
      actin-rich ectoplasmic specialization (ES) at the Sertoli-spermatid interface in 
      the adluminal compartment and also Sertoli-Sertoli interface at the blood-testis 
      barrier (BTB) and structurally interacted with actin, N-cadherin, and another 
      PCP/polarity protein Scribble. Vangl2 knockdown (KD) by RNA interference in 
      Sertoli cells cultured in vitro with an established tight junction-permeability 
      barrier led to BTB tightening, whereas its overexpression using a full-length 
      cDNA construct perturbed the barrier function. These changes were mediated 
      through an alteration on the organization actin microfilaments at the ES in 
      Sertoli cells, involving actin-regulatory proteins, epidermal growth factor 
      receptor pathway substrate 8, actin-related protein 3, and Scribble, which in 
      turn affected the function of adhesion protein complexes at the ES during the 
      epithelial cycle of spermatogenesis. Using Polyplus in vivo-jetPEI reagent as a 
      transfection medium to silence Vangl2 in the testis in vivo by RNA interference 
      with high efficacy, Vangl2 KD led to changes in F-actin organization at the ES in 
      the epithelium, impeding spermatid and phagosome transport and spermatid 
      polarity, meiosis, and BTB dynamics. For instance, step 19 spermatids remained 
      embedded in the epithelium alongside with step 9 and 10 spermatids in stages IX-X 
      tubules. In summary, the PCP protein Vangl2 is an ES regulator through its 
      effects on actin microfilaments in the testis.
FAU - Chen, Haiqi
AU  - Chen H
AD  - The Mary M. Wohlford Laboratory for Male Contraceptive Research (H.C., D.D.M., 
      C.Y.C.), Center for Biomedical Research, Population Council, New York, New York 
      10065; and School of Biological Sciences (W.M.L.), University of Hong Kong, 
      Pokfulam, Hong Kong, China.
FAU - Mruk, Dolores D
AU  - Mruk DD
AD  - The Mary M. Wohlford Laboratory for Male Contraceptive Research (H.C., D.D.M., 
      C.Y.C.), Center for Biomedical Research, Population Council, New York, New York 
      10065; and School of Biological Sciences (W.M.L.), University of Hong Kong, 
      Pokfulam, Hong Kong, China.
FAU - Lee, Will M
AU  - Lee WM
AD  - The Mary M. Wohlford Laboratory for Male Contraceptive Research (H.C., D.D.M., 
      C.Y.C.), Center for Biomedical Research, Population Council, New York, New York 
      10065; and School of Biological Sciences (W.M.L.), University of Hong Kong, 
      Pokfulam, Hong Kong, China.
FAU - Cheng, C Yan
AU  - Cheng CY
AD  - The Mary M. Wohlford Laboratory for Male Contraceptive Research (H.C., D.D.M., 
      C.Y.C.), Center for Biomedical Research, Population Council, New York, New York 
      10065; and School of Biological Sciences (W.M.L.), University of Hong Kong, 
      Pokfulam, Hong Kong, China.
LA  - eng
GR  - R01 HD056034/HD/NICHD NIH HHS/United States
GR  - U54 HD029990/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160318
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Actin-Related Protein 3)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (Vangl2 protein, rat)
RN  - 0 (scribble protein, rat)
SB  - IM
MH  - Actin Cytoskeleton/*metabolism
MH  - Actin-Related Protein 3/metabolism
MH  - Animals
MH  - Blood-Testis Barrier/*metabolism
MH  - Cell Polarity/physiology
MH  - Cells, Cultured
MH  - Male
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - RNA Interference
MH  - Rats
MH  - Sertoli Cells/cytology/*metabolism
MH  - Spermatids/cytology/metabolism
MH  - Spermatogenesis/physiology
MH  - Testis/cytology/*metabolism
MH  - Tumor Suppressor Proteins/metabolism
PMC - PMC4870864
EDAT- 2016/03/19 06:00
MHDA- 2017/06/06 06:00
PMCR- 2017/05/01
CRDT- 2016/03/19 06:00
PHST- 2016/03/19 06:00 [entrez]
PHST- 2016/03/19 06:00 [pubmed]
PHST- 2017/06/06 06:00 [medline]
PHST- 2017/05/01 00:00 [pmc-release]
AID - EN-15-1987 [pii]
AID - 10.1210/en.2015-1987 [doi]
PST - ppublish
SO  - Endocrinology. 2016 May;157(5):2140-59. doi: 10.1210/en.2015-1987. Epub 2016 Mar 
      18.