PMID- 26998028
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 11
IP  - 3
DP  - 2016 Mar
TI  - Rolipram stimulates angiogenesis and attenuates neuronal apoptosis through the 
      cAMP/cAMP-responsive element binding protein pathway following ischemic stroke in 
      rats.
PG  - 1005-1010
AB  - Rolipram, a phosphodiesterase-4 inhibitor, can activate the cyclic adenosine 
      monophosphate (cAMP)/cAMP-responsive element binding protein (CREB) pathway to 
      facilitate functional recovery following ischemic stroke. However, to date, the 
      effects of rolipram on angiogenesis and cerebral ischemia-induced neuronal 
      apoptosis are yet to be fully elucidated. In this study, the aim was to reveal 
      the effect of rolipram on the angiogenesis and neuronal apoptosis following brain 
      cerebral ischemia. Rat models of ischemic stroke were established following 
      transient middle cerebral artery occlusion and rolipram was administered for 
      three, seven and 14 days. The results were examined using behavioral tests, 
      triphenyl tetrazolium chloride staining, immunostaining and terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) to evaluate 
      the effects of rolipram therapy on functional outcome, angiogenesis and 
      apoptosis. Western blot analysis was used to show the phosphorylated- (p-)CREB 
      protein level in the ischemic hemisphere. The rolipram treatment group exhibited 
      a marked reduction in infarct size and modified neurological severity score 
      compared with the vehicle group, and rolipram treatment significantly promoted 
      the microvessel density in the ischemic boundary region and increased p-CREB 
      protein levels in the ischemic hemisphere. Furthermore, a significant reduction 
      in the number of TUNEL-positive cells was observed in the rolipram group compared 
      with the vehicle group. These findings suggest that rolipram has the ability to 
      attenuate cerebral ischemic injury, stimulate angiogenesis and reduce neuronal 
      apoptosis though the cAMP/CREB pathway.
FAU - Hu, Shouye
AU  - Hu S
AD  - Department of Osteonecrosis and Joint Reconstruction, Xi'an Honghui Hospital, 
      Xi'an, Shaanxi 710068, P.R. China; Xi'an Jiaotong University Health Science 
      Center, Xi'an, Shaanxi 710068, P.R. China.
FAU - Cao, Qingwen
AU  - Cao Q
AD  - Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710068, P.R. 
      China.
FAU - Xu, Peng
AU  - Xu P
AD  - Department of Osteonecrosis and Joint Reconstruction, Xi'an Honghui Hospital, 
      Xi'an, Shaanxi 710068, P.R. China; Xi'an Jiaotong University Health Science 
      Center, Xi'an, Shaanxi 710068, P.R. China.
FAU - Ji, Wenchen
AU  - Ji W
AD  - Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710068, P.R. 
      China.
FAU - Wang, Gang
AU  - Wang G
AD  - Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710068, P.R. 
      China; Department of Neurosurgery, Shaanxi Province People's Hospital, Xi'an, 
      Shaanxi 710068, P.R. China.
FAU - Zhang, Yuelin
AU  - Zhang Y
AD  - Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710068, P.R. 
      China; Department of Neurosurgery, Shaanxi Province People's Hospital, Xi'an, 
      Shaanxi 710068, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20151229
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4774329
OTO - NOTNLM
OT  - angiogenesis
OT  - cAMP/cAMP-responsive element binding protein pathway
OT  - neuronal apoptosis
OT  - rat
OT  - rolipram
EDAT- 2016/03/22 06:00
MHDA- 2016/03/22 06:01
PMCR- 2015/12/29
CRDT- 2016/03/22 06:00
PHST- 2014/02/04 00:00 [received]
PHST- 2014/06/13 00:00 [accepted]
PHST- 2016/03/22 06:00 [entrez]
PHST- 2016/03/22 06:00 [pubmed]
PHST- 2016/03/22 06:01 [medline]
PHST- 2015/12/29 00:00 [pmc-release]
AID - ETM-0-0-2958 [pii]
AID - 10.3892/etm.2015.2958 [doi]
PST - ppublish
SO  - Exp Ther Med. 2016 Mar;11(3):1005-1010. doi: 10.3892/etm.2015.2958. Epub 2015 Dec 
      29.