PMID- 26991265
OWN - NLM
STAT- MEDLINE
DCOM- 20180208
LR  - 20180226
IS  - 1399-3089 (Electronic)
IS  - 0908-665X (Linking)
VI  - 23
IP  - 2
DP  - 2016 Mar
TI  - Microbiological characterization of a newly established pig breed, Aachen 
      Minipigs.
PG  - 159-67
LID - 10.1111/xen.12233 [doi]
AB  - BACKGROUND: To alleviate the shortage of human donor organs or tissues for the 
      treatment of organ and tissue failure including diabetes, pigs are considered 
      suitable donor animals. As organs from conventional pigs are usually too large, 
      those from minipigs may be better suited. We recently characterized the Gottingen 
      Minipigs, a breed well characterized concerning the presence of zoonotic 
      microorganisms and found hepatitis E virus (HEV) and porcine cytomegalovirus 
      (PCMV) in some animals. Here, we characterize another minipig, the Aachen Minipig 
      (AaMP), a pig breed recently established close to the town Aachen in Germany. 
      METHODS: The animals were tested for the prevalence and expression of porcine 
      endogenous retroviruses (PERVs) and the presence of some selected microorganisms, 
      among them HEV, PCMV, and porcine lymphotropic herpesviruses (PLHVs) using highly 
      sensitive and specific PCR and RT-PCR methods. In addition, we screened for 
      antibodies against HEV and PLHV. RESULTS: PERV-A, PERV-B, and PERV-C sequences 
      were found in the genome of all Aachen Minipigs. HEV RNA was found by real-time 
      RT-PCR in most, and DNA of PCMV, PLHV-2, and PLHV-3 was found by PCR in some 
      animals. The animals were free of eight other microorganisms tested, but some 
      were seropositive for porcine circovirus 2 (PCV2), porcine reproductive and 
      respiratory syndrome virus (PRRSV), and porcine epidemic diarrhea virus (PEDV). 
      CONCLUSION: Based on medical examinations by veterinarians, the AaMP are in a 
      good health status and seem to harbor only few microorganisms. To improve their 
      status for use as donor pigs in xenotransplantation, the viruses detected might 
      be eliminated by selection of negative animals, Cesarean section, and 
      vaccination.
CI  - (c) 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Plotzki, Elena
AU  - Plotzki E
AD  - Robert Koch Institute, HIV and other Retroviruses, Berlin, Germany.
FAU - Heinrichs, Gerd
AU  - Heinrichs G
AD  - Aachen Minipig, Heinsberg, Germany.
FAU - Kubickova, Barbara
AU  - Kubickova B
AD  - Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 
      Institute for Novel and Emerging Infectious Diseases, Greifswald - Insel Riems, 
      Germany.
FAU - Ulrich, Rainer G
AU  - Ulrich RG
AD  - Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 
      Institute for Novel and Emerging Infectious Diseases, Greifswald - Insel Riems, 
      Germany.
FAU - Denner, Joachim
AU  - Denner J
AD  - Robert Koch Institute, HIV and other Retroviruses, Berlin, Germany.
LA  - eng
SI  - GENBANK/AJ293656
SI  - GENBANK/AJ293657
SI  - GENBANK/AM229312
SI  - GENBANK/AY008846
SI  - GENBANK/AY170316.1
SI  - GENBANK/AY170317.1
SI  - GENBANK/AY570980.1
SI  - GENBANK/KF017583.1
SI  - GENBANK/KT633715.1
SI  - GENBANK/NM_001206359.1
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160317
PL  - Denmark
TA  - Xenotransplantation
JT  - Xenotransplantation
JID - 9438793
SB  - IM
MH  - Animals
MH  - Endogenous Retroviruses
MH  - Female
MH  - Hepatitis E virus
MH  - Herpesviridae
MH  - Porcine epidemic diarrhea virus
MH  - Pregnancy
MH  - Swine
MH  - Swine Diseases/*virology
MH  - Swine, Miniature
OTO - NOTNLM
OT  - PERV
OT  - cytomegalovirus
OT  - hepatitis E virus
OT  - lymphotropic herpesviruses
OT  - porcine viruses
OT  - virus safety
OT  - xenotransplantation
EDAT- 2016/03/19 06:00
MHDA- 2018/02/09 06:00
CRDT- 2016/03/19 06:00
PHST- 2015/07/14 00:00 [received]
PHST- 2016/02/15 00:00 [accepted]
PHST- 2016/03/19 06:00 [entrez]
PHST- 2016/03/19 06:00 [pubmed]
PHST- 2018/02/09 06:00 [medline]
AID - 10.1111/xen.12233 [doi]
PST - ppublish
SO  - Xenotransplantation. 2016 Mar;23(2):159-67. doi: 10.1111/xen.12233. Epub 2016 Mar 
      17.