PMID- 26990081
OWN - NLM
STAT- MEDLINE
DCOM- 20161215
LR  - 20210109
IS  - 1099-0844 (Electronic)
IS  - 0263-6484 (Print)
IS  - 0263-6484 (Linking)
VI  - 34
IP  - 3
DP  - 2016 Apr
TI  - Synergistic effect of bioactive lipid and condition medium on cardiac 
      differentiation of human mesenchymal stem cells from different tissues.
PG  - 163-72
LID - 10.1002/cbf.3175 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUCMSCs) and human adipose tissue 
      mesenchymal stem cells (hATMSCs) have the potential to differentiate into 
      cardiomyocytes, making them promising therapeutic candidates for treating damaged 
      cardiac tissues. Currently, however, the differentiated cells induced from 
      hUCMSCs or hATMSCs can hardly display functional characteristics similar to 
      cardiomyocytes. In this study, we have investigated the effects of bioactive 
      lipid sphingosine-1-phosphate (S1P) on cardiac differentiations of hUCMSCs and 
      hATMSCs in condition medium composed of cardiac myocytes culture medium or 
      5-azacytidine. Cardiac differentiations were identified through 
      immunofluorescence staining, and the results were observed with fluorescence 
      microscopy and confocal microscopy. Synergistic effects of S1P and condition 
      medium on cell viability were evaluated by MTT assays. Functional characteristics 
      similar to cardiomyocytes were evaluated through detecting calcium transient. The 
      differentiated hUCMSCs or hATMSCs in each group into cardiomyocytes showed 
      positive expressions of cardiac specific proteins, including alpha-actin, connexin-43 
      and myosin heavy chain-6 (MYH-6). MTT assays showed that suitable differentiation 
      time was 14 days and that the optimal concentration of S1P was 0.5 muM. Moreover, 
      incorporation of S1P and cardiac myocytes culture medium gave rise to calcium 
      transients, an important marker for displaying in vivo electrophysiological 
      properties. This feature was not observed in the S1P-5-azacytidine group, 
      indicating the possible lack of cellular stimuli such as transforming growth 
      factor-beta, TGF-beta.
CI  - Copyright (c) 2016 John Wiley & Sons, Ltd.
FAU - Jiang, Lili
AU  - Jiang L
AD  - Dalian R&D Center for Stem Cell and Tissue Engineering, Faculty of Chemical 
      Environmental and Biological Science and Technology, Dalian University of 
      Technology, Dalian, China.
FAU - Wang, Yanwen
AU  - Wang Y
AD  - Cardiovascular and Genetic Medicine Research Groups, School of Biomedicine, 
      University of Manchester, Manchester, UK.
FAU - Pan, Fang
AU  - Pan F
AD  - Biological Physics Group, School of Physics and Astronomy, University of 
      Manchester, Manchester, UK.
FAU - Zhao, Xiubo
AU  - Zhao X
AD  - Department of Chemical & Biological Engineering, University of Sheffield, Mappin 
      Street, Sheffield, S1 3JD, UK.
FAU - Zhang, Henggui
AU  - Zhang H
AD  - Biological Physics Group, School of Physics and Astronomy, University of 
      Manchester, Manchester, UK.
FAU - Lei, Ming
AU  - Lei M
AD  - Cardiovascular and Genetic Medicine Research Groups, School of Biomedicine, 
      University of Manchester, Manchester, UK.
FAU - Liu, Tianqing
AU  - Liu T
AD  - Dalian R&D Center for Stem Cell and Tissue Engineering, Faculty of Chemical 
      Environmental and Biological Science and Technology, Dalian University of 
      Technology, Dalian, China.
FAU - Lu, Jian R
AU  - Lu JR
AD  - Biological Physics Group, School of Physics and Astronomy, University of 
      Manchester, Manchester, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160316
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Lipids)
SB  - IM
MH  - Adipose Tissue/*cytology/drug effects
MH  - Cell Differentiation/*drug effects
MH  - Cell Survival/drug effects
MH  - Culture Media, Conditioned/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Lipids/*pharmacology
MH  - Mesenchymal Stem Cells/*cytology/*drug effects
MH  - Myocytes, Cardiac/*cytology/drug effects
MH  - Structure-Activity Relationship
PMC - PMC5031220
OTO - NOTNLM
OT  - 5-azacytidine
OT  - bioactive lipid
OT  - calcium transients
OT  - cardiac differentiation
OT  - synergistic effect
EDAT- 2016/03/19 06:00
MHDA- 2016/12/16 06:00
PMCR- 2016/09/21
CRDT- 2016/03/19 06:00
PHST- 2015/11/13 00:00 [received]
PHST- 2016/01/22 00:00 [revised]
PHST- 2016/02/08 00:00 [accepted]
PHST- 2016/03/19 06:00 [entrez]
PHST- 2016/03/19 06:00 [pubmed]
PHST- 2016/12/16 06:00 [medline]
PHST- 2016/09/21 00:00 [pmc-release]
AID - CBF3175 [pii]
AID - 10.1002/cbf.3175 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2016 Apr;34(3):163-72. doi: 10.1002/cbf.3175. Epub 2016 Mar 
      16.