PMID- 26999959 OWN - NLM STAT- MEDLINE DCOM- 20160505 LR - 20191113 IS - 1733-1331 (Print) IS - 1733-1331 (Linking) VI - 64 IP - 4 DP - 2015 TI - Molecular Methods for Identification of Monophasic Salmonella Typhimurium Strains. PG - 383-6 AB - Two molecular biology methods were used to differentiate Salmonella enterica 1,4,[5],12:i:- strains: "Salmonella Check&Trace microarray" (CT) and multiplex PCR (mPCR). For 92 strains in CT result "Salmonella 1,4,[5],12:i:-" were obtained. Those strains were confirmed in mPCR as monophasic fljB-lack Salmonella Typhimurium. For 17 strains, which in CT assay were recognized as Salmonella Typhimurium, the same identification was obtained in mPCR. Reference Salmonella strains: Lagos, Agama, Tsevie, Glocester and Tumodi in CT were recognized as Salmonella genovar, in mPCR--as Salmonella O:4, H:i other than Salmonella Typhimurium, the same like Salmonella Farsta, recognized incorrectly in CT as Salmonella Typhimurium. FAU - Madajczak, Grzegorz AU - Madajczak G FAU - Dera-Tomaszewska, Bozena AU - Dera-Tomaszewska B FAU - Wasiak, Monika AU - Wasiak M FAU - Chrost, Anna AU - Chrost A FAU - Szych, Jolanta AU - Szych J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Poland TA - Pol J Microbiol JT - Polish journal of microbiology JID - 101229003 RN - 0 (Antigens, Bacterial) RN - 0 (Bacterial Proteins) RN - 0 (DNA, Bacterial) SB - IM MH - Antigens, Bacterial MH - Bacterial Proteins/*genetics MH - DNA, Bacterial/genetics MH - Multiplex Polymerase Chain Reaction/*methods MH - Protein Array Analysis/*methods MH - Salmonella typhimurium/classification/*genetics MH - Serogroup EDAT- 2015/01/01 00:00 MHDA- 2016/05/06 06:00 CRDT- 2016/03/23 06:00 PHST- 2016/03/23 06:00 [entrez] PHST- 2015/01/01 00:00 [pubmed] PHST- 2016/05/06 06:00 [medline] AID - 10.5604/17331331.1185238 [doi] PST - ppublish SO - Pol J Microbiol. 2015;64(4):383-6. doi: 10.5604/17331331.1185238.