PMID- 27014654
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160325
LR  - 20220409
IS  - 2277-9175 (Print)
IS  - 2277-9175 (Electronic)
IS  - 2277-9175 (Linking)
VI  - 5
DP  - 2016
TI  - Association between two common polymorphisms (single nucleotide polymorphism 
      -250G/A and -514C/T) of the hepatic lipase gene and coronary artery disease in 
      type 2 diabetic patients.
PG  - 27
LID - 10.4103/2277-9175.176366 [doi]
LID - 27
AB  - BACKGROUND: Variations in the hepatic lipase (HL) gene are the potential 
      candidate for coronary artery disease (CAD) especially in type 2 diabetes 
      mellitus (T2DM) in diverse populations. We assessed the association of -514C/T 
      and -250G/A polymorphisms in HL (LIPC) gene with CAD risk in Iranian population 
      with type 2 diabetes. MATERIALS AND METHODS: We evaluated 322 type 2 diabetic 
      patients, 166 patients with normal angiograms as controls and 156 patients those 
      identified with CAD undergoing their first coronary angiography as CAD cases. 
      Genotyping of -514C/T and -250G/A polymorphisms in the promoter of the LIPC gene 
      were studied by polymerase chain reaction (PCR)-restriction fragment length 
      polymorphism technique. RESULTS: Genotype distributions in CAD cases (73.7%, 
      20.5%, and 5.8% for -250G/A) and (62.2%, 32.7%, and 5.1% for -514C/T) were 
      significantly different from those in controls (60.8%, 37.4%, and 1.8% for 
      -250G/A) and (51.2%, 48.2%, and 0.6% for -514C/T). CAD cases had lower A-allele 
      frequency than controls (0.131 vs. 0.196, P = 0.028). The odds ratio for the 
      presence of -250 (GG + GA) genotype and A allele in CAD cases were 2.206 (95% 
      confidence interval [CI] =1.33-3.65, P = 0.002) and 1.609 (95% CI = 1.051 -2.463, 
      P = 0.029) respectively. Haplotype analysis demonstrated a significant 
      association between especially LIPC double mutant (-250 A/-514 T) haplotype and 
      presence of CAD. CONCLUSION: Our findings indicated that -250 G/A polymorphism 
      rather than -514 C/T polymorphism of LIPC gene is more associated with the 
      increased risk of CAD particularly in women with T2DM.
FAU - Mohammadzadeh, Ghorban
AU  - Mohammadzadeh G
AD  - Hyperlipidemia Research Center, Department of Clinical Biochemistry, Ahvaz 
      Jundishapur University of Medical Sciences, Ahvaz, Iran.
FAU - Ghaffari, Mohammad-Ali
AU  - Ghaffari MA
AD  - Cellular and Molecular Research Center, Department of Clinical Biochemistry, 
      Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
FAU - Bazyar, Mohammad
AU  - Bazyar M
AD  - Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur 
      University of Medical Sciences, Ahvaz, Iran.
FAU - Kheirollah, Alireza
AU  - Kheirollah A
AD  - Cellular and Molecular Research Center, Department of Clinical Biochemistry, 
      Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
LA  - eng
PT  - Journal Article
DEP - 20160215
PL  - India
TA  - Adv Biomed Res
JT  - Advanced biomedical research
JID - 101586897
PMC - PMC4785784
OTO - NOTNLM
OT  - LIPC gene
OT  - Type 2 diabetes
OT  - coronary artery disease
OT  - polymorphisms
COIS- Conflict of Interest: None declared.
EDAT- 2016/03/26 06:00
MHDA- 2016/03/26 06:01
PMCR- 2016/02/15
CRDT- 2016/03/26 06:00
PHST- 2014/10/23 00:00 [received]
PHST- 2015/02/24 00:00 [accepted]
PHST- 2016/03/26 06:00 [entrez]
PHST- 2016/03/26 06:00 [pubmed]
PHST- 2016/03/26 06:01 [medline]
PHST- 2016/02/15 00:00 [pmc-release]
AID - ABR-5-27 [pii]
AID - 10.4103/2277-9175.176366 [doi]
PST - epublish
SO  - Adv Biomed Res. 2016 Feb 15;5:27. doi: 10.4103/2277-9175.176366. eCollection 
      2016.