PMID- 27011354
OWN - NLM
STAT- MEDLINE
DCOM- 20171113
LR  - 20231213
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 5
DP  - 2016 Mar 24
TI  - Sensitive red protein calcium indicators for imaging neural activity.
LID - e12727 [pii]
LID - 10.7554/eLife.12727 [doi]
AB  - Genetically encoded calcium indicators (GECIs) allow measurement of activity in 
      large populations of neurons and in small neuronal compartments, over times of 
      milliseconds to months. Although GFP-based GECIs are widely used for in vivo 
      neurophysiology, GECIs with red-shifted excitation and emission spectra have 
      advantages for in vivo imaging because of reduced scattering and absorption in 
      tissue, and a consequent reduction in phototoxicity. However, current red GECIs 
      are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting 
      and quantifying neural activity. Here we present improved red GECIs based on 
      mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. 
      We characterized the performance of the new red GECIs in cultured neurons and in 
      mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate 
      deep-tissue imaging, dual-color imaging together with GFP-based reporters, and 
      the use of optogenetics in combination with calcium imaging.
FAU - Dana, Hod
AU  - Dana H
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Mohar, Boaz
AU  - Mohar B
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
AD  - Weizmann Institute of Science, Rehovot, Israel.
FAU - Sun, Yi
AU  - Sun Y
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Narayan, Sujatha
AU  - Narayan S
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Gordus, Andrew
AU  - Gordus A
AD  - Howard Hughes Medical Institute, The Rockefeller University, New York, United 
      States.
FAU - Hasseman, Jeremy P
AU  - Hasseman JP
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Tsegaye, Getahun
AU  - Tsegaye G
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Holt, Graham T
AU  - Holt GT
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Hu, Amy
AU  - Hu A
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Walpita, Deepika
AU  - Walpita D
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Patel, Ronak
AU  - Patel R
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Macklin, John J
AU  - Macklin JJ
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Bargmann, Cornelia I
AU  - Bargmann CI
AUID- ORCID: 0000-0002-8484-0618
AD  - Howard Hughes Medical Institute, The Rockefeller University, New York, United 
      States.
FAU - Ahrens, Misha B
AU  - Ahrens MB
AUID- ORCID: 0000-0002-3457-4462
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Schreiter, Eric R
AU  - Schreiter ER
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Jayaraman, Vivek
AU  - Jayaraman V
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Looger, Loren L
AU  - Looger LL
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Svoboda, Karel
AU  - Svoboda K
AUID- ORCID: 0000-0002-6670-7362
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
FAU - Kim, Douglas S
AU  - Kim DS
AUID- ORCID: 0000-0001-9029-314X
AD  - Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160324
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
RN  - 0 (Luminescent Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Biosensing Techniques/*methods
MH  - Caenorhabditis elegans
MH  - Calcium/*analysis
MH  - Cells, Cultured
MH  - Drosophila
MH  - Intravital Microscopy/*methods
MH  - Luminescent Proteins/genetics/*metabolism
MH  - Mice
MH  - Neurons/*chemistry/*physiology
MH  - Neurophysiology/*methods
MH  - Zebrafish
MH  - Red Fluorescent Protein
PMC - PMC4846379
OTO - NOTNLM
OT  - C. elegans
OT  - D. melanogaster
OT  - GECI
OT  - calcium imaging
OT  - fluorescent probes
OT  - mouse
OT  - neuroscience
OT  - protein engineering
OT  - zebrafish
COIS- The other authors declare that no competing interests exist. ERS: DSK, ERS, LLL 
      and KS have applied for a patent on materials and methods related to the red GECI 
      variants (application number US 14/974,483). LLL: DSK, ERS, LLL and KS have 
      applied for a patent on materials and methods related to the red GECI variants 
      (application number US 14/974,483). KS: DSK, ERS, LLL and KS have applied for a 
      patent on materials and methods related to the red GECI variants (application 
      number US 14/974,483). DSK: DSK, ERS, LLL and KS have applied for a patent on 
      materials and methods related to the red GECI variants (application number US 
      14/974,483).
EDAT- 2016/03/25 06:00
MHDA- 2017/11/14 06:00
PMCR- 2016/03/25
CRDT- 2016/03/25 06:00
PHST- 2015/11/01 00:00 [received]
PHST- 2016/03/24 00:00 [accepted]
PHST- 2016/03/25 06:00 [entrez]
PHST- 2016/03/25 06:00 [pubmed]
PHST- 2017/11/14 06:00 [medline]
PHST- 2016/03/25 00:00 [pmc-release]
AID - e12727 [pii]
AID - 12727 [pii]
AID - 10.7554/eLife.12727 [doi]
PST - epublish
SO  - Elife. 2016 Mar 24;5:e12727. doi: 10.7554/eLife.12727.