PMID- 26998092
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220317
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 11
IP  - 3
DP  - 2016 Mar
TI  - Analysis of the expression of SDF-1 splicing variants in human colorectal cancer 
      and normal mucosa tissues.
PG  - 1873-1878
AB  - C-X-C motif chemokine ligand 12 (CXCL12), also termed stromal cell-derived 
      factor-1 (SDF-1) is a small protein 8-14 kDa in length that is expressed as six 
      isoforms, consisting of SDF-1alpha, SDF-1beta, SDF-1gamma, SDF-1delta, SDF-1epsilon and SDF-1theta. All 
      six isoforms are encoded by the single CXCL12 gene on chromosome 10. This gene 
      regulates leukocyte trafficking and is variably expressed in a number of normal 
      and cancer tissues. The potential role of the novel CXCL12 splice variants as 
      components of the CXCR4 axis in cancer development is not fully understood. The 
      present study aimed to analyze the expression profile of the various SDF-1 
      isoforms and SDF-1 polymorphisms, and the association with the 
      clinicopathological features and overall survival of patients with colorectal 
      cancer (CRC). SDF-1 polymorphism analysis was performed using restriction 
      fragment length polymorphism (RFLP) analysis in 73 histologically confirmed human 
      CRC tissue samples at various stages of disease. The expression pattern of the 
      SDF-1 isoforms was analyzed by reverse transcription-polymerase chain reaction in 
      40 histologically confirmed human CRC tissue samples obtained at various stages 
      of disease, as well as in matched adjacent normal mucosa samples. The presence of 
      the CXCL12 gene polymorphism rs1801157 demonstrated an association with local 
      progression of the primary tumor, as indicated by the T stage. The frequency of 
      the GG genotype was slightly increased in patients with stage 3 and 4 tumors 
      (78.0%) compared with the incidence of the GA/AA genotype (69.5%; P=0.067). The 
      expression of SDF-1beta was associated with the presence of metastases (P=0.0656) 
      and the expression of SDF-1gamma was significantly associated with tumor size 
      (P=0.0423). The present study is the first to analyze the association between the 
      expression profile of the chemokine CXCL12 splice variants in human CRC tissues 
      and their clinical relevance. The present results reveal that the CXCL12 G801A 
      polymorphism is a low-penetrance risk factor for the development of CRC, and was 
      associated with the T stage. All six isoforms of SDF-1 were expressed in CRC 
      tissues. The expression of SDF-1beta was found to be associated with metastases and 
      SDF-1gamma appears to be a possible tumor marker for local tumor progression.
FAU - Allami, Risala Hussain
AU  - Allami RH
AD  - First Department of Internal Medicine, Johannes Gutenberg University Hospital of 
      Mainz, D-55130 Mainz, Germany.
FAU - Graf, Claudine
AU  - Graf C
AD  - Third Department of Internal Medicine, Johannes Gutenberg University Hospital of 
      Mainz, D-55130 Mainz, Germany.
FAU - Martchenko, Ksenia
AU  - Martchenko K
AD  - Department of Internal Medicine, Marienhospital Darmstadt, D-64285 Darmstadt, 
      Germany.
FAU - Voss, Beatrice
AU  - Voss B
AD  - Third Department of Internal Medicine, Johannes Gutenberg University Hospital of 
      Mainz, D-55130 Mainz, Germany.
FAU - Becker, Marc
AU  - Becker M
AD  - First Department of Internal Medicine, Johannes Gutenberg University Hospital of 
      Mainz, D-55130 Mainz, Germany.
FAU - Berger, Martin R
AU  - Berger MR
AD  - Research Group Toxicology and Chemotherapy, German Cancer Research Center, 
      D-69117 Heidelberg, Germany.
FAU - Galle, Peter R
AU  - Galle PR
AD  - First Department of Internal Medicine, Johannes Gutenberg University Hospital of 
      Mainz, D-55130 Mainz, Germany.
FAU - Theobald, Matthias
AU  - Theobald M
AD  - Third Department of Internal Medicine, Johannes Gutenberg University Hospital of 
      Mainz, D-55130 Mainz, Germany.
FAU - Wehler, Thomas C
AU  - Wehler TC
AD  - Third Department of Internal Medicine, Johannes Gutenberg University Hospital of 
      Mainz, D-55130 Mainz, Germany.
FAU - Schimanski, Carl C
AU  - Schimanski CC
AD  - Department of Internal Medicine, Marienhospital Darmstadt, D-64285 Darmstadt, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20160125
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC4774538
OTO - NOTNLM
OT  - C-X-C motif chemokine ligand 12
OT  - colorectal cancer
OT  - isoforms
OT  - stromal cell derived factor 1 splice variants
EDAT- 2016/03/22 06:00
MHDA- 2016/03/22 06:01
PMCR- 2016/01/25
CRDT- 2016/03/22 06:00
PHST- 2014/05/28 00:00 [received]
PHST- 2015/06/22 00:00 [accepted]
PHST- 2016/03/22 06:00 [entrez]
PHST- 2016/03/22 06:00 [pubmed]
PHST- 2016/03/22 06:01 [medline]
PHST- 2016/01/25 00:00 [pmc-release]
AID - OL-0-0-4139 [pii]
AID - 10.3892/ol.2016.4139 [doi]
PST - ppublish
SO  - Oncol Lett. 2016 Mar;11(3):1873-1878. doi: 10.3892/ol.2016.4139. Epub 2016 Jan 
      25.