PMID- 26998006
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 11
IP  - 3
DP  - 2016 Mar
TI  - CD80 and CD86 knockdown in dendritic cells regulates Th1/Th2 cytokine production 
      in asthmatic mice.
PG  - 878-884
AB  - Dendritic cells (DCs) are associated with the activation and differentiation of T 
      helper (Th) cells. Cluster of differentiation (CD)80 and CD86, the co-stimulatory 
      molecules highly expressed in DCs, have are prominent in promoting the 
      differentiation of Th cells toward Th2 cells. However, little is known about the 
      effect of CD80 and CD86 knockdown on Th1/Th2 cytokine production in mature DCs 
      (mDCs). The aim of the present study was to investigate whether small-interfering 
      RNA (siRNA) could suppress the surface expression of CD80 and CD86 in mDCs. The 
      effects of CD80 and CD86 knockdown in mDCs on Th1/Th2 cytokine expression were 
      examined using an asthmatic murine model. DCs were isolated, separated and 
      cultured in vitro. Flow cytometry was used to examine the expression of CD11c, 
      CD80 and CD86 on the DCs. The DCs were transfected with CD80- and CD86-specific 
      siRNA, while non-siRNA and negative siRNA controls were also designed. Then, the 
      mRNA and protein expression levels of CD80 and CD86 were determined by reverse 
      transcription-quantitative polymerase chain reaction and flow cytometry, 
      respectively. The levels of interferon (IFN)-gamma and interleukin (IL)-4 produced by 
      T cells co-cultured with mDCs were measured by enzyme-linked immunosorbent assay. 
      Substantial downregulation of CD80 and CD86 mRNA and protein levels were observed 
      in the mDCs following transfection with siRNA. The level of IFN-gamma produced by T 
      cells co-cultured with mDCs was significantly increased in the siRNA group, while 
      IL-4 production was significantly decreased. These results show that specific 
      targeting of CD80 and CD86 with siRNA is able to suppress CD80/CD86 expression 
      and consequently regulate Th1/Th2 cytokine levels by increasing IFN-gamma production 
      and decreasing IL-4 levels in an asthmatic murine model.
FAU - Li, Jian-Guo
AU  - Li JG
AD  - Department of Respiratory Medicine, Sun Yat-Sen Memorial Hospital, Institute of 
      Respiratory Diseases, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. 
      China.
FAU - DU, Yu-Mo
AU  - DU YM
AD  - Department of Respiratory Medicine, Sun Yat-Sen Memorial Hospital, Institute of 
      Respiratory Diseases, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. 
      China.
FAU - Yan, Zhi-Dong
AU  - Yan ZD
AD  - Department of Respiratory Medicine, Sun Yat-Sen Memorial Hospital, Institute of 
      Respiratory Diseases, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. 
      China.
FAU - Yan, Jia
AU  - Yan J
AD  - Department of Intensive Care Unit, Tianjin Medical University General Hospital, 
      Tianjin 300052, P.R. China.
FAU - Zhuansun, Yong-Xun
AU  - Zhuansun YX
AD  - Department of Respiratory Medicine, Sun Yat-Sen Memorial Hospital, Institute of 
      Respiratory Diseases, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. 
      China.
FAU - Chen, Rui
AU  - Chen R
AD  - Department of Respiratory Medicine, Sun Yat-Sen Memorial Hospital, Institute of 
      Respiratory Diseases, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. 
      China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Respiratory Medicine, Sun Yat-Sen Memorial Hospital, Institute of 
      Respiratory Diseases, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. 
      China.
FAU - Feng, Su-Ling
AU  - Feng SL
AD  - Department of Respiratory Medicine, Sun Yat-Sen Memorial Hospital, Institute of 
      Respiratory Diseases, Sun Yat-Sen University, Guangzhou, Guangdong 510120, P.R. 
      China.
FAU - Ran, Pi-Xin
AU  - Ran PX
AD  - Department of Respiratory Medicine, The First Affiliated Hospital of Guangzhou 
      Medical College, Guangzhou, Guangdong 510120, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160113
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4774365
OTO - NOTNLM
OT  - CD80
OT  - CD86
OT  - Th1/Th2
OT  - asthma
OT  - dendritic cell
OT  - small-interfering RNA
EDAT- 2016/03/22 06:00
MHDA- 2016/03/22 06:01
PMCR- 2016/01/13
CRDT- 2016/03/22 06:00
PHST- 2014/11/06 00:00 [received]
PHST- 2015/12/14 00:00 [accepted]
PHST- 2016/03/22 06:00 [entrez]
PHST- 2016/03/22 06:00 [pubmed]
PHST- 2016/03/22 06:01 [medline]
PHST- 2016/01/13 00:00 [pmc-release]
AID - ETM-0-0-2989 [pii]
AID - 10.3892/etm.2016.2989 [doi]
PST - ppublish
SO  - Exp Ther Med. 2016 Mar;11(3):878-884. doi: 10.3892/etm.2016.2989. Epub 2016 Jan 
      13.