PMID- 26998099
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 11
IP  - 3
DP  - 2016 Mar
TI  - CXCL10 and CCL2 mRNA expression in monocytes is inversely correlated with the 
      HLA-DR lower fraction of monocytes in patients with renal cell carcinoma.
PG  - 1911-1916
AB  - Circulating cluster of differentiation (CD)14+ human leukocyte antigen 
      (HLA)-DRlow/- monocytes, those with a lower HLA-DR expression or are negative for 
      HLA-DR, are considered to be involved in systemic immunosuppression in patients 
      with several malignant tumors. However, few studies have investigated in detail 
      the gene expression profile of CD14+HLA-DRlow/- monocytes. In the present study, 
      the mRNA expression levels of immune-associated molecules in CD14+ monocytes 
      isolated from healthy donors and patients with renal cell carcinoma (RCC) were 
      analyzed. Consistent with previous studies, the percentage of HLA-DRlow/- cells 
      in CD14+ monocytes was significantly increased in patients with RCC compared with 
      healthy donors. In 3 of the 4 patients who underwent surgical resection of the 
      primary tumor, the percentage of CD14+HLA-DRlow/- cells was significantly 
      decreased following surgery. The mRNA expression levels of cyclooxygenase 2, 
      transforming growth factor beta, interleukin 6R, chemokine (C-C motif) ligand 2 
      (CCL2), chemokine (C-X-C motif) ligand 10 (CXCL10), oncostatin M, and vascular 
      endothelial growth factor-A in CD14+ monocytes were quantified using reverse 
      transcription-quantitative polymerase chain reaction. The results of the present 
      study revealed that increased expression levels of CCL2 and CXCL10 were inversely 
      correlated with the percentage of CD14+HLA-DRlow/- monocytes. This suggested that 
      monocytes in RCC patients were immunologically suppressed, and that 
      immunosuppression in RCC patients may be due, in part, to the dysfunction of 
      circulating monocytes.
FAU - Motoshima, Takanobu
AU  - Motoshima T
AD  - Department of Urology, Graduate School of Medical Sciences, Kumamoto University, 
      Kumamoto 860-8556, Japan; Department of Cell Pathology, Graduate School of 
      Medical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
FAU - Komohara, Yoshihiro
AU  - Komohara Y
AD  - Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto 
      University, Kumamoto 860-8556, Japan.
FAU - Horlad, Hasita
AU  - Horlad H
AD  - Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto 
      University, Kumamoto 860-8556, Japan.
FAU - Tsukamoto, Hirotake
AU  - Tsukamoto H
AD  - Department of Immunogenetics, Graduate School of Medical Sciences, Kumamoto 
      University, Kumamoto 860-8556, Japan.
FAU - Fujita, Mitsugu
AU  - Fujita M
AD  - Department of Microbiology, Kinki University Faculty of Medicine, Osaka 542-0073, 
      Japan.
FAU - Saito, Yoichi
AU  - Saito Y
AD  - Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto 
      University, Kumamoto 860-8556, Japan.
FAU - Tanoue, Kenichiro
AU  - Tanoue K
AD  - Department of Urology, Graduate School of Medical Sciences, Kumamoto University, 
      Kumamoto 860-8556, Japan.
FAU - Kasejima, Yutaka
AU  - Kasejima Y
AD  - Department of Urology, Graduate School of Medical Sciences, Kumamoto University, 
      Kumamoto 860-8556, Japan.
FAU - Sugiyama, Yutaka
AU  - Sugiyama Y
AD  - Department of Urology, Graduate School of Medical Sciences, Kumamoto University, 
      Kumamoto 860-8556, Japan.
FAU - Kawano, Yoshiaki
AU  - Kawano Y
AD  - Department of Urology, Graduate School of Medical Sciences, Kumamoto University, 
      Kumamoto 860-8556, Japan.
FAU - Nishimura, Yasuharu
AU  - Nishimura Y
AD  - Department of Immunogenetics, Graduate School of Medical Sciences, Kumamoto 
      University, Kumamoto 860-8556, Japan.
FAU - Takeya, Motohiro
AU  - Takeya M
AD  - Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto 
      University, Kumamoto 860-8556, Japan.
FAU - Eto, Masatoshi
AU  - Eto M
AD  - Department of Urology, Graduate School of Medical Sciences, Kumamoto University, 
      Kumamoto 860-8556, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160120
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC4774459
OTO - NOTNLM
OT  - chemokine (C-C motif) ligand 2
OT  - chemokine (C-X-C motif) ligand 10
OT  - cluster of differentiation 14
OT  - myeloid-derived suppressor cells
OT  - renal cell carcinoma
EDAT- 2016/03/22 06:00
MHDA- 2016/03/22 06:01
PMCR- 2016/01/20
CRDT- 2016/03/22 06:00
PHST- 2014/12/03 00:00 [received]
PHST- 2015/12/18 00:00 [accepted]
PHST- 2016/03/22 06:00 [entrez]
PHST- 2016/03/22 06:00 [pubmed]
PHST- 2016/03/22 06:01 [medline]
PHST- 2016/01/20 00:00 [pmc-release]
AID - OL-0-0-4132 [pii]
AID - 10.3892/ol.2016.4132 [doi]
PST - ppublish
SO  - Oncol Lett. 2016 Mar;11(3):1911-1916. doi: 10.3892/ol.2016.4132. Epub 2016 Jan 
      20.