PMID- 26994470
OWN - NLM
STAT- MEDLINE
DCOM- 20170404
LR  - 20170817
IS  - 1879-2472 (Electronic)
IS  - 0049-3848 (Linking)
VI  - 141
DP  - 2016 May
TI  - Meal-induced platelet activation in diabetes mellitus type 1 or type 2 is related 
      to postprandial insulin rather than glucose levels.
PG  - 93-7
LID - S0049-3848(16)30069-X [pii]
LID - 10.1016/j.thromres.2016.03.009 [doi]
AB  - AIM: Postprandial platelet activation was related to postprandial insulin rather 
      than glucose levels in a previous meal insulin study in type 2 diabetes mellitus 
      (T2DM). We therefore compared postprandial platelet activation in type 1 (T1DM) 
      patients without insulin secretion and T2DM patients with high postprandial 
      insulin levels. MATERIAL AND METHODS: Patients with T1DM (n=11) and T2DM (n=12) 
      were studied before and 90min after a standardized meal without premeal insulin. 
      Five T1DM patients volunteered for a restudy with their regular premeal insulin. 
      Platelet activation was assessed by flow cytometry, with and without the 
      thromboxane analogue U46619 or ADP, and by whole blood aggregometry 
      (Multiplate(R)). Effects of insulin (100muU/mL) in vitro were also studied. RESULTS: 
      Before the meal, glucose, insulin and platelet activation markers other than 
      platelet-leukocyte aggregates (PLAs) were similar in T1DM and T2DM; PLAs were 
      higher in T1DM. Postprandial glucose levels increased more markedly in T1DM (to 
      22.1+/-1.4 vs. 11.2+/-0.6mmol/L) while insulin levels increased only in T2DM (from 
      24.4+/-4.4 to 68.8+/-12.3muU/mL). Platelet P-selectin expression, fibrinogen binding 
      and PLA formation stimulated by U46619 were markedly enhanced (approximately 
      doubled) and whole blood aggregation stimulated by U46619 was increased (p<0.05 
      for all) after the meal in T2DM patients but not in T1DM patients. The pilot 
      study with premeal insulin in T1DM patients showed postprandial platelet 
      activation when postprandial insulin levels increased. In vitro insulin mildly 
      activated platelets in both groups. CONCLUSION: Postprandial platelet activation 
      via the thromboxane pathway is related to postprandial hyperinsulinemia and not 
      to postprandial hyperglycaemia in patients with diabetes.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Spectre, Galia
AU  - Spectre G
AD  - Department of Medicine Solna, Clinical Pharmacology Unit, Karolinska University 
      Hospital, Solna, Stockholm, Sweden; Department of Hematology, Coagulation Unit, 
      Hadassah-Hebrew University Medical Center, Jerusalem, Israel; Hematology 
      Institute, Thrombosis and Heamostasis Unit, Rabin Medical Center, Beilinsn 
      Hospital, Petach Tikva, Israel.
FAU - Stalesen, Ragnhild
AU  - Stalesen R
AD  - Department of Medicine Solna, Clinical Pharmacology Unit, Karolinska University 
      Hospital, Solna, Stockholm, Sweden.
FAU - Ostenson, Claes-Goran
AU  - Ostenson CG
AD  - Department of Molecular Medicine and Surgery, Endocrinology and Diabetology Unit, 
      Karolinska Institutet, Karolinska University Hospital, Solna, Stockholm, Sweden.
FAU - Hjemdahl, Paul
AU  - Hjemdahl P
AD  - Department of Medicine Solna, Clinical Pharmacology Unit, Karolinska University 
      Hospital, Solna, Stockholm, Sweden. Electronic address: Paul.Hjemdahl@ki.se.
LA  - eng
PT  - Journal Article
DEP - 20160308
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Blood Glucose/analysis
MH  - Blood Platelets/pathology
MH  - Diabetes Mellitus, Type 1/*blood/physiopathology
MH  - Diabetes Mellitus, Type 2/*blood/physiopathology
MH  - Eating
MH  - Female
MH  - Humans
MH  - Insulin/*blood
MH  - Male
MH  - Middle Aged
MH  - Pilot Projects
MH  - *Platelet Activation
MH  - *Postprandial Period
OTO - NOTNLM
OT  - Glucose
OT  - Insulin
OT  - Platelet activation
OT  - Postprandial
EDAT- 2016/03/20 06:00
MHDA- 2017/04/05 06:00
CRDT- 2016/03/20 06:00
PHST- 2015/11/17 00:00 [received]
PHST- 2016/02/14 00:00 [revised]
PHST- 2016/03/07 00:00 [accepted]
PHST- 2016/03/20 06:00 [entrez]
PHST- 2016/03/20 06:00 [pubmed]
PHST- 2017/04/05 06:00 [medline]
AID - S0049-3848(16)30069-X [pii]
AID - 10.1016/j.thromres.2016.03.009 [doi]
PST - ppublish
SO  - Thromb Res. 2016 May;141:93-7. doi: 10.1016/j.thromres.2016.03.009. Epub 2016 Mar 
      8.