PMID- 27007252
OWN - NLM
STAT- MEDLINE
DCOM- 20160826
LR  - 20190202
IS  - 1553-7374 (Electronic)
IS  - 1553-7366 (Print)
IS  - 1553-7366 (Linking)
VI  - 12
IP  - 3
DP  - 2016 Mar
TI  - Activation of Plant Innate Immunity by Extracellular High Mobility Group Box 3 
      and Its Inhibition by Salicylic Acid.
PG  - e1005518
LID - 10.1371/journal.ppat.1005518 [doi]
LID - e1005518
AB  - Damage-associated molecular pattern molecules (DAMPs) signal the presence of 
      tissue damage to induce immune responses in plants and animals. Here, we report 
      that High Mobility Group Box 3 (HMGB3) is a novel plant DAMP. Extracellular 
      HMGB3, through receptor-like kinases BAK1 and BKK1, induced hallmark innate 
      immune responses, including i) MAPK activation, ii) defense-related gene 
      expression, iii) callose deposition, and iv) enhanced resistance to Botrytis 
      cinerea. Infection by necrotrophic B. cinerea released HMGB3 into the 
      extracellular space (apoplast). Silencing HMGBs enhanced susceptibility to B. 
      cinerea, while HMGB3 injection into apoplast restored resistance. Like its human 
      counterpart, HMGB3 binds salicylic acid (SA), which results in inhibition of its 
      DAMP activity. An SA-binding site mutant of HMGB3 retained its DAMP activity, 
      which was no longer inhibited by SA, consistent with its reduced SA-binding 
      activity. These results provide cross-kingdom evidence that HMGB proteins 
      function as DAMPs and that SA is their conserved inhibitor.
FAU - Choi, Hyong Woo
AU  - Choi HW
AD  - Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of 
      America.
FAU - Manohar, Murli
AU  - Manohar M
AD  - Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of 
      America.
FAU - Manosalva, Patricia
AU  - Manosalva P
AD  - Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of 
      America.
FAU - Tian, Miaoying
AU  - Tian M
AD  - Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of 
      America.
FAU - Moreau, Magali
AU  - Moreau M
AD  - Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of 
      America.
FAU - Klessig, Daniel F
AU  - Klessig DF
AD  - Boyce Thompson Institute for Plant Research, Ithaca, New York, United States of 
      America.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160323
PL  - United States
TA  - PLoS Pathog
JT  - PLoS pathogens
JID - 101238921
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Cyclopentanes)
RN  - 0 (Ethylenes)
RN  - 0 (Oxylipins)
RN  - O414PZ4LPZ (Salicylic Acid)
SB  - IM
MH  - Arabidopsis/genetics
MH  - Arabidopsis Proteins/genetics/metabolism
MH  - Botrytis/*immunology/metabolism
MH  - Cyclopentanes/metabolism
MH  - Disease Resistance
MH  - Ethylenes/metabolism
MH  - *Gene Expression Regulation, Plant
MH  - Oxylipins/*metabolism
MH  - Plant Diseases/*parasitology
MH  - Plant Leaves/genetics
MH  - Plants/*immunology
MH  - Pseudomonas syringae/metabolism
MH  - Salicylic Acid/*metabolism
MH  - Signal Transduction/drug effects
PMC - PMC4805298
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/03/24 06:00
MHDA- 2016/08/27 06:00
PMCR- 2016/03/23
CRDT- 2016/03/24 06:00
PHST- 2015/09/09 00:00 [received]
PHST- 2016/03/01 00:00 [accepted]
PHST- 2016/03/24 06:00 [entrez]
PHST- 2016/03/24 06:00 [pubmed]
PHST- 2016/08/27 06:00 [medline]
PHST- 2016/03/23 00:00 [pmc-release]
AID - PPATHOGENS-D-15-02146 [pii]
AID - 10.1371/journal.ppat.1005518 [doi]
PST - epublish
SO  - PLoS Pathog. 2016 Mar 23;12(3):e1005518. doi: 10.1371/journal.ppat.1005518. 
      eCollection 2016 Mar.