PMID- 27010400
OWN - NLM
STAT- MEDLINE
DCOM- 20160811
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 3
DP  - 2016
TI  - Microparticle-Induced Coagulation Relates to Coronary Artery Atherosclerosis in 
      Severe Aortic Valve Stenosis.
PG  - e0151499
LID - 10.1371/journal.pone.0151499 [doi]
LID - e0151499
AB  - BACKGROUND: Circulating microparticles (MPs) derived from endothelial cells and 
      blood cells bear procoagulant activity and promote thrombin generation. Thrombin 
      exerts proinflammatory effects mediating the progression of atherosclerosis. 
      Aortic valve stenosis may represent an atherosclerosis-like process involving 
      both the aortic valve and the vascular system. The aim of this study was to 
      investigate whether MP-induced thrombin generation is related to coronary 
      atherosclerosis and aortic valve calcification. METHODS: In a cross-sectional 
      study of 55 patients with severe aortic valve stenosis, we assessed the coronary 
      calcification score (CAC) as indicator of total coronary atherosclerosis burden, 
      and aortic valve calcification (AVC) by computed tomography. 
      Thrombin-antithrombin complex (TATc) levels were measured as a marker for 
      thrombin formation. Circulating MPs were characterized by flow cytometry 
      according to the expression of established surface antigens and by measuring 
      MP-induced thrombin generation. RESULTS: Patients with CAC score below the median 
      were classified as patients with low CAC, patients with CAC Score above the 
      median as high CAC. In patients with high CAC compared to patients with low CAC 
      we detected higher levels of TATc, platelet-derived MPs (PMPs), 
      endothelial-derived MPs (EMPs) and MP-induced thrombin generation. Increased 
      level of PMPs and MP-induced thrombin generation were independent predictors for 
      the severity of CAC. In contrast, AVC Score did not differ between patients with 
      high and low CAC and did neither correlate with MPs levels nor with MP-induced 
      thrombin generation. CONCLUSION: In patients with severe aortic valve stenosis 
      MP-induced thrombin generation was independently associated with the severity of 
      CAC but not AVC indicating different pathomechanisms involved in coronary artery 
      and aortic valve calcification.
FAU - Horn, Patrick
AU  - Horn P
AD  - Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, 
      University Dusseldorf, Dusseldorf, Germany.
FAU - Erkilet, Gulsum
AU  - Erkilet G
AD  - Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, 
      University Dusseldorf, Dusseldorf, Germany.
FAU - Veulemans, Verena
AU  - Veulemans V
AD  - Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, 
      University Dusseldorf, Dusseldorf, Germany.
FAU - Kropil, Patric
AU  - Kropil P
AD  - Department of Diagnostic and Interventional Radiology, Medical Faculty, 
      University Dusseldorf, Dusseldorf, Germany.
FAU - Schurgers, Leon
AU  - Schurgers L
AD  - Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), 
      Maastricht University, Maastricht, The Netherlands.
FAU - Zeus, Tobias
AU  - Zeus T
AD  - Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, 
      University Dusseldorf, Dusseldorf, Germany.
FAU - Heiss, Christian
AU  - Heiss C
AD  - Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, 
      University Dusseldorf, Dusseldorf, Germany.
FAU - Kelm, Malte
AU  - Kelm M
AD  - Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, 
      University Dusseldorf, Dusseldorf, Germany.
AD  - Cardiovascular Research Institute Dusseldorf (CARID), Medical Faculty, University 
      Dusseldorf, Dusseldorf, Germany.
FAU - Westenfeld, Ralf
AU  - Westenfeld R
AD  - Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, 
      University Dusseldorf, Dusseldorf, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160324
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (antithrombin III-protease complex)
RN  - 9000-94-6 (Antithrombin III)
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - EC 3.4.21.5 (Thrombin)
RN  - Aortic Valve, Calcification of
SB  - IM
MH  - Antithrombin III
MH  - Aortic Valve/*pathology
MH  - Aortic Valve Stenosis/blood/*pathology
MH  - Blood Coagulation
MH  - Calcinosis/blood/*pathology
MH  - Cell-Derived Microparticles/*pathology
MH  - Coronary Artery Disease/blood/*pathology
MH  - Coronary Vessels/*pathology
MH  - Cross-Sectional Studies
MH  - Female
MH  - Humans
MH  - Male
MH  - Peptide Hydrolases/blood
MH  - Thrombin/analysis
PMC - PMC4807100
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/03/25 06:00
MHDA- 2016/08/12 06:00
PMCR- 2016/03/24
CRDT- 2016/03/25 06:00
PHST- 2015/11/25 00:00 [received]
PHST- 2016/02/29 00:00 [accepted]
PHST- 2016/03/25 06:00 [entrez]
PHST- 2016/03/25 06:00 [pubmed]
PHST- 2016/08/12 06:00 [medline]
PHST- 2016/03/24 00:00 [pmc-release]
AID - PONE-D-15-51352 [pii]
AID - 10.1371/journal.pone.0151499 [doi]
PST - epublish
SO  - PLoS One. 2016 Mar 24;11(3):e0151499. doi: 10.1371/journal.pone.0151499. 
      eCollection 2016.