PMID- 27016768
OWN - NLM
STAT- MEDLINE
DCOM- 20161021
LR  - 20161230
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 186
DP  - 2016 Apr 15
TI  - Potential bacterial core species associated with digital dermatitis in cattle 
      herds identified by molecular profiling of interdigital skin samples.
PG  - 139-49
LID - S0378-1135(16)30056-6 [pii]
LID - 10.1016/j.vetmic.2016.03.003 [doi]
AB  - Although treponemes are consistently identified in tissue from bovine digital 
      dermatitis (DD) lesions, the definitive etiology of this debilitating 
      polymicrobial disease is still unresolved. To study the microbiomes of 27 
      DD-infected and 10 healthy interdigital skin samples, we used a combination of 
      different molecular methods. Deep sequencing of the 16S rRNA gene variable 
      regions V1-V2 showed that Treponema, Mycoplasma, Fusobacterium and Porphyromonas 
      were the genera best differentiating the DD samples from the controls. Additional 
      deep sequencing analysis of the most abundant genus, Treponema, targeting another 
      variable region of the 16S rRNA gene, V3-V4, identified 15 different phylotypes, 
      among which Treponema phagedenis-like and Treponema refringens-like species were 
      the most abundant. Although the presence of Treponema spp., Fusobacterium 
      necrophorum and Porphyromonas levii was confirmed by fluorescence in situ 
      hybridization (FISH), the results for Mycoplasma spp. were inconclusive. 
      Extensive treponemal epidermal infiltration, constituting more than 90% of the 
      total bacterial population, was observed in 24 of the 27 DD samples. F. 
      necrophorum and P. levii were superficially located in the epidermal lesions and 
      were present in only a subset of samples. RT-qPCR analysis showed that treponemes 
      were also actively expressing a panel of virulence factors at the site of 
      infection. Our results further support the hypothesis that species belonging to 
      the genus Treponema are major pathogens of DD and also provide sufficient clues 
      to motivate additional research into the role of M. fermentans, F. necrophorum 
      and P. levii in the etiology of DD.
CI  - Copyright (c) 2016 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Nielsen, Martin W
AU  - Nielsen MW
AD  - National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, 
      1870 Frederiksberg C, Denmark.
FAU - Strube, Mikael L
AU  - Strube ML
AD  - National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, 
      1870 Frederiksberg C, Denmark.
FAU - Isbrand, Anastasia
AU  - Isbrand A
AD  - National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, 
      1870 Frederiksberg C, Denmark.
FAU - Al-Medrasi, Worood D H M
AU  - Al-Medrasi WD
AD  - National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, 
      1870 Frederiksberg C, Denmark.
FAU - Boye, Mette
AU  - Boye M
AD  - National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, 
      1870 Frederiksberg C, Denmark.
FAU - Jensen, Tim K
AU  - Jensen TK
AD  - National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, 
      1870 Frederiksberg C, Denmark.
FAU - Klitgaard, Kirstine
AU  - Klitgaard K
AD  - National Veterinary Institute, Technical University of Denmark, Bulowsvej 27, 
      1870 Frederiksberg C, Denmark. Electronic address: kksc@vet.dtu.dk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160306
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - Biodiversity
MH  - Cattle
MH  - Cattle Diseases/*microbiology/pathology
MH  - Digital Dermatitis/*microbiology
MH  - Gram-Negative Bacteria/genetics/isolation & purification/*physiology
MH  - Gram-Negative Bacterial Infections/microbiology/pathology/*veterinary
MH  - Microbiota/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Skin/*microbiology
MH  - Virulence Factors/genetics
OTO - NOTNLM
OT  - 16S rRNA
OT  - Bovine digital dermatitis
OT  - Microbiome
OT  - Next generation sequencing
OT  - RT-qPCR
OT  - Treponema
EDAT- 2016/03/29 06:00
MHDA- 2016/10/22 06:00
CRDT- 2016/03/28 06:00
PHST- 2016/01/05 00:00 [received]
PHST- 2016/03/02 00:00 [revised]
PHST- 2016/03/04 00:00 [accepted]
PHST- 2016/03/28 06:00 [entrez]
PHST- 2016/03/29 06:00 [pubmed]
PHST- 2016/10/22 06:00 [medline]
AID - S0378-1135(16)30056-6 [pii]
AID - 10.1016/j.vetmic.2016.03.003 [doi]
PST - ppublish
SO  - Vet Microbiol. 2016 Apr 15;186:139-49. doi: 10.1016/j.vetmic.2016.03.003. Epub 
      2016 Mar 6.