PMID- 27005792
OWN - NLM
STAT- MEDLINE
DCOM- 20170130
LR  - 20181202
IS  - 1873-2976 (Electronic)
IS  - 0960-8524 (Linking)
VI  - 217
DP  - 2016 Oct
TI  - Microbial biodegradation of proteinaceous tannery solid waste and production of a 
      novel value added product - Metalloprotease.
PG  - 150-6
LID - S0960-8524(16)30319-4 [pii]
LID - 10.1016/j.biortech.2016.03.033 [doi]
AB  - In this study, animal fleshing (ANFL) was utilized as a substrate for the 
      production of extracellular protease by Clostridium limosum through central 
      composite rotatable design (CCRD) and response surface methodology (RSM). Optimum 
      protease production of 433U/ml was achieved and the purified enzyme was 
      identified as acidic metalloprotease, a monomeric protein. The molecular weight 
      of the enzyme was 71kDa, whose activity was enhanced by bivalent metals such as 
      Zn(2+) and Mg(2+). Scanning electron microscopy (SEM) examination also revealed 
      the hydrolysis/microbial degradation of ANFL through protease activity in the 
      anaerobic fermentation process. Simultaneous hydrolysis of ANFL and production of 
      an enzyme with the potential for different industrial applications provide an 
      attractive methodology for the disposal of tannery solid waste.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Ravindran, Balasubramani
AU  - Ravindran B
AD  - Animal Environment Division, Department of Animal Biotechnology and Environment, 
      National Institute of Animal Science, RDA, Jeonju, South Korea. Electronic 
      address: kalamravi@gmail.com.
FAU - Wong, Jonathan W C
AU  - Wong JW
AD  - Sino-Forest Applied Research Centre for Pearl River Delta Environment, and 
      Department of Biology, Hong Kong Baptist University, Hong Kong Special 
      Administrative Region.
FAU - Selvam, Ammaiyappan
AU  - Selvam A
AD  - Sino-Forest Applied Research Centre for Pearl River Delta Environment, and 
      Department of Biology, Hong Kong Baptist University, Hong Kong Special 
      Administrative Region.
FAU - Thirunavukarasu, Kathirvel
AU  - Thirunavukarasu K
AD  - Department of Biomedical Engineering, Sathyabama University, Chennai, India.
FAU - Sekaran, Ganesan
AU  - Sekaran G
AD  - Environmental Technology Division, Central Leather Research Institute, Chennai, 
      India.
LA  - eng
PT  - Journal Article
DEP - 20160310
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (Amino Acids)
RN  - 0 (Solid Waste)
RN  - EC 3.4.- (Metalloproteases)
SB  - IM
MH  - Amino Acids/analysis
MH  - Analysis of Variance
MH  - Animals
MH  - Biodegradation, Environmental/drug effects
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Extracellular Space/metabolism
MH  - Fermentation/drug effects
MH  - Hydrogen-Ion Concentration
MH  - Hydrolysis
MH  - Metalloproteases/isolation & purification/*metabolism
MH  - Microscopy, Electron, Scanning
MH  - Models, Theoretical
MH  - Molecular Weight
MH  - Reproducibility of Results
MH  - Solid Waste/*analysis
MH  - *Tanning
MH  - Temperature
MH  - Time Factors
OTO - NOTNLM
OT  - Acid protease
OT  - Clostridium limosum
OT  - Fermentation
OT  - Response surface methodology
OT  - Tannery solid waste
EDAT- 2016/03/24 06:00
MHDA- 2017/01/31 06:00
CRDT- 2016/03/24 06:00
PHST- 2015/12/30 00:00 [received]
PHST- 2016/03/03 00:00 [revised]
PHST- 2016/03/04 00:00 [accepted]
PHST- 2016/03/24 06:00 [entrez]
PHST- 2016/03/24 06:00 [pubmed]
PHST- 2017/01/31 06:00 [medline]
AID - S0960-8524(16)30319-4 [pii]
AID - 10.1016/j.biortech.2016.03.033 [doi]
PST - ppublish
SO  - Bioresour Technol. 2016 Oct;217:150-6. doi: 10.1016/j.biortech.2016.03.033. Epub 
      2016 Mar 10.