PMID- 26998004
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 11
IP  - 3
DP  - 2016 Mar
TI  - Expression profile analysis based on DNA microarray for patients undergoing 
      off-pump coronary artery bypass surgery.
PG  - 864-872
AB  - Off-pump coronary artery bypass (OPCAB) surgery is the most effective treatment 
      for coronary heart disease. The aim of this study was to explore the effects of 
      OPCAB on the basis of the associated molecular mechanisms. GSE12486 expression 
      profiles downloaded from the Gene Expression Omnibus database (GEO) were analyzed 
      to identify the differentially expressed genes (DEGs). Principal component 
      analysis (PCA) was conducted based on the expression profiles of DEGs. Function 
      and pathway enrichment of upregulated DEGs was performed, followed by 
      protein-protein interaction (PPI) network construction. Gene Set Enrichment 
      Analysis (GSEA) was used for miRNA enrichment analysis based on expression 
      profiles and prediction of their association with the disease. Cytoscape was 
      applied to construct miRNA regulatory networks of DEGs. In total 64 DEGs were 
      identified, including 63 upregulated and 1 downregulated gene. The first 
      principal component in the PCA analysis was able to distinguish between pre- and 
      post-OPCAB samples. Upregulated DEGs mainly enriched 20 Gene Ontology terms, such 
      as chemokine activity, and 5 pathways including the chemokine signaling pathway. 
      The constructed PPI network contained 234 edges and 55 nodes, and 10 upregulated 
      hub nodes, including FBJ murine osteosarcoma viral oncogene homolog (FOS), were 
      screened. A total of 36 miRNAs, including MIR-224 and MIR-7, were screened by 
      GSEA enrichment analysis. A miRNA regulatory network including 176 edges and 97 
      nodes was constructed, showing the regulatory relationships between miRNAs and 
      DEGs. For example, early growth response 2 (EGR2) was regulated by 8 miRNAs 
      including MIR-150, MIR-142-3P, MIR-367 and MIR-224. The identified DEGs might 
      play important roles in patients pre- and post-OPCAB surgery via the regulation 
      of associated genes.
FAU - Sun, Yunpeng
AU  - Sun Y
AD  - Department of Cardiac Surgery, The First Hospital of Jilin University, Changchun, 
      Jilin 130021, P.R. China.
FAU - Gao, Yongsheng
AU  - Gao Y
AD  - Department of Cardiac Surgery, The First Hospital of Jilin University, Changchun, 
      Jilin 130021, P.R. China.
FAU - Sun, Jingnan
AU  - Sun J
AD  - Cancer Center, The First Hospital of Jilin University, Changchun, Jilin 130021, 
      P.R. China.
FAU - Liu, Xuguang
AU  - Liu X
AD  - Department of Cardiac Surgery, The First Hospital of Jilin University, Changchun, 
      Jilin 130021, P.R. China.
FAU - Ma, Dashi
AU  - Ma D
AD  - Department of Cardiac Surgery, The First Hospital of Jilin University, Changchun, 
      Jilin 130021, P.R. China.
FAU - Ma, Chunye
AU  - Ma C
AD  - Department of Cardiac Surgery, The First Hospital of Jilin University, Changchun, 
      Jilin 130021, P.R. China.
FAU - Wang, Yong
AU  - Wang Y
AD  - Department of Cardiac Surgery, The First Hospital of Jilin University, Changchun, 
      Jilin 130021, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160118
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4774345
OTO - NOTNLM
OT  - miRNA regulatory network
OT  - off-pump coronary artery bypass
OT  - pathway analysis
OT  - protein-protein interaction network
EDAT- 2016/03/22 06:00
MHDA- 2016/03/22 06:01
PMCR- 2016/01/18
CRDT- 2016/03/22 06:00
PHST- 2014/10/27 00:00 [received]
PHST- 2015/12/03 00:00 [accepted]
PHST- 2016/03/22 06:00 [entrez]
PHST- 2016/03/22 06:00 [pubmed]
PHST- 2016/03/22 06:01 [medline]
PHST- 2016/01/18 00:00 [pmc-release]
AID - ETM-0-0-3003 [pii]
AID - 10.3892/etm.2016.3003 [doi]
PST - ppublish
SO  - Exp Ther Med. 2016 Mar;11(3):864-872. doi: 10.3892/etm.2016.3003. Epub 2016 Jan 
      18.