PMID- 27012032
OWN - NLM
STAT- MEDLINE
DCOM- 20160418
LR  - 20191113
IS  - 1433-6510 (Print)
IS  - 1433-6510 (Linking)
VI  - 62
IP  - 1-2
DP  - 2016
TI  - MicroRNA-153 Regulates NRF2 Expression and is Associated with Breast 
      Carcinogenesis.
PG  - 39-47
AB  - BACKGROUND: Abnormal expression of NRF2 levels in some tumor tissues may enhance 
      drug resistance through various mechanisms. Recent studies have demonstrated a 
      link between dysregulated expression of miRNAs and breast carcinogenesis. 
      METHODS: Quantitative RT-PCR analysis was used to examine the expression levels 
      of miR-153 in breast cancer cell lines. The biological effects of miR-153 were 
      assessed in CRC cell lines using clonogenic cell survival assay, mammosphere 
      formation assay, cell migration assay, 8-OHdG estimation assay, and flow 
      cytometry analysis. Quantitative RT-PCR and western blot analyses were employed 
      to evaluate the expression of miR-153 targets. In this study, we investigated the 
      role of vitamin C in the regulation of miR-153 (miR-153) and its target gene(s) 
      in cell models of mammary carcinogenesis. RESULTS: In human breast cell lines 
      treated with E2, the level of miR-153 was found to be increased. In contrast, 
      vitamin C treatment was able to decrease the expression of miR-153. Bioinformatic 
      prediction indicates nuclear factor erythroid 2-related factor 2 (NRF2) may be a 
      target for miR-153. In E2-treated breast cancer cell lines, NRF2 protein level 
      was found to be decreased. Overexpression of miR-153 significantly reduced NRF2 
      and the downstream genes. Furthermore, miR-153 was found to decrease apoptosis 
      and increase colony formation in breast epithelial cells. CONCLUSIONS: These data 
      indicate that miR-153 acts as an oncogene in breast carcinogenesis by targeting 
      NRF2.
FAU - Wang, Bo
AU  - Wang B
FAU - Teng, Yang
AU  - Teng Y
FAU - Liu, Qilun
AU  - Liu Q
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Clin Lab
JT  - Clinical laboratory
JID - 9705611
RN  - 0 (MIRN153 microRNA, human)
RN  - 0 (MIRN153 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Nfe2l2 protein, rat)
RN  - 4TI98Z838E (Estradiol)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Ascorbic Acid/pharmacology
MH  - Breast Neoplasms/genetics/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Computational Biology
MH  - Estradiol/pharmacology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Mammary Glands, Animal/drug effects/*metabolism/pathology
MH  - MicroRNAs/genetics/*metabolism
MH  - NF-E2-Related Factor 2/genetics/*metabolism
MH  - Oxidative Stress
MH  - RNA Interference
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - Transfection
EDAT- 2016/03/26 06:00
MHDA- 2016/04/19 06:00
CRDT- 2016/03/26 06:00
PHST- 2016/03/26 06:00 [entrez]
PHST- 2016/03/26 06:00 [pubmed]
PHST- 2016/04/19 06:00 [medline]
AID - 10.7754/clin.lab.2015.150518 [doi]
PST - ppublish
SO  - Clin Lab. 2016;62(1-2):39-47. doi: 10.7754/clin.lab.2015.150518.