PMID- 26990933
OWN - NLM
STAT- MEDLINE
DCOM- 20170118
LR  - 20191210
IS  - 1097-0339 (Electronic)
IS  - 1097-0339 (Linking)
VI  - 44
IP  - 6
DP  - 2016 Jun
TI  - Reliability of immunocytochemistry and fluorescence in situ hybridization on 
      fine-needle aspiration cytology samples of breast cancers: A comparative study.
PG  - 466-71
LID - 10.1002/dc.23463 [doi]
AB  - INTRODUCTION: To characterize breast tumors and metastases, fine-needle 
      aspiration cytology (FNAB) can be a favorable first choice. However, the 
      diagnostic accuracy of ancillary tests applied to FNAB samples is yet to be 
      validated. PATIENTS AND METHODS: We examined 110 breast cancer patients' paired 
      cytological and surgical resection specimens evaluated between 2005 and 2014. 
      Comparison of ER and Her2 immunocytochemical (ICC) and immunohistochemical (IHC) 
      staining and HER2 fluorescence in situ hybridization (FISH) was performed. 
      RESULTS: Significant difference (p < 0.001) and moderate correlation (kappa = 0.446) 
      were noted between results of 97 paired ICC an IHC reactions for ER expression. 
      ICC for ER status had a sensitivity of 75%, specificity of 100%, positive 
      predictive value (PPV) of 100%, and negative predictive value (NPV) of 38.2%. 
      Significant difference (p = 0.012) and moderate correlation (kappa = 0.541) were 
      found between results of 77 paired ICC an IHC reactions for Her2 expression. The 
      Her2 ICC had a sensitivity of 54%, specificity of 95.4%, PPV of 66.7%, and NPV of 
      92.6%. The results of FISH carried out on 23 paired samples of FNAB and surgical 
      specimens indicated perfect correlation (kappa = 1.000) and no significant difference 
      (p = 1.000). FISH performed on FNAB has sensitivity, specificity, PPV, and NPV of 
      100%. CONCLUSION: The correlation of ICC and IHC is moderate regarding ER and 
      Her2 expression of the same tumor. FISH performed on FNAB samples is suitable to 
      categorize primary and metastatic breast cancer in regard of HER2 gene 
      amplification status and can be used as a predictive test in respect of therapies 
      targeting HER2. Diagn. Cytopathol. 2016;44:466-471. (c) 2016 Wiley Periodicals, 
      Inc.
CI  - (c) 2016 Wiley Periodicals, Inc.
FAU - Acs, Balazs
AU  - Acs B
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
FAU - Szekely, Nora
AU  - Szekely N
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
FAU - Szasz, Attila Marcell
AU  - Szasz AM
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
FAU - Lotz, Gabor
AU  - Lotz G
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
FAU - Szekely, Tamas
AU  - Szekely T
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
FAU - Istok, Roland
AU  - Istok R
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
FAU - Szekely, Eszter
AU  - Szekely E
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
FAU - Madaras, Lilla
AU  - Madaras L
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
FAU - Kulka, Janina
AU  - Kulka J
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
FAU - Jaray, Balazs
AU  - Jaray B
AD  - 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20160316
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Receptors, Estrogen)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Biopsy, Fine-Needle
MH  - Breast Neoplasms/metabolism/*pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Receptor, ErbB-2/genetics/metabolism
MH  - Receptors, Estrogen/genetics/metabolism
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - breast cancer
OT  - cytology
OT  - fine-needle aspiration biopsy
OT  - fluorescence in situ hybridization
OT  - immunocytochemistry
OT  - immunohistochemistry
OT  - surgical resection specimen
EDAT- 2016/03/19 06:00
MHDA- 2017/01/19 06:00
CRDT- 2016/03/19 06:00
PHST- 2015/04/14 00:00 [received]
PHST- 2016/01/15 00:00 [revised]
PHST- 2016/02/12 00:00 [accepted]
PHST- 2016/03/19 06:00 [entrez]
PHST- 2016/03/19 06:00 [pubmed]
PHST- 2017/01/19 06:00 [medline]
AID - 10.1002/dc.23463 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2016 Jun;44(6):466-71. doi: 10.1002/dc.23463. Epub 2016 Mar 16.