PMID- 27017919
OWN - NLM
STAT- MEDLINE
DCOM- 20170705
LR  - 20181113
IS  - 1878-5867 (Electronic)
IS  - 0039-128X (Print)
IS  - 0039-128X (Linking)
VI  - 111
DP  - 2016 Jul
TI  - Analyses of rapid estrogen actions on rat ventromedial hypothalamic neurons.
PG  - 100-112
LID - S0039-128X(16)00077-5 [pii]
LID - 10.1016/j.steroids.2016.03.010 [doi]
AB  - Rapid estrogen actions are widely diverse across many cell types. We conducted a 
      series of electrophysiological studies on single rat hypothalamic neurons and 
      found that estradiol (E2) could rapidly and independently potentiate neuronal 
      excitation/depolarizations induced by histamine (HA) and N-Methyl-d-Aspartate 
      (NMDA). Now, the present whole-cell patch study was designed to determine whether 
      E2 potentiates HA and NMDA depolarizations - mediated by distinctly different 
      types of receptors - by the same or by different mechanisms. For this, the 
      actions of HA, NMDA, as well as E2, were investigated first using various ion 
      channel blockers and then by analyzing and comparing their channel activating 
      characteristics. Results indicate that: first, both HA and NMDA depolarize 
      neurons by inhibiting K(+) currents. Second, E2 potentiates both HA and NMDA 
      depolarizations by enhancing the inhibition of K(+) currents, an inhibition 
      caused by the two transmitters. Third, E2 employs the very same mechanism, the 
      enhancement of K(+) current inhibition, thus to rapidly potentiate HA and NMDA 
      depolarizations. These data are of behavioral importance, since the rapid E2 
      potentiation of depolarization synergizes with nuclear genomic actions of E2 to 
      facilitate lordosis behavior, the primary female-typical reproductive behavior.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Kow, Lee-Ming
AU  - Kow LM
AD  - Laboratory of Neurobiology & Behavior, The Rockefeller University, New York, NY 
      10065, USA. Electronic address: kowl@rockefeller.edu.
FAU - Pataky, Stefan
AU  - Pataky S
AD  - Laboratory of Neurobiology & Behavior, The Rockefeller University, New York, NY 
      10065, USA. Electronic address: patakys@gmail.com.
FAU - Dupre, Christophe
AU  - Dupre C
AD  - Laboratory of Neurobiology & Behavior, The Rockefeller University, New York, NY 
      10065, USA. Electronic address: cd2597@columbia.edu.
FAU - Phan, Anna
AU  - Phan A
AD  - Laboratory of Neurobiology & Behavior, The Rockefeller University, New York, NY 
      10065, USA. Electronic address: aphan@scripps.edu.
FAU - Martin-Alguacil, Nieves
AU  - Martin-Alguacil N
AD  - Laboratory of Neurobiology & Behavior, The Rockefeller University, New York, NY 
      10065, USA. Electronic address: nmartina@ucm.es.
FAU - Pfaff, Donald W
AU  - Pfaff DW
AD  - Laboratory of Neurobiology & Behavior, The Rockefeller University, New York, NY 
      10065, USA.
LA  - eng
GR  - R01 HD005751/HD/NICHD NIH HHS/United States
GR  - R37 HD005751/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20160324
PL  - United States
TA  - Steroids
JT  - Steroids
JID - 0404536
RN  - 0 (Estrogens)
RN  - 4TI98Z838E (Estradiol)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Estradiol/pharmacology
MH  - Estrogens/*pharmacology
MH  - Female
MH  - Histamine/pharmacology
MH  - Hypothalamus/*cytology
MH  - Membrane Potentials/drug effects
MH  - N-Methylaspartate/pharmacology
MH  - Neurons/*drug effects/*metabolism
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC4965276
MID - NIHMS774035
OTO - NOTNLM
OT  - Estradiol
OT  - Histamine
OT  - Ion channel
OT  - N-Methyl-d-aspartate (NMDA)
OT  - Neuronal activity
OT  - Patch clamp
OT  - Synergism
EDAT- 2016/03/29 06:00
MHDA- 2017/07/06 06:00
PMCR- 2017/07/01
CRDT- 2016/03/29 06:00
PHST- 2016/01/22 00:00 [received]
PHST- 2016/02/26 00:00 [revised]
PHST- 2016/03/11 00:00 [accepted]
PHST- 2016/03/29 06:00 [entrez]
PHST- 2016/03/29 06:00 [pubmed]
PHST- 2017/07/06 06:00 [medline]
PHST- 2017/07/01 00:00 [pmc-release]
AID - S0039-128X(16)00077-5 [pii]
AID - 10.1016/j.steroids.2016.03.010 [doi]
PST - ppublish
SO  - Steroids. 2016 Jul;111:100-112. doi: 10.1016/j.steroids.2016.03.010. Epub 2016 
      Mar 24.