PMID- 27010387
OWN - NLM
STAT- MEDLINE
DCOM- 20170222
LR  - 20191210
IS  - 1573-6776 (Electronic)
IS  - 0141-5492 (Linking)
VI  - 38
IP  - 7
DP  - 2016 Jul
TI  - Intracellularly expressed nanobodies against non-structural protein 4 of porcine 
      reproductive and respiratory syndrome virus inhibit virus replication.
PG  - 1081-8
LID - 10.1007/s10529-016-2086-3 [doi]
AB  - OBJECTIVE: To isolate specific nanobodies to porcine reproductive and respiratory 
      syndrome virus (PRRSV) non-structural protein 4 (Nsp4) and investigate their 
      potential antiviral activities. RESULTS: Three PRRSV Nsp4-specific nanobodies 
      were isolated from a phage display library of the variable domains of camelid 
      heavy chain-only antibodies. Nanobody genes were introduced into MARC-145 cells 
      using lentivirus vectors to establish cell lines stably expressing nanobodies. 
      These intracellularly expressed nanobodies were tested for interaction with 
      PRRSV-encoded Nsp4 within PRRSV-infected MARC-145 cells. Nb41 and Nb43 
      intrabodies each potently inhibited PRRSV replication, protected MARC-145 cells 
      from PRRSV-induced cytopathic effect and fully blocked PRRSV replication at an 
      MOI of 0.001 or lower. CONCLUSION: Intracellularly expressed Nb41 and Nb43 
      potently suppressed PRRSV replication in MARC-145 cells. Nanobodies hold great 
      potential for development as novel antiviral treatments for PRRSV infection.
FAU - Liu, Hongliang
AU  - Liu H
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, 712100, Shaanxi, China.
FAU - Liang, Chao
AU  - Liang C
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, 712100, Shaanxi, China.
FAU - Duan, Hong
AU  - Duan H
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, 712100, Shaanxi, China.
FAU - Zhang, Xiaobin
AU  - Zhang X
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, 712100, Shaanxi, China.
FAU - Wang, Xiangpeng
AU  - Wang X
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, 712100, Shaanxi, China.
FAU - Xiao, Shuqi
AU  - Xiao S
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, 712100, Shaanxi, China.
FAU - Zhou, En-Min
AU  - Zhou EM
AUID- ORCID: 0000-0001-8942-013X
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, 712100, Shaanxi, China. zhouem@nwsuaf.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160324
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (Antiviral Agents)
RN  - 0 (Single-Domain Antibodies)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/immunology/*pharmacology
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects
MH  - Single-Domain Antibodies/immunology/*pharmacology
MH  - Viral Nonstructural Proteins/*immunology
MH  - Virus Replication/*drug effects
OTO - NOTNLM
OT  - Intrabody
OT  - Lentivirus
OT  - Nanobody
OT  - Non-structural protein 4
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Single-domain antibody
EDAT- 2016/03/25 06:00
MHDA- 2017/02/23 06:00
CRDT- 2016/03/25 06:00
PHST- 2016/01/28 00:00 [received]
PHST- 2016/03/17 00:00 [accepted]
PHST- 2016/03/25 06:00 [entrez]
PHST- 2016/03/25 06:00 [pubmed]
PHST- 2017/02/23 06:00 [medline]
AID - 10.1007/s10529-016-2086-3 [pii]
AID - 10.1007/s10529-016-2086-3 [doi]
PST - ppublish
SO  - Biotechnol Lett. 2016 Jul;38(7):1081-8. doi: 10.1007/s10529-016-2086-3. Epub 2016 
      Mar 24.