PMID- 26994898
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20190816
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 22
DP  - 2016 Mar 20
TI  - Effects of Shock Waves on Expression of IL-6, IL-8, MCP-1, and TNF-alpha Expression 
      by Human Periodontal Ligament Fibroblasts: An In Vitro Study.
PG  - 914-21
AB  - BACKGROUND: Extracorporeal shock wave therapy (ESWT) can modulate cell behavior 
      through mechanical information transduction. Human periodontal ligament 
      fibroblasts (hPDLF) are sensible to mechanical stimulus and can express 
      pro-inflammatory molecules in response. The aim of this study was to evaluate the 
      impacts of shock waves on interleukin-6 (IL-6), interleukin-8 (IL-8), monocyte 
      chemotactic protein 1 (MCP-1), and tumor necrosis factor-alpha (TNF-alpha) expression 
      by hPDLF. MATERIAL/METHODS: After being treated by shock waves with different 
      parameters (100-500 times, 0.05-0.19 mJ/mm(2)), cell viability was tested using 
      CCK-8. IL-6, IL-8, MCP-1, and TNF-alpha gene expression was analyzed by quantitative 
      real-time polymerase chain reaction (qRT-PCR) and IL-6 and IL-8 protein was 
      measured by enzyme-linked immunosorbent assay (ELISA) at different time points. 
      RESULTS: Shock waves with the parameters used in this study had no significant 
      effects on the viability of hPDLF. A statistical inhibition of IL-6, IL-8, MCP-1, 
      and TNF-alpha expression during the first few hours was observed (P<0.05). Expression 
      of IL-8 was significantly elevated in the group receiving the most pulses of 
      shock wave (500 times) after 4 h (P<0.05). At 8 h and 24 h, all treated groups 
      demonstrated significantly enhanced IL-6 expression (P<0.05). TNF-alpha expression in 
      the groups receiving more shock pulses (300, 500 times) or the highest energy 
      shock treatment (0.19 mJ/mm(2)) was statistically decreased (P<0.05) at 24 h. 
      CONCLUSIONS: Under the condition of this study, a shock wave with energy density 
      no higher than 0.19 mJ/mm(2) and pulses no more than 500 times elicited no 
      negative effects on cell viability of hPDLF. After a uniform initial inhibition 
      impact on expression of inflammatory mediators, a shock wave could cause 
      dose-related up-regulation of IL-6 and IL-8 and down-regulation of TNF-alpha.
FAU - Cai, Zhiyu
AU  - Cai Z
AD  - School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, 
      China (mainland).
FAU - Falkensammer, Frank
AU  - Falkensammer F
AD  - Department of Orthodontics, Bernhard Gottlieb University Clinic of Dentistry, 
      Medical University of Vienna, Vienna, Austria.
FAU - Andrukhov, Oleh
AU  - Andrukhov O
AD  - Competence Centre of Periodontal Research, Bernhard Gottlieb School of Dentistry, 
      Medical University of Vienna, Vienna, Austria.
FAU - Chen, Jiang
AU  - Chen J
AD  - School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, 
      China (mainland).
FAU - Mittermayr, Rainer
AU  - Mittermayr R
AD  - The Austrian Cluster for Tissue Regeneration, Ludwig Boltzmann Institute for 
      Experimental and Clinical Traumatology/Austrian Workers' Compensation Board 
      (AUVA) Research Center, Vienna, Austria.
FAU - Rausch-Fan, Xiaohui
AU  - Rausch-Fan X
AD  - Competence Centre of Periodontal Research, Bernhard Gottlieb School of Dentistry, 
      Medical University of Vienna, Vienna, Austria.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160320
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Cell Proliferation
MH  - Cell Survival/genetics
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Fibroblasts/cytology/*metabolism
MH  - *Gene Expression Regulation
MH  - *High-Energy Shock Waves
MH  - Humans
MH  - Interleukin-6/*genetics/metabolism
MH  - Interleukin-8/*genetics/metabolism
MH  - Male
MH  - Periodontal Ligament/*cytology
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*genetics/metabolism
PMC - PMC4805137
EDAT- 2016/03/21 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/03/20
CRDT- 2016/03/21 06:00
PHST- 2016/03/21 06:00 [entrez]
PHST- 2016/03/21 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/03/20 00:00 [pmc-release]
AID - 897507 [pii]
AID - 10.12659/msm.897507 [doi]
PST - epublish
SO  - Med Sci Monit. 2016 Mar 20;22:914-21. doi: 10.12659/msm.897507.