PMID- 26990735
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20191210
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1017-1018
DP  - 2016 Apr 1
TI  - UFLC-MS/MS analysis of four tanshinone components in Salvia miltiorrhizae after 
      ultrasound-assisted extraction.
PG  - 204-210
LID - S1570-0232(16)30148-9 [pii]
LID - 10.1016/j.jchromb.2016.03.008 [doi]
AB  - A sensitive and rapid ultra-fast liquid chromatography with tandem mass 
      spectrometry (UFLC-MS/MS) method was developed for simultaneous qualitative and 
      quantitative of four characteristic tanshione components including tanshinone 
      IIA, cryptotanshinone, tanshinone I and dihydrotanshinone I in Salvia 
      miltiorrhizae after ultrasound-assisted extraction. By using a C18 column, the 
      four analytes were separated by gradient elution with acetonitrile and water both 
      containing 0.1% formic acid at the flow rate of 0.3mL/min. Multiple-reaction 
      monitoring (MRM) was used for quantification, and an information-dependent 
      acquisition (IDA) method was used to trigger enhanced product ion scans (EPI) for 
      supplementary characteristic identification for qualitative research. Calibration 
      curves showed good linearities with correlation coefficients (r) higher than 
      0.9990. The method showed high sensitivity with limits of detection (LODs) and 
      quantification (LOQs) less than 0.0002ng/mL and 0.0008ng/mL, respectively, as 
      well as good precision and reproducibility. Mean recoveries for four analytes 
      ranged from 92.5% to 106.2% with relative standard deviations (RSDs) lower than 
      14.59%. Real application of the developed method in 32 batches of S. 
      miltiorrhizae samples demonstrated that the total contents of four analytes in 
      all samples were in the range of 2.258-52.342mg/g. Ultrasound-assisted extraction 
      technique took a small amount of sample and low time but giving high extraction 
      efficiency. Combining with UFLC-MS/MS method in MRM-IDA-EPI mode, more components 
      in other complicated matrices can be simultaneously analyzed for qualitation and 
      quantitation in one run.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Zhang, Shanshan
AU  - Zhang S
AD  - Pharmacy College, Liaoning Medical University, Jinzhou 121001, China; Institute 
      of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Beijing, 100193 China.
FAU - Liu, Qiutao
AU  - Liu Q
AD  - Pharmacy College, Liaoning Medical University, Jinzhou 121001, China; Institute 
      of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking 
      Union Medical College, Beijing, 100193 China.
FAU - Luo, Hongli
AU  - Luo H
AD  - Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 
      611137, China.
FAU - Chen, Ping
AU  - Chen P
AD  - Guizhou Xinbang Pharmaceutical Co. Ltd., Guiyang 550014,China.
FAU - Wu, Xiaoru
AU  - Wu X
AD  - Guizhou Xinbang Pharmaceutical Co. Ltd., Guiyang 550014,China.
FAU - Yang, Meihua
AU  - Yang M
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & 
      Peking Union Medical College, Beijing, 100193 China.
FAU - Kong, Weijun
AU  - Kong W
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & 
      Peking Union Medical College, Beijing, 100193 China. Electronic address: 
      kongwj302@126.com.
FAU - Guo, Weiying
AU  - Guo W
AD  - Pharmacy College, Liaoning Medical University, Jinzhou 121001, China. Electronic 
      address: wyinguo@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20160309
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Abietanes)
RN  - 03UUH3J385 (tanshinone)
SB  - IM
MH  - Abietanes/*analysis
MH  - Calibration
MH  - Limit of Detection
MH  - Reproducibility of Results
MH  - Salvia miltiorrhiza/*chemistry
MH  - Tandem Mass Spectrometry/*methods
MH  - *Ultrasonics
OTO - NOTNLM
OT  - MRM-IDA-EPI
OT  - Salvia miltiorrhizae
OT  - Tanshinone
OT  - UFLC-MS/MS
OT  - Ultrasound-assisted extraction
EDAT- 2016/03/19 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/03/19 06:00
PHST- 2015/12/14 00:00 [received]
PHST- 2016/03/07 00:00 [revised]
PHST- 2016/03/07 00:00 [accepted]
PHST- 2016/03/19 06:00 [entrez]
PHST- 2016/03/19 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S1570-0232(16)30148-9 [pii]
AID - 10.1016/j.jchromb.2016.03.008 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Apr 1;1017-1018:204-210. doi: 
      10.1016/j.jchromb.2016.03.008. Epub 2016 Mar 9.