PMID- 26999337
OWN - NLM
STAT- MEDLINE
DCOM- 20161221
LR  - 20161230
IS  - 1533-0311 (Electronic)
IS  - 0193-1091 (Linking)
VI  - 38
IP  - 4
DP  - 2016 Apr
TI  - Fluorescence In Situ Hybridization for Melanoma Diagnosis: A Review and a 
      Reappraisal.
PG  - 253-69
LID - 10.1097/DAD.0000000000000380 [doi]
AB  - Although conventional histopathological examination is the undisputable mainstay 
      for the diagnosis of melanocytic skin neoplasms, fluorescence in situ 
      hybridization (FISH) has the potential to provide important information to 
      morphologically challenging cases. The standard melanoma FISH test targeting 
      RREB1 (6p25), MYB (6q23), CCND1 (11q13), and centromere 6 is an effective 
      compromise between cost, technical complexity, and sensitivity. The authors use 
      the standard FISH-positivity as a tie-breaker for challenging melanocytic 
      neoplasms mainly in a non-Spitzoid morphologic context because the currently 
      available test leaves several unresolved issues: namely, a relatively low 
      diagnostic accuracy in morphologically ambiguous melanocytic neoplasms; a 
      relatively low sensitivity and specificity in Spitzoid neoplasms; and the 
      occurrence of false positives due to tetraploidy in Spitz nevi and in nevi with 
      an atypical epithelioid component. Under investigation is currently a new 
      melanoma probe cocktail targeting RREB1 (6p25), C-MYC (8q24), CDKN2A (9p21), and 
      CCND1 (11q13). However, CDKN2A is a significant parameter only if lost in 
      homozygosis, and this complicates the interpretation of the results. Furthermore, 
      the new melanoma probe cocktail has been tested on cases of atypical Spitzoid 
      proliferations with fatal outcomes which at present are too few to allow definite 
      conclusions. The authors propose the implementation of a FISH algorithm (standard 
      4-probe test followed by either C-MYC or CDKN2A/centromere 9) to assist the 
      histopathological diagnosis and minimize the technical problems. Nevertheless, 
      because the diagnostic accuracy of the FISH technique is far from being absolute, 
      the overall clinicopathological context must always guide the decision-making 
      process about the management of morphobiologically ambiguous melanocytic 
      proliferations.
FAU - Ferrara, Gerardo
AU  - Ferrara G
AD  - GF is a Consultant Histopathologist and Dermatopathologist, ACDV is the Head of 
      the Molecular Biology Lab, Department of Oncology, Anatomic Pathology Unit, 
      Gaetano Rummo General Hospital, Benevento, Italy.
FAU - De Vanna, Anna Chiara
AU  - De Vanna AC
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Am J Dermatopathol
JT  - The American Journal of dermatopathology
JID - 7911005
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Melanoma/*diagnosis/*genetics
MH  - Skin Neoplasms/*diagnosis/*genetics
EDAT- 2016/03/22 06:00
MHDA- 2016/12/22 06:00
CRDT- 2016/03/22 06:00
PHST- 2016/03/22 06:00 [entrez]
PHST- 2016/03/22 06:00 [pubmed]
PHST- 2016/12/22 06:00 [medline]
AID - 00000372-201604000-00001 [pii]
AID - 10.1097/DAD.0000000000000380 [doi]
PST - ppublish
SO  - Am J Dermatopathol. 2016 Apr;38(4):253-69. doi: 10.1097/DAD.0000000000000380.