PMID- 27000098
OWN - NLM
STAT- MEDLINE
DCOM- 20171211
LR  - 20180129
IS  - 2047-4881 (Electronic)
IS  - 2047-4873 (Linking)
VI  - 23
IP  - 13
DP  - 2016 Sep
TI  - Does exercise training impact clock genes in patients with coronary artery 
      disease and type 2 diabetes mellitus?
PG  - 1375-82
LID - 10.1177/2047487316639682 [doi]
AB  - BACKGROUND: Recent findings revealed negative effects of deregulated molecular 
      circadian rhythm in coronary artery disease (CAD) and type 2 diabetes mellitus 
      (T2DM). Physical exercise training (ET) has been shown to promote anti-diabetic 
      and anti-atherogenic responses in skeletal muscle of these patients, but the role 
      of the circadian clock-machinery remains unknown. This study investigated whether 
      mRNA expression of clock genes in skeletal muscle of CAD and T2DM patients is 
      influenced by physical ET intervention. METHODS: Nineteen patients with CAD and 
      T2DM (age 64 +/- 5 years) were randomised to either six months of ET (four weeks of 
      in-hospital ET followed by a five-month ambulatory programme) or usual care. At 
      the beginning of the study, after four weeks and after six months parameters of 
      metabolic and cardiovascular risk factors, and physical exercise capacity were 
      assessed. Gene expression was measured in skeletal muscle biopsies by 
      quantitative real-time polymerase chain reaction (PCR). RESULTS: A selection of 
      clock genes and associated components (circadian locomoter output cycle kaput 
      protein (CLOCK), period (PER) 1, cryptochrome (CRY) 2 and 
      aminolevulinate-deltA-synthase-1 (ALAS1)) was reliably measured and used for 
      further analysis. A time-dependent effect in gene expression was observed in 
      CLOCK (p = 0.013) and a significant interaction between time and intervention was 
      observed for ALAS1 (p = 0.032; p = 0.014) as a result of ET. CONCLUSION: This is 
      the first study to analyse clock gene expression in skeletal muscles of patients 
      with CAD and T2DM participating in a long-lasting exercise intervention. ET, as 
      one of the cornerstones in prevention and rehabilitation of CAD and T2DM, exerts 
      no effects on CLOCK genes but meaningful effects on the clock-associated gene 
      ALAS1.
CI  - (c) The European Society of Cardiology 2016.
FAU - Steidle-Kloc, Eva
AU  - Steidle-Kloc E
AD  - University Institute of Sports Medicine, Prevention and Rehabilitation, 
      Paracelsus Medical University, Austria.
FAU - Schonfelder, Martin
AU  - Schonfelder M
AD  - Research Institute of Molecular Sports Medicine and Rehabilitation, Paracelsus 
      Medical University, Austria.
FAU - Muller, Edith
AU  - Muller E
AD  - Research Institute of Molecular Sports Medicine and Rehabilitation, Paracelsus 
      Medical University, Austria.
FAU - Sixt, Sebastian
AU  - Sixt S
AD  - Angiologikum GmbH, Hamburg, Germany.
FAU - Schuler, Gerhard
AU  - Schuler G
AD  - Heart Center, Department of Cardiology, University of Leipzig, Germany.
FAU - Patsch, Wolfgang
AU  - Patsch W
AD  - Institute of Pharmacology and Toxicology, Paracelsus Medical University, 
      Salzburg, Austria.
FAU - Niebauer, Josef
AU  - Niebauer J
AD  - University Institute of Sports Medicine, Prevention and Rehabilitation, 
      Paracelsus Medical University, Austria Research Institute of Molecular Sports 
      Medicine and Rehabilitation, Paracelsus Medical University, Austria 
      j.niebauer@salk.at.
LA  - eng
PT  - Journal Article
DEP - 20160321
PL  - England
TA  - Eur J Prev Cardiol
JT  - European journal of preventive cardiology
JID - 101564430
RN  - 0 (Muscle Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
CIN - Eur J Prev Cardiol. 2016 Sep;23(13):1372-4. PMID: 27231397
MH  - Circadian Rhythm/*genetics
MH  - Coronary Artery Disease/complications/*genetics/rehabilitation
MH  - Diabetes Mellitus, Type 2/complications/*genetics/rehabilitation
MH  - Exercise/*physiology
MH  - *Exercise Therapy
MH  - Female
MH  - *Gene Expression
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Muscle Proteins/biosynthesis/*genetics
MH  - Muscle, Skeletal/metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - Risk Factors
OTO - NOTNLM
OT  - Cardiovascular disease
OT  - cardiovascular risk
OT  - circadian rhythm
OT  - physical training
EDAT- 2016/03/24 06:00
MHDA- 2017/12/12 06:00
CRDT- 2016/03/23 06:00
PHST- 2015/08/17 00:00 [received]
PHST- 2016/02/27 00:00 [accepted]
PHST- 2016/03/23 06:00 [entrez]
PHST- 2016/03/24 06:00 [pubmed]
PHST- 2017/12/12 06:00 [medline]
AID - 2047487316639682 [pii]
AID - 10.1177/2047487316639682 [doi]
PST - ppublish
SO  - Eur J Prev Cardiol. 2016 Sep;23(13):1375-82. doi: 10.1177/2047487316639682. Epub 
      2016 Mar 21.