PMID- 26990435
OWN - NLM
STAT- MEDLINE
DCOM- 20160728
LR  - 20231111
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 3
DP  - 2016
TI  - Photodynamic Effect of Ni Nanotubes on an HeLa Cell Line.
PG  - e0150295
LID - 10.1371/journal.pone.0150295 [doi]
LID - e0150295
AB  - Nickel nanomaterials are promising in the biomedical field, especially in cancer 
      diagnostics and targeted therapy, due to their distinctive chemical and physical 
      properties. In this experiment, the toxicity of nickel nanotubes (Ni NTs) were 
      tested in an in vitro cervical cancer model (HeLa cell line) to optimize the 
      parameters of photodynamic therapy (PDT) for their greatest effectiveness. Ni NTs 
      were synthesized by electrodeposition. Morphological analysis and magnetic 
      behavior were examined using a Scanning electron microscope (SEM), an energy 
      dispersive X-ray analysis (EDAX) and a vibrating sample magnetometer (VSM) 
      analysis. Phototoxic and cytotoxic effects of nanomaterials were studied using 
      the Ni NTs alone as well as in conjugation with aminolevulinic acid (5-ALA); this 
      was performed both in the dark and under laser exposure. Toxic effects on the 
      HeLa cell model were evaluated by a neutral red assay (NRA) and by detection of 
      intracellular reactive oxygen species (ROS) production. Furthermore, 10-200 nM of 
      Ni NTs was prepared in solution form and applied to HeLa cells in 96-well plates. 
      Maximum toxicity of Ni NTs complexed with 5-ALA was observed at 100 J/cm2 and 200 
      nM. Up to 65-68% loss in cell viability was observed. Statistical analysis was 
      performed on the experimental results to confirm the worth and clarity of 
      results, with p-values = 0.003 and 0.000, respectively. Current results pave the 
      way for a more rational strategy to overcome the problem of drug bioavailability 
      in nanoparticulate targeted cancer therapy, which plays a dynamic role in 
      clinical practice.
FAU - Hammad Aziz, Muhammad
AU  - Hammad Aziz M
AD  - Department of Physics, College of Science in Zulfi, Majmaah University, Zulfi, 
      Saudi Arabia.
AD  - Department of Physics, COMSATS Institute of Information and Technology, Lahore, 
      Pakistan.
FAU - Fakhar-E-Alam, M
AU  - Fakhar-E-Alam M
AD  - Institute of Fundamental and Frontier Science, University of Electronic Science 
      and Technology of China, 610054 Chengdu, China.
AD  - Department of Physics, GC University Faisalabad, Pakistan.
FAU - Fatima, Mahvish
AU  - Fatima M
AD  - Department of Physics, The Islamia University of Bahawalpur, Bahawalpur, 
      Pakistan.
FAU - Shaheen, Fozia
AU  - Shaheen F
AD  - Department of Physics, GC University Lahore, Lahore, Pakistan.
FAU - Iqbal, Seemab
AU  - Iqbal S
AD  - Department of Physics, GC University Faisalabad, Pakistan.
FAU - Atif, M
AU  - Atif M
AD  - Department of Physics and Astronomy, College of Science, King Saud University, 
      Riyadh, Saudi Arabia.
AD  - National Institute of Laser and Optronics, Nilore, Islamabad, Pakistan.
FAU - Talha, Muhammad
AU  - Talha M
AD  - Deanship of Scientific Research, King Saud University, Riyadh 11421, Saudi 
      Arabia.
FAU - Mansoor Ali, Syed
AU  - Mansoor Ali S
AD  - Department of Physics and Astronomy, College of Science, King Saud University, 
      Riyadh, Saudi Arabia.
FAU - Afzal, Muhammad
AU  - Afzal M
AD  - Department of Physics, The Islamia University of Bahawalpur, Bahawalpur, 
      Pakistan.
FAU - Majid, Abdul
AU  - Majid A
AD  - Department of Physics, College of Science in Zulfi, Majmaah University, Zulfi, 
      Saudi Arabia.
FAU - Shelih Al Harbi, Thamir
AU  - Shelih Al Harbi T
AD  - Department of Physics, College of Science in Zulfi, Majmaah University, Zulfi, 
      Saudi Arabia.
FAU - Ismail, Muhammad
AU  - Ismail M
AD  - Institute of Biomedical and Genetic Engineering (IBGE), 24 Mauve Area, G-9/1, 
      Islamabad, Pakistan.
FAU - Wang, Zhiming M
AU  - Wang ZM
AD  - Institute of Fundamental and Frontier Science, University of Electronic Science 
      and Technology of China, 610054 Chengdu, China.
FAU - AlSalhi, M S
AU  - AlSalhi MS
AD  - Department of Physics and Astronomy, College of Science, King Saud University, 
      Riyadh, Saudi Arabia.
FAU - Alahmed, Z A
AU  - Alahmed ZA
AD  - Department of Physics and Astronomy, College of Science, King Saud University, 
      Riyadh, Saudi Arabia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160318
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Photosensitizing Agents)
RN  - 7OV03QG267 (Nickel)
SB  - IM
MH  - Female
MH  - HeLa Cells
MH  - Humans
MH  - *Nanotubes
MH  - Nickel/*pharmacology
MH  - *Photochemotherapy
MH  - Photosensitizing Agents/therapeutic use
MH  - Uterine Cervical Neoplasms/*drug therapy
PMC - PMC4798439
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/03/19 06:00
MHDA- 2016/07/29 06:00
PMCR- 2016/03/18
CRDT- 2016/03/19 06:00
PHST- 2015/11/25 00:00 [received]
PHST- 2016/02/11 00:00 [accepted]
PHST- 2016/03/19 06:00 [entrez]
PHST- 2016/03/19 06:00 [pubmed]
PHST- 2016/07/29 06:00 [medline]
PHST- 2016/03/18 00:00 [pmc-release]
AID - PONE-D-15-51366 [pii]
AID - 10.1371/journal.pone.0150295 [doi]
PST - epublish
SO  - PLoS One. 2016 Mar 18;11(3):e0150295. doi: 10.1371/journal.pone.0150295. 
      eCollection 2016.